Submitted:
11 March 2026
Posted:
13 March 2026
You are already at the latest version
Abstract
Keywords:
1. Introduction
2. Materials and Methods
2.1. Study Design and Setting
2.2. Study Questionnaire Design
- Demographic information—sex, age, country of birth, university and year of study, main academic discipline.
- Knowledge of Mpox—9 items evaluating knowledge about the virus, its transmission, clinical characteristics, and prevention. The questions were adapted from Harapan et al. [14]. Correct responses received a score of 2, incorrect responses were scored as 0, and “I do not know” was given a score of 1.
- Attitudes toward conspiracy beliefs regarding emerging diseases—eight items assessing respondents’ perceptions of global and local responses to outbreaks. A 5-point Likert scale was used (“Strongly agree” = 5 to “Strongly disagree” = 1), with a maximum score of 40. We adopted survey items from a study by Freeman at al. [15] on SARS-CoV-2 conspiracy beliefs. Higher scores indicated a greater embrace of conspiracy beliefs regarding virus emergence and subsequent intervention measures.
2.3. Statistical Analysis
3. Results
3.1. Characteristics of the Respondents
| Variable | All Respondents (n = 1313) n, (%) |
|---|---|
| Age, mean ± Std | 22.63 ± 4.440 |
|
Gender, n (%) Male Female |
473 (36.0) 840 (64.0) |
|
University Medical University of Sofia Medical University of Plovdiv Medical University of Skopje Medical University of Stip |
255 (19.4) 793 (60.4) 232 (17.7) 33 (2.5) |
|
Major Medicine Dental Medicine Pharmacy Other |
933 (71.1) 161 (12.3) 145 (11.0) 74 (5.6) |
|
Year of study 1st year 2nd year 3rd year 4th year 5th year 6th year |
156 (11.9) 154 (11.7) 305 (23.2) 205 (15.6) 394 (30.0) 99 (7.5) |
3.2. Knowledge About Human Mpox

| Human Mpox Knowledge Item |
Response | Age | p-Value | Sex | p-Value | ||
|---|---|---|---|---|---|---|---|
| <21 years (n = 528) |
>21years (n = 785) |
Male (n = 473) |
Female (n = 840) |
||||
| Mpox is caused by a virus. | Correct Incorrect Do not know |
322 (61.0) 33 (6.2) 173 (32.8) |
644 (82.0) 32 (6.7) 89 (11.3) |
<0.001 | 359 (75.9) 26 (5.5) 88 (18.6) |
627 (74.6) 39 (4.7) 174 (20.7) |
0.853 |
| Mpox is easily transmitted among humans. | Correct Incorrect Do not know |
78 (14.8) 232 (44.0) 218 (41.2) |
209 (26.6) 369 (47.0) 206 (26.4) |
<0.001 | 128 (27.1) 194 (41.0) 151 (31.9) |
159 (18.9) 407 (48.4) 274 (32.7) |
0.002 |
| Mpox is transmitted through the bite and saliva of an infected monkey. | Correct Incorrect Do not know |
126 (23.9) 217 (41.1) 185 (35.0) |
239 (30.4) 354 (45.1) 189 (24.5) |
<0.001 |
139 (29.4) 193 (40.8) 141 (29.8) |
226 (26.9) 378 (45.0) 236 (28.1) |
0.419 |
| Skin rash is one of the signs or symptoms of human Mpox. | Correct Incorrect Do not know |
330 (62.5) 18 (3.4) 180 (34.1) |
572 (72.9) 31 (3.9) 182 (23.2) |
<0.001 |
300 (63.4) 24 (5.1) 149 (31.5) |
602 (71.7) 25 (3.0) 213 (25.3) |
0.005 |
| Mpox and chickenpox have similar signs and symptoms. | Correct Incorrect Do not know |
201 (38.1) 44 (8.3) 283 (53.6) |
377 (48.0) 105 (13.4) 303 (38.6) |
<0.001 |
185 (39.1) 65 (11.6) 233 (49.3) |
393 (46.8) 84 (10.0) 363 (43.2) |
0.012 |
| Pustule is one of the signs or symptoms of human Mpox. | Correct Incorrect Do not know |
258 (48.9) 24 (4.5) 246 (46.6) |
455 (58.0) 49 (6.2) 281 (35.8) |
<0.001 |
234 (49.5) 32 (6.7) 207 (43.8) |
479 (57.0) 41 (4.9) 320 (38.1) |
0.024 |
| Diarrhea is one of the signs and symptoms of human Mpox. | Correct Incorrect Do not know |
58 (11.0) 80 (15.1) 390 (73.9) |
164 (20.9) 143 (18.2) 478 (60.9) |
<0.001 | 90 (19.0) 78 (16.5) 305 (64.5) |
132 (15.7) 145 (17.3) 563 (67.0) |
0.303 |
| Antibiotics are used to treat human Mpox. | Correct Incorrect Do not know |
130 (24.6) 95 (18.0) 303 (57.4) |
371 (47.3) 131 (16.7) 283 (36.0) |
<0.001 | 196 (41.4) 67 (13.8) 212 (44.8) |
305 (36.3) 159 (18.9) 376 (44.8) |
0.048 |
| Vaccination is available to prevent human Mpox. | Correct Incorrect Do not know |
85 (16.1) 128 (24.3) 315 (59.6) |
232 (29.5) 204 (26.0) 349 (44.5) |
<0.001 | 110 (23.2) 113 (23.9) 250 (52.9) |
207 (24.6) 219 (26.1) 414 (49.3) |
0.549 |
| Conspiracy Belief Statements | Completely Disagree (n, %) |
Disagree (n, %) | Neither Agree Nor Disagree (n, %) | Agree (n, %) | Completely Agree (n, %) |
|---|---|---|---|---|---|
| Mpox spread worldwide due to the role of men having sex with men. | 138 (10.5) |
203 (15.5) |
523 (39.8) |
310 (23.6) | 139 (10.6) |
| I am skeptical of the official explanation for the causes of Mpox. | 76 (5.8) |
236 (18.0) |
692 (52.7) |
247 (18.8) | 62 (4.7) |
| I do not trust the information I receive from experts on emerging infectious diseases. | 232 (17.7) |
616 (46.9) |
316 (24.1) |
109 (8.3) |
40 (3.0) |
| Many bacteria, viruses, parasites, and fungi have been created in laboratories. | 104 (7.9) |
295 (22.5) |
505 (38.5) |
325 (24.8) | 84 (6.4) |
| The spread of infectious pathogens (bacteria, viruses, parasites, and mycoses) is a deliberate attempt by some organizations to reduce the human population. | 174 (13.3) |
335 (25.5) |
465 (35.4) |
252 (19.2) | 87 (6.6) |
| The lockdown as a response to emerging infections aims to ensure destabilization and control over the economy and financial system of a large part of the countries around the world. | 169 (12.9) |
325 (24.8) |
426 (32.4) |
278 (21.2) | 115 (8.8) |
| The lockdown is a way and means to change society to satisfy the financial and economic interests of certain “shadow” organizations. | 166 (12.6) |
361 (27.5) |
455 (34.7) |
239 (18.2) | 92 (7.0) |
| Very often, the media purposefully provides incorrect information about certain infections and measures to contain them. | 89 (6.8) |
241 (18.4) |
415 (31.6) |
398 (30.3) | 170 (12.9) |
4. Discussion
5. Limitations
6. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Riccò, M.; Ferraro, P.; Camisa, V.; Satta, E.; Zaniboni, A.; Ranzieri, S.; Baldassarre, A.; Zaffina, S.; Marchesi, F. When a Neglected Tropical Disease Goes Global: Knowledge, Attitudes and Practices of Italian Physicians towards Monkeypox, Preliminary Results. Trop. Med. Infect. Dis. 2022, 7, 135. [Google Scholar] [CrossRef]
- Mitjà, O.; Ogoina, D.; Titanji, B.K.; Galvan, C.; Muyembe, J.J.; Marks, M.; Orkin, C.M. Monkeypox. Lancet 2023, 401, 60–74. [Google Scholar] [CrossRef]
- Bunge, E.M.; Hoet, B.; Chen, L.; Lienert, F.; Weidenthaler, H.; Baer, L.R.; Steffen, R. The Changing Epidemiology of Human Monkeypox—A Potential Threat? A Systematic Review. PLoS Negl. Trop. Dis. 2022, 16, e0010141. [Google Scholar] [CrossRef]
- World Health Organization. WHO Director-General Declares Mpox Outbreak a Public Health Emergency of International Concern. 2024. Available online: https://www.who.int/news/item/14-08-2024-who-director-general-declares-mpox-outbreak-a-public-health-emergency-of-international-concern (accessed on 30 January 2026).
- Global Mpox Trends. 2026. Available online: https://worldhealthorg.shinyapps.io/mpx_global/#sec-global (accessed on 20 February 2026).
- Titanji, B.K.; Hazra, A.; Zucker, J. Mpox Clinical Presentation, Diagnostic Approaches, and Treatment Strategies: A Review. JAMA 2024, 332, 1652–1662. [Google Scholar] [CrossRef]
- Riad, A.; Attia, S. Monkeypox-Related Oral Manifestations and Implications: Should Dentists Keep an Eye Out? J. Med. Virol. 2023, 95, e28091. [Google Scholar] [CrossRef]
- Nuzzo, J.B.; Borio, L.L.; Gostin, L.O. The WHO Declaration of Monkeypox as a Global Public Health Emergency. JAMA 2022, 328, 615–616. [Google Scholar] [CrossRef] [PubMed]
- Alshahrani, N.Z.; Mitra, S.; Alkuwaiti, A.A.; Alhumam, M.N.; Altmimi, S.M.B.; Alamri, M.H.M.; Albalawi, Z.A.S.; Almorgi, M.W.; Alharbi, H.K.D.; Alshahrani, S.M. Medical Students’ Perception Regarding the Re-Emerging Monkeypox Virus: An Institution-Based Cross-Sectional Study From Saudi Arabia. Cureus 2022, 14, e28060. [Google Scholar] [CrossRef] [PubMed]
- Sallam, M.; Al-Mahzoum, K.; Dardas, L.A.; Al-Tammemi, A.B.; Al-Majali, L.; Al-Naimat, H.; Jardaneh, L.; AlHadidi, F.; Al-Salahat, K.; Al-Ajlouni, E.; et al. Knowledge of Human Monkeypox and Its Relation to Conspiracy Beliefs among Students in Jordanian Health Schools: Filling the Knowledge Gap on Emerging Zoonotic Viruses. Medicina 2022, 58, 924. [Google Scholar] [CrossRef]
- European Centre for Disease Control and Prevention (ECDC). Communicable Diseases Threats Report, 17 February 2023. Available online: https://www.ecdc.europa.eu/sites/default/files/documents/communicable-disease-threats-report-17-feb-2023_0.pdf (accessed on 30 January 2026).
- Health Ministry: Two Monkeypox Cases Confirmed in Bulgaria, 23 June 2022. Available online: https://sofiaglobe.com/2022/06/23/health-ministry-two-monkeypox-cases-confirmed-in-bulgaria/ (accessed on 30 January 2026).
- Polit, D.F.; Beck, C.T.; Owen, S.V. Is the CVI an acceptable indicator of content validity? Appraisal and recommendations. Res. Nurs. Health 2007, 30, 459–467. [Google Scholar] [CrossRef] [PubMed]
- Harapan, H.; Setiawan, A.M.; Yufika, A.; et al. Knowledge of human monkeypox viral infection among general practitioners: A cross-sectional study in Indonesia. Pathog. Glob. Health 2020, 114, 68–75. [Google Scholar] [CrossRef]
- Freeman, D.; Waite, F.; Rosebrock, L.; Petit, A.; Causier, C.; East, A.; Jenner, L.; Teale, A.-L.; Carr, L.; Mulhall, S.; et al. Coronavirus conspiracy beliefs, mistrust, and compliance with government guidelines in England. Psychol. Med. 2020, 52, 251–263. [Google Scholar] [CrossRef]
- Doan, D.A.; Nguyen, T.T.X.; Le, G.B.; Phuong, T.L.; Nguyen, P.L.; Dinh, D.X. Knowledge and attitudes of medical and pharmacy university students regarding monkeypox: A multicenter, cross-sectional study in Vietnam. BMC Med. Educ. 2024, 24, 807. [Google Scholar] [CrossRef]
- Yu, L.; Shen, Y.; Liu, M.; Ma, J.; Long, J.; Zheng, D. Evaluation of monkeypox knowledge and attitudes among Chinese medical students. BMC Public Health 2024, 24, 745. [Google Scholar] [CrossRef]
- Mullan, J.; Burns, P.; Weston, K.; McLennan, P.; Rich, W.; Crowther, S.; Mansfield, K.; Dixon, R.; Moselen, E.; Osborne, R.H. Health literacy amongst health professional university students: A study using the Health Literacy Questionnaire. Educ. Sci. 2017, 7, 54. [Google Scholar] [CrossRef]
- Castro-Sánchez, E.; Chang, P.W.S.; Vila-Candel, R.; Escobedo, A.A.; Holmes, A.H. Health literacy and infectious diseases: Why does it matter? Int. J. Infect. Dis. 2016, 43, 103–110. [Google Scholar] [CrossRef] [PubMed]
- Bunge, E.M.; Hoet, B.; Chen, L.; Lienert, F.; Weidenthaler, H.; Baer, L.R.; Steffen, R. The changing epidemiology of human monkeypox—A potential threat? A systematic review. PLoS Negl. Trop. Dis. 2022, 16, e0010141. [Google Scholar] [CrossRef]
- van Mulukom, V.; Pummerer, L.J.; Alper, S.; et al. Antecedents and consequences of COVID-19 conspiracy beliefs: A systematic review. Soc. Sci. Med. 2022, 301, 114912. [Google Scholar] [CrossRef]
- Ripp, T.; Röer, J.P. Systematic review on the association of COVID-19-related conspiracy belief with infection-preventive behavior and vaccination willingness. BMC Psychol. 2022, 10, 66. [Google Scholar] [CrossRef]
- Almulhem, J.; Aldekhyyel, R. Medical Students’ Ability to Assess Health Information Accuracy. Stud. Health Technol. Inform. 2025, 329, 1397–1401. [Google Scholar] [CrossRef]
- Enders, A.; Klofstad, C.; Diekman, A.; Drochon, H.; Rogers de Waal, J.; Littrell, S.; Premaratne, K.; Verdear, D.; Wuchty, S.; Uscinski, J. The sociodemographic correlates of conspiracism. Sci. Rep. 2024, 14, 14184. [Google Scholar] [CrossRef]
- Baralin, A.; Boulares, E.; Tan, S.C.Y.; Shin, Y.J. Identifying Strengths and Weaknesses in Health Literacy among Medical Students: A Systematic Review. BMC Med. Educ. 2025, 25, 1424. [Google Scholar] [CrossRef]
- Araújo, B.; Gomes, S.F.; Ribeiro, L. Critical thinking pedagogical practices in medical education: A systematic review. Front. Med. 2024, 11, 1358444. [Google Scholar] [CrossRef] [PubMed]
- Arora, V.M.; Krishnamoorthi, V.R.; Bloomgarden, E.; Jain, S.; Sunderrajan, A.; Tesema, N.; Zietowski, M.; Farnan, J.; Serritella, S. Restoring Trust in Science and Medicine: Empowering and Educating Future Physicians in Science Communication With the Public. Acad. Med. 2023, 98, S30–S33. [Google Scholar] [CrossRef]
- Krohn, K.M.; Crichlow, R.; McKinney, Z.J.; Tessier, K.M.; Scheurer, J.M.; Olson, A.P.J. Introducing Mass Communications Strategies to Medical Students: A Novel Short Session for Fourth-Year Students. Acad. Med. 2022, 97, 999–1003. [Google Scholar] [CrossRef]
- World Health Organization. Risk Communication and Community Engagement Readiness and Response Toolkit: Mpox. 2024. Available online: https://www.who.int/publications/i/item/9789240091559 (accessed on 30 January 2026).
- Thornhill, J.P.; Antinori, A.; Orkin, C.M. Monkeypox Virus Infection across 16 Countries—April–June 2022. Reply. N. Engl. J. Med. 2022, 387, e69. [Google Scholar] [CrossRef]
- Paterson, A.; Cheyne, A.; Tulunay, H.; Orkin, C.; Nutland, W.; Dunning, J.; Stolow, J.; Gobat, N.; Olliaro, P.; Rojek, A. Mpox stigma in the UK and implications for future outbreak control: A cross-sectional mixed methods study. BMC Med. 2025, 23, 422. [Google Scholar] [CrossRef] [PubMed]
- Linares-Navarro, R.; Sanz-Muñoz, I.; Onecha-Vallejo, V.; Fernandez-Espinilla, V.; Eiros, J.M.; Castrodeza-Sanz, J.; Prada-Garcia, C. Psychosocial impact and stigma on men who have sex with men due to monkeypox. Front. Public Health 2025, 13, 1479680. [Google Scholar] [CrossRef]
- Charlier, C.; Johannessen, I.; Mackintosh, C.L.; Wilks, D.; Cauda, R.; Wolf, F.I.; Jeunne, C.L. International infectious diseases teaching to undergraduate medical students: A successful European collaborative experience. Med. Teach. 2017, 39, 981–986. [Google Scholar] [CrossRef]
- Bendezu-Quispe, G.; Torres-Roman, J.S.; Salinas-Ochoa, B.; Hernández-Vásquez, A. Utility of massive open online courses (MOOCs) concerning outbreaks of emerging and reemerging diseases. F1000Reserch 2017, 6, 1699. [Google Scholar] [CrossRef]
- World Medical Association. World Medical Association Declaration of Helsinki: Ethical principles for medical research involving human subjects. JAMA 2013, 310, 2191–2194. [Google Scholar] [CrossRef]

Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2026 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).