Version 1
: Received: 29 April 2018 / Approved: 30 April 2018 / Online: 30 April 2018 (18:44:25 CEST)
How to cite:
Pinelli, S.; Poli, D.; Alinovi, R.; Banda, I.; Pelosi, G.; Petyx, M.; Iavicoli, S.; Mutti, A.; Mozzoni, P.; Goldoni, M. Effects of TiO2 and Co3O4 Nanoparticles on Cell Cycle of a Pulmonary Cell Line (A549). Preprints2018, 2018040384. https://doi.org/10.20944/preprints201804.0384.v1
Pinelli, S.; Poli, D.; Alinovi, R.; Banda, I.; Pelosi, G.; Petyx, M.; Iavicoli, S.; Mutti, A.; Mozzoni, P.; Goldoni, M. Effects of TiO2 and Co3O4 Nanoparticles on Cell Cycle of a Pulmonary Cell Line (A549). Preprints 2018, 2018040384. https://doi.org/10.20944/preprints201804.0384.v1
Pinelli, S.; Poli, D.; Alinovi, R.; Banda, I.; Pelosi, G.; Petyx, M.; Iavicoli, S.; Mutti, A.; Mozzoni, P.; Goldoni, M. Effects of TiO2 and Co3O4 Nanoparticles on Cell Cycle of a Pulmonary Cell Line (A549). Preprints2018, 2018040384. https://doi.org/10.20944/preprints201804.0384.v1
APA Style
Pinelli, S., Poli, D., Alinovi, R., Banda, I., Pelosi, G., Petyx, M., Iavicoli, S., Mutti, A., Mozzoni, P., & Goldoni, M. (2018). Effects of TiO<sub>2</sub> and Co<sub>3</sub>O<sub>4 </sub>Nanoparticles on Cell Cycle of a Pulmonary Cell Line (A549). Preprints. https://doi.org/10.20944/preprints201804.0384.v1
Chicago/Turabian Style
Pinelli, S., Paola Mozzoni and Matteo Goldoni. 2018 "Effects of TiO<sub>2</sub> and Co<sub>3</sub>O<sub>4 </sub>Nanoparticles on Cell Cycle of a Pulmonary Cell Line (A549)" Preprints. https://doi.org/10.20944/preprints201804.0384.v1
Abstract
The wide range of applications of nanoparticles (NPs) has increased the probability of environmental and occupational exposure. In a previous comparative study on cell line A549, we observed that oxidative stress caused by Co3O4 NPs affects the energetic homeostasis and the detoxification capacity, preventing autophagy induced by TiO2 NPs. In this study, we have investigated the effects of NPs on the cell cycle.Cytofluorimetric analysis showed a slow-down of the cell cycle progression for both NPs, with increases in the percentage of resting cells in the G0/G1. These observations were confirmed by a reduced expression of all cyclins, especially of CCNE1 and CDK2, involved in the late stages of the G1 phase, coupled with a significant increase in the expression of p21 only for Co3O4 NP exposure. On the contrary, the effects of TiO2 NPs were modest. Cell cycle related miRNA-34a, miRNA-126 and miRNA-1290 resulted increased at different early time-points (4-8h) but were down-expressed at 24h-48h only after TiO2 NP exposure. Our results show that NPs have an antiproliferative effect and deregulate cell cycle on A549 cells. These effects should not be underestimated because regulation of cycle progression is crucial for cell survival and repair of genetic damage.
Keywords
TiO2; Co3O4; Nanoparticles; Cell Cycle; microRNA
Subject
Medicine and Pharmacology, Pharmacology and Toxicology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
