Submitted:
06 August 2026
Posted:
13 August 2026
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Abstract
Keywords:
Background
Methods
Results
| Sensitivity family | Status | Result or decision |
|---|---|---|
| Calculated versus source-reported threshold date | Not feasible | Zone-specific threshold-completion dates were not reported consistently. The prespecified calculated date was retained. |
| National versus World Health Organization recurrence date | Completed | Masereka: 3 July versus 5 July; no material effect on conclusions. |
| All episodes versus first episode per zone | Structurally identical | Each zone contributed one episode; no repeat episodes were verified. |
| Exclusion below high episode-validity confidence | Not applicable | All 17 episodes had high episode-validity confidence. |
| Administrative follow-up restriction | Completed | Recurrence proportions were 66.7%, 71.4% and 66.7% at ≥7, ≥14 and ≥21 days of potential observation. |
| Alternative spatial definitions | Completed | Primary adjacency was unchanged by 1 km tolerance and equal-area reprojection; centroid definitions were less coherent for large zones. |
| Exclusion for laboratory or reporting disruption | Not feasible | Indicators were not reported consistently across all episodes; no selective post hoc exclusion was applied. |

Discussion
Limitations
Conclusions
List of Abbreviations
Author Contributions
Funding
Ethics Approval and Consent to Participate
Consent for Publication
Availability of Data and Materials
Acknowledgments
Competing Interests
References
- Towner, J.S.; Sealy, T.K.; Khristova, M.L.; Albariño, C.G.; Conlan, S.; Reeder, S.A.; et al. Newly Discovered Ebola Virus Associated with Hemorrhagic Fever Outbreak in Uganda. PLoS Pathog.;PubMed 2008, 4(11), e1000212. [Google Scholar] [CrossRef] [PubMed]
- MacNeil, A.; Farnon, E.C.; Wamala, J.; Okware, S.; Cannon, D.L.; Reed, Z.; et al. Proportion of Deaths and Clinical Features in Bundibugyo Ebola Virus Infection, Uganda. Emerg. Infect. Dis.;PubMed 2010, 16(12), 1969–72. [Google Scholar] [CrossRef] [PubMed]
- Kratz, T.; Roddy, P.; Oloma, A.T.; Jeffs, B.; Ciruelo, D.P.; De La Rosa, O.; et al. Ebola Virus Disease Outbreak in Isiro, Democratic Republic of the Congo, 2012: Signs and Symptoms, Management and Outcomes. PLoS ONE;PubMed 2015, 10(6), e0129333. [Google Scholar] [CrossRef] [PubMed]
- Ministère de la Santé Publique H et PS. Déclaration de la 17e épidémie de la maladie à virus Ebola dans les zones de santé de Rwampara, Mongbwalu et Bunia dans la province de l’Ituri, République Démocratique du Congo; Kinshasa, May 2026. [Google Scholar]
- World Health Organization. Ebola disease caused by Bundibugyo virus - Democratic Republic of the Congo [Internet]. 2026. Available online: https://www.who.int/emergencies/disease-outbreak-news/item/2026-DON614.
- World Health Organization. WHO recommended criteria for declaring the end of the Ebola virus disease outbreak [Internet]. Geneva, Mar 2020. Available online: https://www.who.int/publications/m/item/who-recommended-criteria-for-declaring-the-end-of-the-ebola-virus-disease-outbreak.
- World Health Organization. Ebola disease caused by Bundibugyo virus, Democratic Republic of the Congo & Uganda [Internet]. 2026. Available online: https://www.who.int/emergencies/disease-outbreak-news/item/2026-DON613.
- Thompson, R.N.; Morgan, O.W.; Jalava, K. Rigorous surveillance is necessary for high confidence in end-of-outbreak declarations for Ebola and other infectious diseases. Philos. Trans. R. Soc. B Biol. Sci.;PubMed 2019, 374(1776), 20180431. [Google Scholar] [CrossRef] [PubMed]
- Mccabe, R.; Ebbarnezh, L.; Okware, S.; Fotsing, R.; Koua, E.; Mbaka, P.; et al. Estimation of the size of the outbreak of Ebola disease caused by Bundibugyo virus in the Democratic Republic of the Congo; Imperial College London, 18 May 2026. [Google Scholar] [CrossRef] [PubMed]
- Centre des Operations d Urgences de Sante Publique RDC. Rapport de situation de la 17eme epidemie de la maladie a virus Ebola RDC [Internet]. 2026. Available online: https://insp.cd/sitrep-mve-n-013-2026/.
- Keita, A.K.; Koundouno, F.R.; Faye, M.; Düx, A.; Hinzmann, J.; Diallo, H.; et al. Resurgence of Ebola virus in 2021 in Guinea suggests a new paradigm for outbreaks. Nature;PubMed 2021, 597(7877), 539–43. [Google Scholar] [CrossRef] [PubMed]
- Mbala-Kingebeni, P.; Pratt, C.; Mutafali-Ruffin, M.; Pauthner, M.G.; Bile, F.; Nkuba-Ndaye, A.; et al. Ebola Virus Transmission Initiated by Relapse of Systemic Ebola Virus Disease. N. Engl. J. Med.;PubMed 2021, 384(13), 1240–7. [Google Scholar] [CrossRef] [PubMed]
- Diallo, B.; Sissoko, D.; Loman, N.J.; Bah, H.A.; Bah, H.; Worrell, M.C.; et al. Resurgence of Ebola Virus Disease in Guinea Linked to a Survivor With Virus Persistence in Seminal Fluid for More Than 500 Days. Clin. Infect. Dis.;PubMed 2016, 63(10), 1353–6. [Google Scholar] [CrossRef] [PubMed]
- Merci Amuda, D.; Araali, B.; Agenonga Chober, A.; Bedford, J.; Boone, D.; Duclos, D.; et al. Note contextuelle sur l’épidémie d’Ebola Bundibugyo en Ituri (2026). HAL Open Sci. 2026, (1), 255. [Google Scholar] [CrossRef]
- World Health Organization. EBOLA BUNDIBUGYO VIRUS DISEASE OUTBREAK Democratic Republic of the Congo | Uganda Weekly External Situation Report 05, Data as of 14 June 2026 | WHO | Regional Office for Africa [Internet]. 2026. Available online: https://www.afro.who.int/countries/uganda/publication/ebola-bundibugyo-virus-disease-outbreak-democratic-republic-congo-1.
- GRID3. Geospatial data DRC - GRID3 [Internet]. 28 Jul 2026. Available online: https://grid3.org/geospatial-data-drc.
- Von Elm, E.; Altman, D.G.; Egger, M.; Pocock, S.J.; Gøtzsche, P.C.; Vandenbroucke, J.P. The Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) Statement: Guidelines for Reporting Observational Studies. PLoS Med.;PubMed 2007, 4(10), e296. [Google Scholar] [CrossRef] [PubMed]
- Keita, M.; Polonsky, J.; Finci, I.; Mbala-Kingebeni, P.; Ilumbulumbu, M.K.; Dakissaga, A.; et al. Investigation of and Strategies to Control the Final Cluster of the 2018-2020 Ebola Virus Disease Outbreak in the Eastern Democratic Republic of Congo. Open Forum Infect. Dis. 2022, 9(9). [Google Scholar] [CrossRef] [PubMed]
- Backer, J.A.; Wallinga, J.; Lessler, J.; Hopkins Bloomberg, J. Spatiotemporal Analysis of the 2014 Ebola Epidemic in West Africa. PLoS Comput Biol. 2016, 12(12), e1005210. [Google Scholar] [CrossRef] [PubMed]



| Panel / estimand | Estimate or timepoint | Number at risk | 95% confidence interval or detail |
|---|---|---|---|
| Panel A: primary estimates | Observed recurrence: 10/17 (58.8%) | — | 32.9% to 81.6% |
| Zone-days at risk: 353 | — | Threshold to recurrence or censoring | |
| Incidence: 2.83 per 100 zone-days | — | 1.36 to 5.21 | |
| Median among recurrent zones: 13.5 days | — | Recurrent zones only | |
| Kaplan–Meier median: 22 days | — | All episodes; censoring-adjusted | |
| Panel B: cumulative recurrence | Day 7: 20.0% | 12 | 6.9% to 50.0% |
| Day 14: 41.3% | 9 | 21.0% to 70.0% | |
| Day 21: 48.7% | 7 | 26.8% to 76.0% | |
| Day 28: 56.0% | 6 | 32.9% to 81.5% |
| Health zone | Last verified activity | Threshold date | Outcome date and status | Interval, days | Recurrence-date confidence |
|---|---|---|---|---|---|
| Miti-Murhesa | 20 May | 10 June | 2 August; censored | 53 | Not applicable |
| Gety | 21 May | 11 June | 2 August; censored | 52 | Not applicable |
| Kalunguta | 24 May | 14 June | 26 July; recurrence | 42 | High |
| Goma | 25 May | 15 June | 2 August; censored | 48 | Not applicable |
| Mambasa | 1 June | 22 June | 6 July; recurrence | 14 | High |
| Aru | 4 June | 25 June | 17 July; recurrence | 22 | High |
| Rimba | 4 June | 25 June | 5 July; recurrence | 10 | High |
| Masereka | 10 June | 1 July | 3 July; recurrence | 2 | Moderate (3 vs 5 July) |
| Vuhovi | 10 June | 1 July | 2 August; recurrence | 32 | High |
| Mabalako | 13 June | 4 July | 2 August; censored | 29 | Not applicable |
| Aungba | 18 June | 9 July | 26 July; recurrence | 17 | High |
| Oicha | 18 June | 9 July | 22 July; recurrence | 13 | High |
| Kilo | 24 June | 15 July | 17 July; recurrence | 2 | High |
| Logo | 25 June | 16 July | 19 July; recurrence | 3 | High |
| Kambala | 30 June | 21 July | 2 August; censored | 12 | Not applicable |
| Lubunga | 11 July | 1 August | 2 August; censored | 1 | Not applicable |
| Mangobo | 11 July | 1 August | 2 August; censored | 1 | Not applicable |
| Health zone | Outcome | Adjacent active zone | Number of active adjacent zones | Minimum boundary distance, km |
|---|---|---|---|---|
| Aru | Recurrence | Yes | 1 | 0.0 |
| Aungba | Recurrence | Yes | 2 | 0.0 |
| Kalunguta | Recurrence | Yes | 2 | 0.0 |
| Kilo | Recurrence | Yes | 3 | 0.0 |
| Logo | Recurrence | Yes | 1 | 0.0 |
| Mambasa | Recurrence | Yes | 2 | 0.0 |
| Masereka | Recurrence | Yes | 2 | 0.0 |
| Oicha | Recurrence | Yes | 3 | 0.0 |
| Rimba | Recurrence | Yes | 2 | 0.0 |
| Vuhovi | Recurrence | Yes | 4 | 0.0 |
| Gety | Censored | Yes | 3 | 0.0 |
| Goma | Censored | No | 0 | 70.8 |
| Kambala | Censored | Yes | 2 | 0.0 |
| Lubunga | Censored | Yes | 1 | 0.0 |
| Mabalako | Censored | Yes | 2 | 0.0 |
| Mangobo | Censored | Yes | 1 | 0.0 |
| Miti-Murhesa | Censored | No | 0 | 236.0 |
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