Preprint Article Version 1 This version is not peer-reviewed

Glycemic Regulation and Cytotoxic Characteristics of the Ethyl Acetate Fraction from Passiflora cincinnata Mast Leaves

Version 1 : Received: 17 August 2017 / Approved: 17 August 2017 / Online: 17 August 2017 (10:29:55 CEST)

How to cite: Estrevam, C.D.S.; Lima, C.A.; Santos, J.L.D.; Marchioro, M.; Araujo, B.S.; Corrêa, C.B.; Santos, C.M.; Santana, I.S.; Araújo, S.S. Glycemic Regulation and Cytotoxic Characteristics of the Ethyl Acetate Fraction from Passiflora cincinnata Mast Leaves. Preprints 2017, 2017080060 (doi: 10.20944/preprints201708.0060.v1). Estrevam, C.D.S.; Lima, C.A.; Santos, J.L.D.; Marchioro, M.; Araujo, B.S.; Corrêa, C.B.; Santos, C.M.; Santana, I.S.; Araújo, S.S. Glycemic Regulation and Cytotoxic Characteristics of the Ethyl Acetate Fraction from Passiflora cincinnata Mast Leaves. Preprints 2017, 2017080060 (doi: 10.20944/preprints201708.0060.v1).

Abstract

Passiflora cincinnata Mast is a plant popularly used in traditional medicine in northeastern Brazil. The present study aimed to evaluate the anti-lipoperoxidative and anti-α-amylase properties, and cytotoxicity of an extract and fraction of passion fruit leaves (P. cincinnata Mast), as well as the antihyperglecemiant activity of the fraction rich in glycosylated flavonoids and showing low cytotoxicity in rats with a postprandial hyperglycaemia condition. The ethyl acetate fraction (F.ACT) of the P. cincinnata leaves presented the best anti-lipoperoxide properties with TBARs 81.49%, 95.48% and 75.62% lower than AAPH, FeSO4 and H2O2 induced controls, respectively, at 200 μg.mL-1. In addition, it presented an anti-α-amylase potential, with a better ability to inhibit the α-amylase enzyme in comparison to the acarbose control (IC50 6.49 ± 0.11 and 12.01 ± 0.4 μg.mL-1, respectively). The hydroalcoholic extract of P. cincinnata (ExEt) presented high content of total tannins, flavonoids and flavonols. However, F.ACT had the highest concentration of flavonoids among the fractions studied. HPLC analysis of this fraction revealed the presence of the flavonoids isovitexin, orientin and isoorientin. F.ACT showed low to no cytotoxicity below 150 μg.mL-1. Regarding the post-prandial antihyperglycaemic activity of F.ACT, it was observed at 50 and 100 mg.kg-1.

Subject Areas

Passiflora cincinnata Mast; redox activity; cell cytotoxicity; α-amylase inhibition; antihyperglycaemic

Readers' Comments and Ratings (0)

Leave a public comment
Send a private comment to the author(s)
Rate this article
Views 0
Downloads 0
Comments 0
Metrics 0
Leave a public comment

×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.