Obtaining of &beta;-Sitosterol from Cardamom by Supercritical CO2 Extraction

Hacer İçen; Hatice Tuğba Çelik; Mustafa Serhat Ekinci; Metin Gürü

doi:10.20944/preprints201711.0005.v1

Preprint Article Version 1 This version is not peer-reviewed

Obtaining of β-Sitosterol from Cardamom by Supercritical CO₂ Extraction

Hacer İçen ,

Hatice Tuğba Çelik ,

Mustafa Serhat Ekinci ,

Metin Gürü ^*

Version 1 : Received: 31 October 2017 / Approved: 1 November 2017 / Online: 1 November 2017 (04:54:35 CET)

How to cite: İçen, H.; Çelik, H.T.; Ekinci, M.S.; Gürü, M. Obtaining of β-Sitosterol from Cardamom by Supercritical CO₂ Extraction . Preprints 2017, 2017110005 (doi: 10.20944/preprints201711.0005.v1). İçen, H.; Çelik, H.T.; Ekinci, M.S.; Gürü, M. Obtaining of β-Sitosterol from Cardamom by Supercritical CO2 Extraction . Preprints 2017, 2017110005 (doi: 10.20944/preprints201711.0005.v1).

Abstract

Cardamom is plant of the Zingiberaceae family. It has been used for the treatment of many diseases such as migraine, bronchitis, stomach and intestinal disorders. Cardamom contains triterpenes, resins, starch and fatty compounds. Phytosterols (stigmasterol, campesterol and β-sitosterol) are a group steroid alcohol in plants. They are used food, medicine and cosmetic industry. They are protective effects against some types of cancer too. Phytosterols are found in the vegetable oil such as the spindle, corn and soybean oil. This paper deals with the maximum oil and β-sitosterol yield were investigated by means of the supercritical CO₂ extraction of cardamom. The effect of operating parameters as temperature, pressure and CO₂ flow rate were investigated on oil yield. The amount of β-sitosterol was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) at optimized conditions. The optimized conditions were recorded as temperature of 40^oC, pressure of 200 bar and CO₂flow rate of 4 L/min. The maximum oil yield and amount of β-sitosterol were found as 74.83 mg oil/g seed and 4.73 mg β-sitosterol/g seed cardamom under these conditions.

Subject Areas

cardamom; supercritical CO2 extraction; β-sitosterol

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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