PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Anti-Proliferation, Pro-Apoptosis and Anti-Migration Effects of Ginkgolic Acid C13:0 Isolated from Ginkgo Biloba Exocarp in MCF-7 and 4T-1 Breast Cancer Cells
Version 1
: Received: 27 January 2018 / Approved: 29 January 2018 / Online: 29 January 2018 (09:57:10 CET)
How to cite:
Zhou, D.; Jiang, C.; Fu, C.; Chang, P.; Wu, J.; Zheng, K.; Zhao, X.; Ma, S. Anti-Proliferation, Pro-Apoptosis and Anti-Migration Effects of Ginkgolic Acid C13:0 Isolated from Ginkgo Biloba Exocarp in MCF-7 and 4T-1 Breast Cancer Cells. Preprints2018, 2018010271. https://doi.org/10.20944/preprints201801.0271.v1
Zhou, D.; Jiang, C.; Fu, C.; Chang, P.; Wu, J.; Zheng, K.; Zhao, X.; Ma, S. Anti-Proliferation, Pro-Apoptosis and Anti-Migration Effects of Ginkgolic Acid C13:0 Isolated from Ginkgo Biloba Exocarp in MCF-7 and 4T-1 Breast Cancer Cells. Preprints 2018, 2018010271. https://doi.org/10.20944/preprints201801.0271.v1
Zhou, D.; Jiang, C.; Fu, C.; Chang, P.; Wu, J.; Zheng, K.; Zhao, X.; Ma, S. Anti-Proliferation, Pro-Apoptosis and Anti-Migration Effects of Ginkgolic Acid C13:0 Isolated from Ginkgo Biloba Exocarp in MCF-7 and 4T-1 Breast Cancer Cells. Preprints2018, 2018010271. https://doi.org/10.20944/preprints201801.0271.v1
APA Style
Zhou, D., Jiang, C., Fu, C., Chang, P., Wu, J., Zheng, K., Zhao, X., & Ma, S. (2018). Anti-Proliferation, Pro-Apoptosis and Anti-Migration Effects of Ginkgolic Acid C13:0 Isolated from Ginkgo Biloba Exocarp in MCF-7 and 4T-1 Breast Cancer Cells. Preprints. https://doi.org/10.20944/preprints201801.0271.v1
Chicago/Turabian Style
Zhou, D., Xiao-Hui Zhao and Shi-Liang Ma. 2018 "Anti-Proliferation, Pro-Apoptosis and Anti-Migration Effects of Ginkgolic Acid C13:0 Isolated from Ginkgo Biloba Exocarp in MCF-7 and 4T-1 Breast Cancer Cells" Preprints. https://doi.org/10.20944/preprints201801.0271.v1
Abstract
Ginkgolic acids (GA) have been reported to exhibit anticancer properties, however, the mechanisms remain unclear. This study aims to investigate the mechanisms of GA C13:0 that was isolated from Ginkgo biloba exocarp (GBE) for anti-proliferation, pro-apoptosis and anti-migration effects in human MCF-7 and mouse 4T-1 breast cancer cells. The cytotoxic effect, apoptosis induction and migration inhibition were measured using MTT, TUNEL and Wound healing assays. The expression of mRNA and protein were determined using qPCR and Western blot. Our results showed that no cytotoxicity was found at concentrations of C13:0 below 100µM. The effects of GA C13:0 was further demonstrated by up-regulation of the Bax/Bcl-2 apoptosis pathway and the expression of Apaf-1 protein in the mitochondria. In addition, GA C13:0 also suppressed cell migration and epithelial to mesenchymal transition (EMT) with the increase of E-cadherin expression accompanied by the decrease of Snail, MMP-2, MMP-9 and Vimentin expression. Moreover, GA C13:0 induced cytochrome P450 (CYP) 1B1 expression in aryl hydrocarbon receptor (AhR) pathway. Notably, the up-regulation of CYP1B1 also might play a pivotal regulatory role in mitochondrial and EMT pathways in MCF-7 and 4T-1 cells. Our results may have implications for the development of anticancer agents containing GA as functional additives.
Keywords
breast cancer; ginkgolic acid C13:0; mechanism; CYP1B1
Subject
Medicine and Pharmacology, Pharmacology and Toxicology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
