Preprint Article Version 1 This version is not peer-reviewed

Molecular Cloning, Expression and Functional Analysis of Interferon Regulatory Factor 5 (IRF 5) in Malabar Grouper (Epinephelus Malabaricus)

Version 1 : Received: 20 September 2017 / Approved: 22 September 2017 / Online: 22 September 2017 (08:58:20 CEST)

How to cite: Periyasamy, T.; Lu, M. Molecular Cloning, Expression and Functional Analysis of Interferon Regulatory Factor 5 (IRF 5) in Malabar Grouper (Epinephelus Malabaricus). Preprints 2017, 2017090106 (doi: 10.20944/preprints201709.0106.v1). Periyasamy, T.; Lu, M. Molecular Cloning, Expression and Functional Analysis of Interferon Regulatory Factor 5 (IRF 5) in Malabar Grouper (Epinephelus Malabaricus). Preprints 2017, 2017090106 (doi: 10.20944/preprints201709.0106.v1).

Abstract

Interferon regulatory factor 5 (IRF5) is known to be involved in the innate immune response and pro-inflammatory cytokines. However, the roles of IRF5 in immune responses in Malabar grouper (Epinephelus malabaricus) have not been extensively explored. In this study, IRF5 gene was identified and characterized from M. grouper. The full-length IRF5 cDNA consisted of a 5’ terminal untranslated region (5’-UTR) of 289 bp and a 3’-UTR of 542 bp, an open reading frame (ORF) of 1500 bp encoding a polypeptide of 499 amino acids with a predicted molecular mass of 56.28 kDa and isoelectric point (pI) of 5.2. The putative MgIRF5 protein consists of four important conserved domains: a helix DNA-binding domain (DBD) at the N-terminus, a middle region, an IRF association domain (IAD) and a virus activated domain (VAD) at the C-terminus. Sequence alignment and phylogenetic analysis showed that highest sequence similarity of IRF5 was observed between the IRF5 genes from Oplegnathus fasciatus and Miichthys miiuy. The mRNA transcripts of IRF5 were detected in a wide range of tissues types from healthy M. grouper with highest expression in muscle, liver and skin. After treatment with poly (I: C), it was significantly up-regulated in spleen and liver tissues. When infected with NNV, the expression level of MgIRF5 was up-regulated in spleen and head kidney and their transcriptional responses to IRF5 increased in the grouper kidney cells. This approach suggests that MgIRF5 is important in the underlying mechanism of the innate immune responses against antiviral response.

Subject Areas

IRF5; transcriptional factors; immuno-stimulation; nervous necrosis virus; malabar grouper (epinephelus malabaricus)

Readers' Comments and Ratings (0)

Leave a public comment
Send a private comment to the author(s)
Rate this article
Views 0
Downloads 0
Comments 0
Metrics 0
Leave a public comment

×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.