Systematic sampling on real line (R) when using the different probes is very attractive method within which the biomedical imaging is consulted by a surgery, etc. This study is an extension of [16], and an exact calculation method is proposed for the calculation of constant λq of confidence interval for the systematic sampling. If the smoothness constant q of measurement function occurred by slicing the three dimensional object is estimated to be enough small mean square error, we can make the important remarks for the design-based stereology used as a method to get the quantitative results from the tissues and the radiological images. Synthetic data can support the results of real data. The currently used covariogram model proposed by [28] is tested for the different measurement functions to see the performance on the variance estimation. The exact value of constant λq is examined for the different measurement functions as well.