Preprint Technical Note Version 1 NOT YET PEER-REVIEWED

An Alternative Method to Niskin Sampling for More Accurate Metatranscriptomic Analysis of the Marine Environment

Version 1 : Received: 9 March 2017 / Approved: 10 March 2017 / Online: 10 March 2017 (10:50:15 CET)

How to cite: Teague, J.; Scott, T.; Sharma, S.; Graham, G.; Allen, M. An Alternative Method to Niskin Sampling for More Accurate Metatranscriptomic Analysis of the Marine Environment. Preprints 2017, 2017030057 (doi: 10.20944/preprints201703.0057.v1). Teague, J.; Scott, T.; Sharma, S.; Graham, G.; Allen, M. An Alternative Method to Niskin Sampling for More Accurate Metatranscriptomic Analysis of the Marine Environment. Preprints 2017, 2017030057 (doi: 10.20944/preprints201703.0057.v1).

Abstract

The development of low-cost, open-source Remotely Operated Vehicle (ROV) systems has provided almost unrestricted access for researchers looking to monitor the marine environment in ever greater resolution. Sampling microbial communities from the marine environment, however, still usually relies on Niskin-bottle sampling (ROV or CTD based), a method which introduces an inaccuracy and variability that is incompatible with metatranscriptomic analysis. Here, we describe a versatile, easily-replicated platform which achieves in situ mRNA preservation, via the addition of RNAlater to filtered microbial cells, to enhance ROV or CTD functionality.

Subject Areas

Remotely Operated Vehicle; Metatranscriptomics; Niskin

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