Preprint Review Version 1 This version not peer reviewed

Impact of Methods on the Measurement of mRNA Turnover

Version 1 : Received: 8 November 2017 / Approved: 9 November 2017 / Online: 9 November 2017 (03:24:17 CET)

A peer-reviewed article of this Preprint also exists.

Wada, T.; Becskei, A. Impact of Methods on the Measurement of mRNA Turnover. Int. J. Mol. Sci. 2017, 18, 2723. Wada, T.; Becskei, A. Impact of Methods on the Measurement of mRNA Turnover. Int. J. Mol. Sci. 2017, 18, 2723.

Journal reference: Int. J. Mol. Sci. 2017, 18, 2723
DOI: 10.3390/ijms18122723

Abstract

The turnover of the RNA molecules is determined by the rates of transcription and RNA degradation. Several methods have been developed to study mRNA turnover since the beginnings of molecular biology. Here we summarize the main methods to measure RNA half-life: transcription inhibition, gene control and metabolic labelling. These methods were used to detect the cellular activity of the mRNAs degradation machinery, including the exo-ribonuclease Xrn1 and the exosome. Less progress has been made in the study of the differential stability of mature RNAs because the different methods have often yielded inconsistent results so that an mRNA considered to be stable can be classified as unstable by another method. Recent advances in the systematic comparison of different method variants in yeast have permitted the identification of the least invasive methodologies that reflect half-lives the most faithfully, which is expected to open the way for a consistent quantitative analysis of the determinants of mRNA stability.

Subject Areas

posttranscriptional regulation; Saccharomyces cerevisiae; nonsense mediated decay; NMD; splicing; 4-thiouracil; 4sU; rpb1-1; exponential decay

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