Version 1
: Received: 25 May 2017 / Approved: 26 May 2017 / Online: 26 May 2017 (04:52:12 CEST)
How to cite:
Sha, Y.; Zhang, Y.; Cao, J.; Qian, K.; Niu, B.; Chen, Q. Insulin Secretion with the Effect of Loureirin B through KATP Channel–Dependent Pathway. Preprints2017, 2017050189 (doi: 10.20944/preprints201705.0189.v1).
Sha, Y.; Zhang, Y.; Cao, J.; Qian, K.; Niu, B.; Chen, Q. Insulin Secretion with the Effect of Loureirin B through KATP Channel–Dependent Pathway. Preprints 2017, 2017050189 (doi: 10.20944/preprints201705.0189.v1).
Cite as:
Sha, Y.; Zhang, Y.; Cao, J.; Qian, K.; Niu, B.; Chen, Q. Insulin Secretion with the Effect of Loureirin B through KATP Channel–Dependent Pathway. Preprints2017, 2017050189 (doi: 10.20944/preprints201705.0189.v1).
Sha, Y.; Zhang, Y.; Cao, J.; Qian, K.; Niu, B.; Chen, Q. Insulin Secretion with the Effect of Loureirin B through KATP Channel–Dependent Pathway. Preprints 2017, 2017050189 (doi: 10.20944/preprints201705.0189.v1).
Abstract
The development of new diabetes drugs continues to be explored. Loureirin B, a flavonoid, extracted from Dracaena cochinchinensis, has been confirmed to increase insulin secretion and decrease blood glucose levels. For understanding the mechanism, a series of experiments had been employed based on computational methods and cell experiments. The insulin secretion significantly increased with the incubation of 0.01μM loureirin B for 4 hours. The viability of Ins-1 cells showed no significant difference with the treatment of loureirin B. Through computational methods, we hypothesized that loureirin B could interacts with KATP channels to promote insulin secretion. In cell experiments, it could be found that the current of KATP channel of Ins-1 cells was inhibited by the effect of loureirin B. After then, the voltage-dependent calcium channels were activated, the increase of Cx43 protein expression might mediate the Ca2+ to the intracellular. In summary, it could be concluded that loureirin B promoted insulin secretion mainly through inhibiting KATP current, the influx of Ca2+ to the Intracellular and the expression of Cx43.
Subject Areas
loureirin B; Ins-1 cells; Insulin secretion; KATP channel; influx of Ca2+; expression of Cx43
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Yijie Sha