Insulin Secretion with the Effect of Loureirin B through KATP Channel–Dependent Pathway

Yijie Sha; Yuelin Zhang; Jing Cao; Kai Qian; Bing Niu; Qin Chen

doi:10.20944/preprints201705.0189.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 25 May 2017 / Approved: 26 May 2017 / Online: 26 May 2017 (04:52:12 CEST)

The development of new diabetes drugs continues to be explored. Loureirin B, a flavonoid, extracted from Dracaena cochinchinensis, has been confirmed to increase insulin secretion and decrease blood glucose levels. For understanding the mechanism, a series of experiments had been employed based on computational methods and cell experiments. The insulin secretion significantly increased with the incubation of 0.01μM loureirin B for 4 hours. The viability of Ins-1 cells showed no significant difference with the treatment of loureirin B. Through computational methods, we hypothesized that loureirin B could interacts with K_ATP channels to promote insulin secretion. In cell experiments, it could be found that the current of K_ATP channel of Ins-1 cells was inhibited by the effect of loureirin B. After then, the voltage-dependent calcium channels were activated, the increase of Cx43 protein expression might mediate the Ca²⁺ to the intracellular. In summary, it could be concluded that loureirin B promoted insulin secretion mainly through inhibiting K_ATP current, the influx of Ca²⁺ to the Intracellular and the expression of Cx43.

loureirin B; Ins-1 cells; Insulin secretion; KATP channel; influx of Ca2+; expression of Cx43

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Insulin Secretion with the Effect of Loureirin B through K_ATPChannel–Dependent Pathway

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