Version 1
: Received: 26 April 2018 / Approved: 27 April 2018 / Online: 27 April 2018 (08:36:00 CEST)
How to cite:
Esteban, T.; Atucha, N. M.; Moraleda, J. M.; García-Estañ, J.; Insausti, C. L.; Moraleda-Deleito, J. Chronification of Tympanic Perforation with Mitomycin C in a Rat Model. Preprints2018, 2018040356. https://doi.org/10.20944/preprints201804.0356.v1
Esteban, T.; Atucha, N. M.; Moraleda, J. M.; García-Estañ, J.; Insausti, C. L.; Moraleda-Deleito, J. Chronification of Tympanic Perforation with Mitomycin C in a Rat Model. Preprints 2018, 2018040356. https://doi.org/10.20944/preprints201804.0356.v1
Esteban, T.; Atucha, N. M.; Moraleda, J. M.; García-Estañ, J.; Insausti, C. L.; Moraleda-Deleito, J. Chronification of Tympanic Perforation with Mitomycin C in a Rat Model. Preprints2018, 2018040356. https://doi.org/10.20944/preprints201804.0356.v1
APA Style
Esteban, T., Atucha, N. M., Moraleda, J. M., García-Estañ, J., Insausti, C. L., & Moraleda-Deleito, J. (2018). Chronification of Tympanic Perforation with Mitomycin C in a Rat Model. Preprints. https://doi.org/10.20944/preprints201804.0356.v1
Chicago/Turabian Style
Esteban, T., Carmen L. Insausti and Javier Moraleda-Deleito. 2018 "Chronification of Tympanic Perforation with Mitomycin C in a Rat Model" Preprints. https://doi.org/10.20944/preprints201804.0356.v1
Abstract
Background. A rat model of chronic tympanic membrane perforation was developed to be used in the search of new materials for the sealing of these perforations. Methods. A longitudinal study was carried out in rats subjected to incisional myringotomy followed by the application of mitomycin C alone or with dexamethasone. Rats were checked at days 3, 7, 10, 14 and weekly thereafter until perforation closure, for up to 6 months. Results. The addition of dexamethasone is a key component in order to obtain a chronic opening. Myringotomies treated with saline had a mean healing time of 8.5 days. At 8 weeks, 70.5% of these remained perforated and at 6 months this number fell to 21.4%. Conclusion. This technique is able to maintain more than 70% of tympanic membrane perforations patent for at least 8 weeks. This rat model is adequate for its use in preclinical or translational research.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Commenter:
Commenter's Conflict of Interests: I am one of the authors