Opinion
Version 1
Preserved in Portico This version is not peer-reviewed
Dark Side of Molecular Techniques: How to Fix It ?
Version 1
: Received: 2 August 2016 / Approved: 2 August 2016 / Online: 2 August 2016 (10:42:27 CEST)
How to cite: Bhardwaj, V. Dark Side of Molecular Techniques: How to Fix It ?. Preprints 2016, 2016080016. https://doi.org/10.20944/preprints201608.0016.v1 Bhardwaj, V. Dark Side of Molecular Techniques: How to Fix It ?. Preprints 2016, 2016080016. https://doi.org/10.20944/preprints201608.0016.v1
Abstract
Many fundamental molecular techniques (PCR, Microarray, Southern and northern hybridization, siRNA, CRISPR/Cas9 etc.) developed so far shows errors. I wish to highlight these molecular techniques are developed on basis of Watson-Crick DNA model, ignoring the concept of parallel stranded DNA. Through this opinion article, I wish to highlight specificity and accuracy of these molecular techniques can be enhanced by considering both parallel and anti parallel hybridization of DNA. Hopefully my views will also solve issue of irreproducibility in life science research.
Keywords
parallel DNA; antiparallel DNA; PCR; CRISPR; nucleic acid hybridization; microarray; siRNA
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Comments (0)
We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.
Leave a public commentSend a private comment to the author(s)
* All users must log in before leaving a comment
