Preprint Technical Note Version 1 Preserved in Portico This version is not peer-reviewed

An Alternative Method to Niskin Sampling for More Accurate Metatranscriptomic Analysis of the Marine Environment

Version 1 : Received: 9 March 2017 / Approved: 10 March 2017 / Online: 10 March 2017 (10:50:15 CET)

A peer-reviewed article of this Preprint also exists.

Teague, J.; Scott, T.B.; Sharma, S.; Graham, G.; Allen, M.J. An Alternative Method to Niskin Sampling for Molecular Analysis of the Marine Environment. J. Mar. Sci. Eng. 2017, 5, 22. Teague, J.; Scott, T.B.; Sharma, S.; Graham, G.; Allen, M.J. An Alternative Method to Niskin Sampling for Molecular Analysis of the Marine Environment. J. Mar. Sci. Eng. 2017, 5, 22.

Abstract

The development of low-cost, open-source Remotely Operated Vehicle (ROV) systems has provided almost unrestricted access for researchers looking to monitor the marine environment in ever greater resolution. Sampling microbial communities from the marine environment, however, still usually relies on Niskin-bottle sampling (ROV or CTD based), a method which introduces an inaccuracy and variability that is incompatible with metatranscriptomic analysis. Here, we describe a versatile, easily-replicated platform which achieves in situ mRNA preservation, via the addition of RNAlater to filtered microbial cells, to enhance ROV or CTD functionality.

Keywords

Remotely Operated Vehicle; Metatranscriptomics; Niskin

Subject

Biology and Life Sciences, Immunology and Microbiology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.