Imidacloprid is the first-generation neonicotinoid insecticide. But, the long-term use of im-idacloprid as a pesticide has caused severe water pollution. Recently, the toxicity of imidacloprid to aquatic organisms has received increasing attention. This study aimed to investigate the ab-sorption and distribution of imidacloprid in various tissues (gills, intestine, liver, muscle, brain, and gonads) of goldfish through short-term and continuous exposure tests for 28 days. The re-sults of short-term exposure indicated that the concentration of imidacloprid and its metabolites in tissues at the transfer stage decreased steadily after 1 day of 40 mg/l imidacloprid water treatment and was below the detection limit after 3 days. Continuous exposure for 28 days at various treatment concentrations showed that the concentrations of imidacloprid and its metab-olites differed significantly among the different tissues of the goldfish. In the 20 mg/l treatment group (S1), the highest concentration of imidacloprid was found in the liver (12.04 μg /gtissue), followed by the intestine (9.91 μg /gtissue), muscle (6.20 μg /gtissue), gill (6.11 μg /gtissue), gonads (5.22 μg /gtissue), and brain (2.87 μg /gtissue). In the 40 mg/l treatment group (S2), the order of tissue concentrations was similar to that of the S1 group, with the highest concentration observed in the liver (12.04 μg/gtissue), followed by the intestine (9.91 μg/gtissue), muscle (6.20 μg/gtissue), gill (6.11 μg/gtissue), gonads (5.22 μg/gtissue), and brain (2.87 μg/gtissue). Fur-thermore, the study detected 5-hydroxyimidacloprid, imidacloprid urea, and 6-chloronicotinic acid in imidacloprid metabolites in all tissues, while imidacloprid was detected only in the intes-tine and liver. Overall, the results of this study contribute to a better understanding of the met-abolic behavior of imidacloprid in organisms and provide new data to support the assessment of imidacloprid toxicity in fish.