Preprint Short Note Version 1 This version is not peer-reviewed

Effects of Lyophilization and Storage Temperature on Wuchereria bancrofti Antigen Sensitivity and Stability

Version 1 : Received: 12 April 2018 / Approved: 12 April 2018 / Online: 12 April 2018 (16:18:09 CEST)

How to cite: Agbozo, E.Y.; Dumashie, E.; Boakye, D.A.; de Souza, D.K. Effects of Lyophilization and Storage Temperature on Wuchereria bancrofti Antigen Sensitivity and Stability. Preprints 2018, 2018040170 (doi: 10.20944/preprints201804.0170.v1). Agbozo, E.Y.; Dumashie, E.; Boakye, D.A.; de Souza, D.K. Effects of Lyophilization and Storage Temperature on Wuchereria bancrofti Antigen Sensitivity and Stability. Preprints 2018, 2018040170 (doi: 10.20944/preprints201804.0170.v1).

Abstract

Antigen-based rapid diagnostic tests for Lymphatic filariasis do not come with quality control (QC) materials, and research and disease control programmes rely on stored positive plasma samples. This study was undertaken to evaluate the use of freeze-dried Wuchereria bancrofti antigen positive plasma samples to serve as QC materials for LF RDTs. 10 well characterized W. bancrofti positive samples were lyophilized and stored at 4°C, 28°C and 40°C. The samples were evaluated using the Filaria Test Strips before lyophilization and after one and three (3) months of storage. The sensitivity and stability of the lyophilized samples were evaluated. The results revealed a loss of sensitivity and stability with increasing temperature and duration of storage. The results are further discussed in terms of the use of Dried Blood Spot (DBS) in diagnostics studies on LF requiring quantitative assessments, and the need for thoughtful DBS preparation and storage.

Subject Areas

Wuchereria bancrofti antigen, lyophilization, dried blood spots, freeze-drying

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