Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Inhibitory Effects of Diclofenac on Steroid Glucuronidation in Vivo Do not Affect Hair-Based Doping Tests for Stanozolol

Gergely Zachár
ORCID logo ,
Nawed I. K. Deshmukh
,
Andrea Petróczi
,
Andrea D. Székely
,
Iltaf Shah
ORCID logo ,
James Barker
ORCID logo ,
Declan P. Naughton
*
Version 1 : Received: 23 May 2017 / Approved: 24 May 2017 / Online: 24 May 2017 (08:25:26 CEST)

A peer-reviewed article of this Preprint also exists.

Zachár, G.; Deshmukh, N.I.; Petróczi, A.; Székely, A.D.; Shah, I.; Barker, J.; Naughton, D.P. Inhibitory Effects of Diclofenac on Steroid Glucuronidation In Vivo Do Not Affect Hair-Based Doping Tests for Stanozolol. Molecules 2017, 22, 976. Zachár, G.; Deshmukh, N.I.; Petróczi, A.; Székely, A.D.; Shah, I.; Barker, J.; Naughton, D.P. Inhibitory Effects of Diclofenac on Steroid Glucuronidation In Vivo Do Not Affect Hair-Based Doping Tests for Stanozolol. Molecules 2017, 22, 976.

Abstract

In vitro studies show that diclofenac inhibits enzymatic steroid glucuronidation. This study was designed to investigate the influence of diclofenac on the excretion of stanozolol and 3'-hydroxystanozolol via analyses in hair, blood and urine in vivo in a rat study. Brown Norway rats were administered with stanozolol (weeks 1-3) and diclofenac (weeks 1-6). Weekly assessment of steroid levels in hair was complemented with spot urine and serum tests. Levels of both stanozolol and 3'-hydroxystanozolol steadily increased in hair during stanozolol treatment and decreased post-treatment, but remained readily detectable for 6 weeks. In contrast, compared to control rats, diclofenac significantly reduced urinary excretion of 3’-hydroxystanozolol which was undetectable in most samples. This is the first report of diclofenac altering steroid metabolism in vivo, detrimentally affecting detection in urine, but not in hair which holds considerable advantages over urinalysis for anti-doping tests.

Keywords

steroid; metabolism; anti-inflammatory drug; inhibition

Subject

Biology and Life Sciences, Endocrinology and Metabolism

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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