Obtaining of β-Sitosterol from Cardamom by Supercritical CO2 Extraction

Hacer İçen; Hatice Tuğba Çelik; Mustafa Serhat Ekinci; Metin Gürü

doi:10.20944/preprints201711.0005.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 31 October 2017 / Approved: 1 November 2017 / Online: 1 November 2017 (04:54:35 CET)

Cardamom is plant of the Zingiberaceae family. It has been used for the treatment of many diseases such as migraine, bronchitis, stomach and intestinal disorders. Cardamom contains triterpenes, resins, starch and fatty compounds. Phytosterols (stigmasterol, campesterol and β-sitosterol) are a group steroid alcohol in plants. They are used food, medicine and cosmetic industry. They are protective effects against some types of cancer too. Phytosterols are found in the vegetable oil such as the spindle, corn and soybean oil. This paper deals with the maximum oil and β-sitosterol yield were investigated by means of the supercritical CO₂ extraction of cardamom. The effect of operating parameters as temperature, pressure and CO₂ flow rate were investigated on oil yield. The amount of β-sitosterol was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) at optimized conditions. The optimized conditions were recorded as temperature of 40^oC, pressure of 200 bar and CO₂flow rate of 4 L/min. The maximum oil yield and amount of β-sitosterol were found as 74.83 mg oil/g seed and 4.73 mg β-sitosterol/g seed cardamom under these conditions.

cardamom; supercritical CO2 extraction; β-sitosterol

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Obtaining of β-Sitosterol from Cardamom by Supercritical CO₂ Extraction

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