Preprint Article Version 1 NOT YET PEER-REVIEWED

Melanin Bleaching with Warm Hydrogen Peroxide and Integrated Immunohistochemical Analysis: An Automated Platform 

Version 1 : Received: 20 March 2017 / Approved: 21 March 2017 / Online: 21 March 2017 (04:23:40 CET)

How to cite: Liu, C.; Lin, C.; Tsai, M.; Chen, Y.; Yang, S.; Tsai, K. Melanin Bleaching with Warm Hydrogen Peroxide and Integrated Immunohistochemical Analysis: An Automated Platform . Preprints 2017, 2017030167 (doi: 10.20944/preprints201703.0167.v1). Liu, C.; Lin, C.; Tsai, M.; Chen, Y.; Yang, S.; Tsai, K. Melanin Bleaching with Warm Hydrogen Peroxide and Integrated Immunohistochemical Analysis: An Automated Platform . Preprints 2017, 2017030167 (doi: 10.20944/preprints201703.0167.v1).

Abstract

Diagnosing melanocytic lesions is among the most challenging problems in the practice of pathology. The difficulty of physically masking melanin pigment and the similarity of its color to commonly used chromogens often complicate examination of the cytomorphology and immunohistochemical staining results for tumor cells. Melanin bleach can be very helpful for histopathological diagnosis of heavily pigmented melanocytic lesions. Although various depigmentation methods have been reported, no standardized methods have been developed. This study developed a fully automated platform that incorporates hydrogen peroxide-based melanin depigmentation in an automated immunohistochemical analysis. The utility of the method was tested in one cell block of malignant melanoma cells in pleural effusion, ten ocular melanoma tissue samples, and ten cutaneous melanoma tissue samples. Our results demonstrated that the proposed method, which can be performed in only 3 hours, effectively preserves cell cytomorphology and immunoreactivity. The method is particularly effective for removing melanin pigment to facilitate histopathological examination of cytomorphology and for obtaining an unmasked tissue section for immunohistochemical analysis.

Subject Areas

melanin bleaching; immunohistochemistry; automated platform

Readers' Comments and Ratings (0)

Leave a public comment
Send a private comment to the author(s)
Rate this article
Views 0
Downloads 0
Comments 0
Metrics 0
Leave a public comment

×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.