Preprint Article Version 1 This version is not peer-reviewed

The Use of Translocator protein 18 kDa (Tspo) as a Biomarker in Various Human Cancers

Version 1 : Received: 30 May 2018 / Approved: 31 May 2018 / Online: 31 May 2018 (10:59:31 CEST)

A peer-reviewed article of this Preprint also exists.

Bhoola, N.H.; Mbita, Z.; Hull, R.; Dlamini, Z. Translocator Protein (TSPO) as a Potential Biomarker in Human Cancers. Int. J. Mol. Sci. 2018, 19, 2176. Bhoola, N.H.; Mbita, Z.; Hull, R.; Dlamini, Z. Translocator Protein (TSPO) as a Potential Biomarker in Human Cancers. Int. J. Mol. Sci. 2018, 19, 2176.

Journal reference: Int. J. Mol. Sci. 2018, 19, 2176
DOI: 10.3390/ijms19082176

Abstract

Tspo is a receptor involved in the regulation of cellular proliferation, apoptosis and mitochondrial functions. Previous studies showed the expression of TSPO protein correlated positively with tumour malignancy and negatively with patient survival. The aim of this study was to determine the transcription of Tspo mRNA in various types of normal and cancer tissues. In situ hybridization was performed to localise the Tspo mRNA in various human normal and cancer tissues. The relative level of Tspo mRNA was quantified using fluorescent intensity and visual estimation of colorimetric staining. RT-PCR was used to confirm these mRNA levels in normal lung, lung cancer, liver cancer and cervical cancer cell lines. There was a significant increase in the level of transcription in liver, prostate, kidney and brain cancers while a significant decrease was observed in cancers of the colon and lung. Quantitative RT-PCR confirmed that the mRNA levels of Tspo are higher in a normal lung cell line than in a lung cancer cell line. An increase in the expression levels of TSPO makes it a good diagnostic biomarker and TSPO could serve as a target for anticancer drug development.

Subject Areas

Tspo; prostate cancer; stomach cancer; colon cancer; liver cancer; lung cancer; kidney cancer; breast cancer; brain cancer

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