Preprint Opinion Version 1 NOT YET PEER-REVIEWED

Dark Side of Molecular Techniques: How to Fix It ?

Version 1 : Received: 2 August 2016 / Approved: 2 August 2016 / Online: 2 August 2016 (10:42:27 CEST)

How to cite: Bhardwaj, V. Dark Side of Molecular Techniques: How to Fix It ?. Preprints 2016, 2016080016 (doi: 10.20944/preprints201608.0016.v1). Bhardwaj, V. Dark Side of Molecular Techniques: How to Fix It ?. Preprints 2016, 2016080016 (doi: 10.20944/preprints201608.0016.v1).

Abstract

Many fundamental molecular techniques (PCR, Microarray, Southern and northern hybridization, siRNA, CRISPR/Cas9 etc.) developed so far shows errors. I wish to highlight these molecular techniques are developed on basis of Watson-Crick DNA model, ignoring the concept of parallel stranded DNA. Through this opinion article, I wish to highlight specificity and accuracy of these molecular techniques can be enhanced by considering both parallel and anti parallel hybridization of DNA. Hopefully my views will also solve issue of irreproducibility in life science research.

Subject Areas

parallel DNA; antiparallel DNA; PCR; CRISPR; nucleic acid hybridization; microarray; siRNA

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