Immunization with E Plus NS1-2a Enhanced Protection against Dengue Virus Serotype 2 in Mice

Yanhua Wu; Shuyu Fang; Xiaoyun Cui; Na Gao; Dongying Fan; Jing An

doi:10.20944/preprints201612.0006.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 1 December 2016 / Approved: 1 December 2016 / Online: 1 December 2016 (10:37:10 CET)

Dengue virus (DENV), the causative agent of dengue fever (DF), is one of the most important mosquito-borne viruses that can infect humans. Although much effort has been made on prevention and control of dengue, there are currently no anti-viral drugs or worldwide approved vaccines yet. In this study, we immunized six-week-old Balb/c mice with DNA vaccine candidates E and NS1-2a of DENV serotype 2 or the combination of them (E+NS1-2a) via an electroporation (EP)-assisted intramuscular gene delivery system and evaluated the immune response and protection. The highest specific antibody titres and cytokine levels secreted by splenocytes as well as the highest survival rate were observed in the E+NS1-2a group, followed by E group and NS1-2a group. Our data suggested that the combination of E and NS1-2a delivered by EP may be a superior preventive strategy against DENV.

Dengue virus; E; NS1-2a; electroporation; DNA vaccine

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Immunization with E Plus NS1-2a Enhanced Protection against Dengue Virus Serotype 2 in Mice

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