Version 1
: Received: 13 May 2018 / Approved: 14 May 2018 / Online: 14 May 2018 (12:12:07 CEST)
How to cite:
Roni, M.Z.K.; Islam, M.S.; Shimasaki, K. In Vitro Seedlings of Eustoma grandiflorum in Response to LED Light in an Acclimation Environment. Preprints2018, 2018050194. https://doi.org/10.20944/preprints201805.0194.v1
Roni, M.Z.K.; Islam, M.S.; Shimasaki, K. In Vitro Seedlings of Eustoma grandiflorum in Response to LED Light in an Acclimation Environment. Preprints 2018, 2018050194. https://doi.org/10.20944/preprints201805.0194.v1
Roni, M.Z.K.; Islam, M.S.; Shimasaki, K. In Vitro Seedlings of Eustoma grandiflorum in Response to LED Light in an Acclimation Environment. Preprints2018, 2018050194. https://doi.org/10.20944/preprints201805.0194.v1
APA Style
Roni, M.Z.K., Islam, M.S., & Shimasaki, K. (2018). <em>In Vitro S</em>eedlings of <em>Eustoma grandiflorum</em> in Response to LED Light in an Acclimation Environment. Preprints. https://doi.org/10.20944/preprints201805.0194.v1
Chicago/Turabian Style
Roni, M.Z.K., Md Saiful Islam and Kazuhiko Shimasaki. 2018 "<em>In Vitro S</em>eedlings of <em>Eustoma grandiflorum</em> in Response to LED Light in an Acclimation Environment" Preprints. https://doi.org/10.20944/preprints201805.0194.v1
Abstract
Transferring in vitro-cultured Eustoma seedlings to an ex vitro condition (acclimation) is a big challenge that may expose the seedlings to biotic and abiotic stresses, and affect the internal and external structure of the plants. In addition, in vitro-cultured seedlings of Eustoma are difficult to handle and phenotype and physiological traits such as survival and rosette rate may have altered in the acclimation stage. Therefore, the present study aims to examine the effects of blue, red, and white LED light on the growth and development ex vitro of in vitro-cultured seedlings of Eustoma. The results showed that blue LEDs resulted in greater plant height, internode length, and leaf number, increased upper and lower fresh biomass, and higher chlorophyll content compared with treatment by the other LED lights. Higher stomatal density on the abaxial leaf surface was also observed in the blue LED-treated plants, which also showed a higher survival rate and lower rosette rate. In contrast, the white LED-treated plants had the highest leaf width and internode diameter. Acclimation of the Eustoma plants ex vitro suggests that a combination of blue and white LEDs may be advantageous for better growth and development for large-scale production in a controlled environment.
Keywords
ex vitro; internode; light-emitting diodes; stomata; rosette
Subject
Biology and Life Sciences, Horticulture
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
