Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Characterization of HIV-1 near Full-Length Proviral Genome Quasispecies from Patients with Undetectable Viral Load Undergoing First-Line HAART Therapy

Brunna M. Alves
,
Juliana D. Siqueira
ORCID logo ,
Marianne M. Garrido
,
Ornella M. Botelho
,
Isabel M. Prellwitz
,
Sayonara R. Ribeiro
,
Esmeralda A. Soares
,
Marcelo A. Soares
* ORCID logo
Version 1 : Received: 1 December 2017 / Approved: 4 December 2017 / Online: 4 December 2017 (09:34:16 CET)

A peer-reviewed article of this Preprint also exists.

Alves, B.M.; Siqueira, J.D.; Garrido, M.M.; Botelho, O.M.; Prellwitz, I.M.; Ribeiro, S.R.; Soares, E.A.; Soares, M.A. Characterization of HIV-1 Near Full-Length Proviral Genome Quasispecies from Patients with Undetectable Viral Load Undergoing First-Line HAART Therapy. Viruses 2017, 9, 392. Alves, B.M.; Siqueira, J.D.; Garrido, M.M.; Botelho, O.M.; Prellwitz, I.M.; Ribeiro, S.R.; Soares, E.A.; Soares, M.A. Characterization of HIV-1 Near Full-Length Proviral Genome Quasispecies from Patients with Undetectable Viral Load Undergoing First-Line HAART Therapy. Viruses 2017, 9, 392.

Abstract

Increased access to highly active antiretroviral therapy (HAART) by HIV+ individuals has become a reality worldwide. In Brazil, ART currently reaches over half of the HIV-infected subjects. In the context of a remarkable HIV-1 genetic variability, highly related variants, called quasispecies, are generated. HIV quasispecies generated during infection can influence virus persistence and pathogenicity, representing a challenge to treatment. However, the clinical relevance of minority quasispecies is still uncertain. For this study, we have determined the archived proviral sequences, viral subtype and drug resistance mutations from a cohort of HIV+ patients with undetectable viral load undergoing HAART as first-line therapy using next-generation sequencing for near full-length virus genome (NFLG) assembly. HIV-1 consensus sequences representing NFLG were obtained for eleven patients, while for another twelve varying genome coverage rates were obtained. Phylogenetic analysis showed the predominance of subtype B (83%; 19/23). Considering the minority variants, 18 patients carried archived virus harboring at least one mutation conferring antiretroviral resistance; for six patients, the mutations correlated with the current ARVs used. These data highlight the importance of monitoring HIV minority drug resistant variants and their clinical impact, to guide future regimen switches and improve HIV treatment success.

Keywords

HIV-1; quasispecies; minority resistance mutations; HAART; drug resistance; undetectable viral load

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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