PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Detection of Aβ(1-40) Protein in Human Serum as A Causative Agent of Alzheimer’s Disease by Strain Gauge Cantilever Biosensor Immobilizing Liposome Incorporating Cholesterol
Version 1
: Received: 8 August 2017 / Approved: 8 August 2017 / Online: 8 August 2017 (09:57:28 CEST)
How to cite:
Taniguchi, T.; Murakami, Y.; Sohgawa, M.; Yamashita, K.; Noda, M. Detection of Aβ(1-40) Protein in Human Serum as A Causative Agent of Alzheimer’s Disease by Strain Gauge Cantilever Biosensor Immobilizing Liposome Incorporating Cholesterol. Preprints2017, 2017080031. https://doi.org/10.20944/preprints201708.0031.v1.
Taniguchi, T.; Murakami, Y.; Sohgawa, M.; Yamashita, K.; Noda, M. Detection of Aβ(1-40) Protein in Human Serum as A Causative Agent of Alzheimer’s Disease by Strain Gauge Cantilever Biosensor Immobilizing Liposome Incorporating Cholesterol. Preprints 2017, 2017080031. https://doi.org/10.20944/preprints201708.0031.v1.
Cite as:
Taniguchi, T.; Murakami, Y.; Sohgawa, M.; Yamashita, K.; Noda, M. Detection of Aβ(1-40) Protein in Human Serum as A Causative Agent of Alzheimer’s Disease by Strain Gauge Cantilever Biosensor Immobilizing Liposome Incorporating Cholesterol. Preprints2017, 2017080031. https://doi.org/10.20944/preprints201708.0031.v1.
Taniguchi, T.; Murakami, Y.; Sohgawa, M.; Yamashita, K.; Noda, M. Detection of Aβ(1-40) Protein in Human Serum as A Causative Agent of Alzheimer’s Disease by Strain Gauge Cantilever Biosensor Immobilizing Liposome Incorporating Cholesterol. Preprints 2017, 2017080031. https://doi.org/10.20944/preprints201708.0031.v1.
Abstract
We have successfully measured amyloid beta (Aβ) (1-40) protein added in human serum by a NiCr strain gauge cantilever biosensor immobilized with liposomes incorporating cholesterol. Importantly, we investigated the effect of incorporation of cholesterol in the liposome in order to suppress the interaction between the liposome and many different proteins included in human serum. It was revealed that incorporating cholesterol suppresses the interaction between the proteins other than Aβ in human serum and the liposome. Finally, we detected Aβ(1-40) in human serum with typical chronological behaviors due to Aβ aggregation and fibrillization. Furthermore, as a digital low-pass filtering procedure could reduce external noises, the cantilever sensor immobilized with liposome incorporating cholesterol can detect low-concentrated Aβ in human serum.
Keywords
cantilever; NiCr strain gauge; biosensor; liposome; amyloid beta; aggregation; fibrillization; interaction; human serum; digital low-pass filtering procedure
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
