Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Polyphenols from Tamarix nilotica: LC–ESI-MSn Profiling and In-Vivo Antifibrotic Activity

Version 1 : Received: 9 May 2018 / Approved: 11 May 2018 / Online: 11 May 2018 (05:18:58 CEST)

A peer-reviewed article of this Preprint also exists.

Sekkien, A.; Swilam, N.; Ebada, S.S.; Esmat, A.; El-Khatib, A.H.; Linscheid, M.W.; Singab, A.N. Polyphenols from Tamarix nilotica: LC–ESI-MSn Profiling and In Vivo Antifibrotic Activity. Molecules 2018, 23, 1411. Sekkien, A.; Swilam, N.; Ebada, S.S.; Esmat, A.; El-Khatib, A.H.; Linscheid, M.W.; Singab, A.N. Polyphenols from Tamarix nilotica: LC–ESI-MSn Profiling and In Vivo Antifibrotic Activity. Molecules 2018, 23, 1411.

Abstract

Tamarix nilotica (Ehrenb.) Bunge (Tamaricaceae), an indigenous plant to the Middle East region, is well-known as a medicinal plant for treating many human ailments. The current study aimed at exploring the polyphenols profile of T.nilotica alcohol soluble fraction of aqueous extract, assessing its in-vivo antifibrotic activity and the possible underlying mechanism, to unravel the impact of quantitative difference of sulphated polyphenols content on the antifibrotic activity of T.nilotca grown in two different habitats. Polyphenols profiling of T. nilotica extracts was performed using HPLC-HRESI-QTOF-MS-MS. The major polyphenol components included sulphated flavonoids, phenolic acids and free aglycones. The antifibrotic activity was evaluated through carbon tetrachloride-induced liver fibrosis in rats. Biochemical evaluations revealed that both fractions ameliorated the increased levels of hepatic aminotransferases, lipid peroxidation, hydroxyproline, α-smooth muscle actin (α-SMA), tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2) and nuclear factor kappa B (NF-κB). Moreover, both fractions reduced catalase activity (CAT) and enhanced hepatic glutathione (GSH) content. Histopathological imaging undoubtedly confirmed such results. In conclusion, T. nilotica polyphenols rich fraction exhibited potential antifibrotic activity in rats. Significant alterations in GSH levels were recorded based on sulphated polyphenol metabolites content.

Keywords

Tamarix nilotica; Tamaricaceae; polyphenols; HPLC/HRESI/MS/MS; antifibrotic activity

Subject

Medicine and Pharmacology, Pharmacology and Toxicology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.