Preprint Article Version 1 This version is not peer-reviewed

Antifungal Activity of G. africana Extract against Candida Strains

Version 1 : Received: 12 April 2018 / Approved: 13 April 2018 / Online: 13 April 2018 (03:49:28 CEST)

How to cite: Ng’uni, T.L.; dos Santos Abrantes, P.M.; McArthur, C.; Klaasen, J.A.; Fielding, B.C. Antifungal Activity of G. africana Extract against Candida Strains. Preprints 2018, 2018040171 (doi: 10.20944/preprints201804.0171.v1). Ng’uni, T.L.; dos Santos Abrantes, P.M.; McArthur, C.; Klaasen, J.A.; Fielding, B.C. Antifungal Activity of G. africana Extract against Candida Strains. Preprints 2018, 2018040171 (doi: 10.20944/preprints201804.0171.v1).

Abstract

Candida infections have increased in recent years and are causing serious public health concern. In addition, Candida species are becoming resistant to numerous antifungal drugs. It is for this reason that alternative treatment options are being explored, using medicinal plants, to curb this trend of drug resistance. The aim of this study was to evaluate the anti-Candida activity of an ethanolic extract of Galenia africana (G. africana) alone and particularly in combination with fluconazole (FLC) against Candida albicans and Candida glabrata. The anti-Candida activity was evaluated using various techniques. The minimum inhibitory concentration (MIC) of the extract was 6.25 mg/ml for both Candida strains. After combining with FLC, G. africana exerted a strong synergistic effect against C. albicans and an indifferent effect against C. glabrata when interpreted by the fractional inhibitory concentration index (FICI) (0.36 and 1.002 for C. albicans and C. glabrata, respectively). Microscopic analysis revealed cell damage and decrease in cell size after G. africana treatment. Collectively, these results suggested that G. africana possessed antifungal activities against the Candida strains and a synergistic effect with FLC. Cell damage observed possibly contributed to this synergistic effect. This provides new information for the development of new antifungal agents.

Subject Areas

drug resistance; Candida albicans; Candida glabrata; Galenia africana; fluconazole; broth microdilution; checkerboard; sensititre

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