A Fixed Combination of Probiotics and Herbal Extracts Attenuates Intestinal Barrier Dysfunction from Inflammatory Stress

Veronica Cocetta; Daniela Catanzaro; Vittoria Borgonetti; Eugenio Ragazzi; Maria Cecilia Giron; Paolo Governa; Ilaria Carnevali; Marco Biagi; Monica Montopoli

doi:10.20944/preprints201712.0199.v1

Preprint Article Version 1 This version is not peer-reviewed

A Fixed Combination of Probiotics and Herbal Extracts Attenuates Intestinal Barrier Dysfunction from Inflammatory Stress

Veronica Cocetta , Daniela Catanzaro , Vittoria Borgonetti , Eugenio Ragazzi , Maria Cecilia Giron , Paolo Governa , Ilaria Carnevali , Marco Biagi ^* , Monica Montopoli ^*

Version 1 : Received: 29 December 2017 / Approved: 29 December 2017 / Online: 29 December 2017 (08:36:36 CET)

How to cite: Cocetta, V.; Catanzaro, D.; Borgonetti, V.; Ragazzi, E.; Giron, M.C.; Governa, P.; Carnevali, I.; Biagi, M.; Montopoli, M. A Fixed Combination of Probiotics and Herbal Extracts Attenuates Intestinal Barrier Dysfunction from Inflammatory Stress. Preprints 2017, 2017120199 (doi: 10.20944/preprints201712.0199.v1). Cocetta, V.; Catanzaro, D.; Borgonetti, V.; Ragazzi, E.; Giron, M.C.; Governa, P.; Carnevali, I.; Biagi, M.; Montopoli, M. A Fixed Combination of Probiotics and Herbal Extracts Attenuates Intestinal Barrier Dysfunction from Inflammatory Stress. Preprints 2017, 2017120199 (doi: 10.20944/preprints201712.0199.v1).

Abstract

Maintenance of intestinal barrier integrity is crucial for preventing inflammatory bowel diseases (IBDs) onset and exacerbations. In this work we study the effect of a fixed combination of Lactobacillus reuteri and Lactobacillus acidophilus and herbal extracts in an in vitro inflammation experimental model. Caco-2 cell monolayer was exposed to INF-γ+TNF-α or to LPS; Trans Epithelial Electrical Resistance (TEER) and paracellular permeability were investigated. ZO-1 and occludin tight junctions (TJs) were also investigated by mean of immunofluorescence. The pre-treatment with the fixed combination of probiotics and herbal extracts prevented the inflammation-induced TEER decrease, paracellular permeability increase and TJs translocation. In summary the fixed combination of probiotics and herbal extracts investigated in this research was found to be an interesting candidate for targeting the re-establishment of intestinal barrier function in IBDs conditions.

Subject Areas

IBD; intestinal barrier; Lactobacillus reuteri; Lactobacillus acidophilus; Trans Epithelial Electrical Resistance (TEER)

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

Your full name

Your affiliation information (e.g., name of your university/institution/organization)

Not displayed online.

Your Email address (not made public)

Please leave your comment to this article below.

Mathematical equations can be typed in either LaTeX formats \\[ ... \\] or $$ ... $$, or MathML format <math> ... </math>. Try the LaTeX or MathML example.

Type equation:

Preview:

Copy equation into message below

Close menu

Please click a symbol to insert it into the message box below:

Please enter the link here:

Optionally, you can enter text that should appear as linked text:

Copy link into message below

Close menu

Please enter or paste the URL to the image here (please only use links to jpg/jpeg, png and gif images):

Copy image into message below

Close menu

Type author name or keywords to filter the list of references in this group (you can add a new citation under Bibliography):

No existing citations in Discussion Group

Wikify editor is a simple editor for wiki-style mark-up. It was written by MDPI for Sciforum in 2014. The rendering of the mark-up is based on Wiky.php with some tweaks. Rendering of mathematical equations is done with MathJax. Please send us a message for support or for reporting bugs.

Close menu

Please declare any conflicts of interest you may have with this paper, financial or otherwise.

I do not have a conflict of interest

I would like to declare a conflict of interest

Display my name on the website I want to get recognition for my comment on Publons

[what’s this?]. Link your accounts here

Captcha

Renew

I accept the following conditions:

Comments must follow the standards of professional discourse and should focus on the scientific content of the article. Insulting or offensive language, personal attacks and off-topic remarks will not be permitted. Comments must be written in English. Preprints reserves the right to remove comments without notice. Readers who post comments are obliged to declare any competing interests, financial or otherwise.

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)

Get PDF Cite Share

Bookmark BibSonomy Mendeley Reddit Delicious Publons

Alerts

Notify me about updates to this article or when a peer-reviewed version is published.