A Fixed Combination of Probiotics and Herbal Extracts Attenuates Intestinal Barrier Dysfunction from Inflammatory Stress

Veronica Cocetta; Daniela Catanzaro; Vittoria Borgonetti; Eugenio Ragazzi; Maria Cecilia Giron; Paolo Governa; Ilaria Carnevali; Marco Biagi; Monica Montopoli

doi:10.20944/preprints201712.0199.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 29 December 2017 / Approved: 29 December 2017 / Online: 29 December 2017 (08:36:36 CET)

Maintenance of intestinal barrier integrity is crucial for preventing inflammatory bowel diseases (IBDs) onset and exacerbations. In this work we study the effect of a fixed combination of Lactobacillus reuteri and Lactobacillus acidophilus and herbal extracts in an in vitro inflammation experimental model. Caco-2 cell monolayer was exposed to INF-γ+TNF-α or to LPS; Trans Epithelial Electrical Resistance (TEER) and paracellular permeability were investigated. ZO-1 and occludin tight junctions (TJs) were also investigated by mean of immunofluorescence. The pre-treatment with the fixed combination of probiotics and herbal extracts prevented the inflammation-induced TEER decrease, paracellular permeability increase and TJs translocation. In summary the fixed combination of probiotics and herbal extracts investigated in this research was found to be an interesting candidate for targeting the re-establishment of intestinal barrier function in IBDs conditions.

IBD; intestinal barrier; Lactobacillus reuteri; Lactobacillus acidophilus; Trans Epithelial Electrical Resistance (TEER)

MEDICINE & PHARMACOLOGY, Nutrition

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A Fixed Combination of Probiotics and Herbal Extracts Attenuates Intestinal Barrier Dysfunction from Inflammatory Stress

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