Version 1
: Received: 7 January 2018 / Approved: 9 January 2018 / Online: 9 January 2018 (03:14:28 CET)
How to cite:
Sargolzaei Aval, F.; Saberi, E.; Arab, M. R.; Sargolzaei, N.; Zare, E.; Shahraki, H.; Sargolzaeiaval, F.; Arab, M. Histological Assessment of Bone Regeneration by Octacalcium Phosphate and Bone Matrix Gelatin Composites in a Rat Mandibular Defect Model. Preprints2018, 2018010062. https://doi.org/10.20944/preprints201801.0062.v1
Sargolzaei Aval, F.; Saberi, E.; Arab, M. R.; Sargolzaei, N.; Zare, E.; Shahraki, H.; Sargolzaeiaval, F.; Arab, M. Histological Assessment of Bone Regeneration by Octacalcium Phosphate and Bone Matrix Gelatin Composites in a Rat Mandibular Defect Model. Preprints 2018, 2018010062. https://doi.org/10.20944/preprints201801.0062.v1
Sargolzaei Aval, F.; Saberi, E.; Arab, M. R.; Sargolzaei, N.; Zare, E.; Shahraki, H.; Sargolzaeiaval, F.; Arab, M. Histological Assessment of Bone Regeneration by Octacalcium Phosphate and Bone Matrix Gelatin Composites in a Rat Mandibular Defect Model. Preprints2018, 2018010062. https://doi.org/10.20944/preprints201801.0062.v1
APA Style
Sargolzaei Aval, F., Saberi, E., Arab, M. R., Sargolzaei, N., Zare, E., Shahraki, H., Sargolzaeiaval, F., & Arab, M. (2018). Histological Assessment of Bone Regeneration by Octacalcium Phosphate and Bone Matrix Gelatin Composites in a Rat Mandibular Defect Model. Preprints. https://doi.org/10.20944/preprints201801.0062.v1
Chicago/Turabian Style
Sargolzaei Aval, F., Forough Sargolzaeiaval and Maryam Arab. 2018 "Histological Assessment of Bone Regeneration by Octacalcium Phosphate and Bone Matrix Gelatin Composites in a Rat Mandibular Defect Model" Preprints. https://doi.org/10.20944/preprints201801.0062.v1
Abstract
Objective: Regeneration of bone defects remains a challenge for maxillofacial and reparative surgeons. The purpose of this histological study was to assess the osteogenic potential of octacalcium phosphate (OCP) and bone matrix gelatin (BMG) alone and in combination in artificially created mandibular bone defects in rats. The quality of the newly formed bone was also evaluated. Methods: Thirty-six male Sprague Dawley rats (6-8 weeks old with 120-150 g weight) were randomly divided into four groups. Defects (3 mm in diameter and 2 mm in depth) were created in the mandible of rats and filled with 6 mg of OCP, BMG or a combination of both (1/4 ratio), respectively. Defects were left unfilled in the control group. To assess osteoinduction and bone regeneration and determine the quality of the newly formed bone, tissue specimens were harvested at seven, 14, and 21 days post-implantation. The specimens were processed, stained with hematoxylin and eosin (H&E) and histologically analyzed under light microscopy. Results: In the experimental groups, new bone formation was initiated at the margins of defects from seventh day after implantation. At the end of the study period, the amount of the newly formed bone increased and the bone was relatively mature. Osteoinduction and new bone formation were greater in OCP/BMG group. In the control group, slight amount of new bone had been formed at the defect margins (next to host bone) on day 21. Conclusion: Combination of OCP/BMG may serve as an optimal biomaterial for treatment of mandibular bone defects.
Keywords
octacalcium phosphate; bone matrix gelatin; osteogenesis
Subject
Biology and Life Sciences, Anatomy and Physiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
