Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Anti-Osteoporotic Effects of Kukoamine B in Osteoblast and Osteoclast Cells and Ovariectomized Mice

Eunkuk Park
,
Jeonghyun Kim
,
Mun-Chang Kim
,
Subin Yeo
,
Jieun Kim
,
Chun Whan Choi
,
Hyun-Seok Jin
ORCID logo ,
Sang Woo Lee
,
Wan Yi Li
,
Ji-Won Lee
,
Jin-Hyok Park
,
Dam Huh
* ,
Seon-Yong Jeong
* ORCID logo
Version 1 : Received: 31 October 2017 / Approved: 31 October 2017 / Online: 31 October 2017 (15:35:04 CET)

How to cite: Park, E.; Kim, J.; Kim, M.-C.; Yeo, S.; Kim, J.; Choi, C. W.; Jin, H.-S.; Lee, S. W.; Li, W. Y.; Lee, J.-W.; Park, J.-H.; Huh, D.; Jeong, S.-Y. Anti-Osteoporotic Effects of Kukoamine B in Osteoblast and Osteoclast Cells and Ovariectomized Mice. Preprints 2017, 2017100196. https://doi.org/10.20944/preprints201710.0196.v1 Park, E.; Kim, J.; Kim, M.-C.; Yeo, S.; Kim, J.; Choi, C. W.; Jin, H.-S.; Lee, S. W.; Li, W. Y.; Lee, J.-W.; Park, J.-H.; Huh, D.; Jeong, S.-Y. Anti-Osteoporotic Effects of Kukoamine B in Osteoblast and Osteoclast Cells and Ovariectomized Mice. Preprints 2017, 2017100196. https://doi.org/10.20944/preprints201710.0196.v1

Abstract

Osteoporosis is an abnormal bone remodeling condition characterized by decreased bone density, which leads to high risks of broken bones. Previous studies have demonstrated that Lycii Radicis Cortex (LRC) extract inhibits bone loss in ovariectomized (OVX) mice by enhancing the osteoblast differentiation. A bioactive compound, Kukoamine B (KB), was identified from a fractionation of LRC extract as a candidate component responsible for an anti-osteoporotic effect. This study investigated the anti-osteoporotic effects of KB using in vitro and in vivo osteoporosis models. KB treatment significantly increased the osteoblastic differentiation and mineralized nodule formation of osteoblastic MC3T3-E1 cells, while it significantly decreased the osteoclast differentiation of primary-cultured monocytes derived from mouse bone marrow. The effects of KB on osteoblastic and osteoclastic differentiations under more physiological conditions were also examined. In the co-culture of MC3T3-E1 cells and monocytes, KB promoted osteoblast differentiation but did not affect osteoclast differentiation. For the in vivo experiments, KB significantly inhibited OVX-induced bone mineral density loss and restored the impaired bone structural properties in osteoporosis model mice. These results suggest that KB may be a potential therapeutic candidate for the treatment of osteoporosis.

Keywords

osteoporosis; herbal medicine; Kukoamine B; osteoblast; osteoclast; bone mineral density; ovariectomized mice

Subject

Chemistry and Materials Science, Medicinal Chemistry

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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