Various Mixtures of Carbon Negative Replacements of Gasoline and Diesel Fuel

The Author has received all the proper documents granting the Ethical Approval and the Consent to Participate from the State of Texas officials. The Author has made sure that the ethical approval and his consent to participate in preparation and submission for publication of this article were properly approved by the respective authorities of the State of Texas.

The Author has expressed his complete consent to participate in work with this article and its publication in this Journal.

The Author makes all his data and materials herein available for any third party. The data and materials might be obtained from the Author at PO Box 300230, Houston, TX, 77340. Email drmtyurin76@outlook.com. If any third party needs any materials used to publish this article, please, do contact the Author.

This article is another the Author’s move in the war which has started by SHELL International Petroleum Corporation some time back by attempted murder of the Author. The people hired by SHELL has totaled the Author’s corporate car and did some other illegal things to the Author which have concluded the Author that the war with the international petroleum corporations has started already. No Houston FBI or Houston Police investigation of said attempted murder has happened and this is behind the responsibility of the Texas Governor with all the legal remedies the Author might use in his fight to sue the State and its Governor Gregory Abbott. The attempted murder has the statute of limitations 20 years, and there are jurisdictions above the level of the State of TEXAS. The Author had certain legal problems with the at that time Attorney General of the State of Texas in 2013, now Texas Governor, Greg Abbott and the Author is going to resolve all the legal problems with the Texas Governor and the State of TX (TX Police, FBI, etc.), regardless would he be dismissed from his postion or not. The corporate Author’s website http://syngasbiofuelsenergy.com was destroyed by the person, the Attorney of Hirsch and Westheimer Law Firm, PC by the last name Levy on December 20, 2020. She belongs to the same family which owned or owns the grocery stores chain named “Fiesta” in Houston Texas. The Author was unable to find a lawyer in Texas to file the respective federal law suit and recover his corporate website, but the Author has the US Constitutional right to recite this website in the references herein. Therefore the Author has multiple legal problems with the State of Texas and the Author is going to resolve said legal problems at the respective Court level in the US to punish the Stae and its Givernor. The market vaue of the Author currently is over $1.5 trillion and it is growing daily https://www.mordorintelligence.com/industry-reports/gasoline-as-a-fuel-market/market-size). The Author is already involved into the war which has been started by the International Petroleum Corporation SHELL. The Author has approached SHELL offering them for the commercialization the proprietary technology of Gasoline or Diesel fuel manufacture from the air CO₂, not from petroleum which SHELL and other international petroleum corporations use. SHELL representative met the Author, got his draft of the at that time in publication article on creation Gasoline from the air CO₂ [1,21–24,41,42]. Then SHELL hired two Mexicans to kill Dr. The Author in the car accident, paying the possibly around $6,000 for this dirty job, which has been done in Houston TEXAS at the US59 down South (9494 Southwest Fwy). The Houston FBI and the Houston Police were contacted by the Author multiple times over his mobile phone however there was no any detailed and thorough Police or the FBI investigation of said attempted murder of the Author since as the Author trusts, both Houston FBI and Houston Police are totally corrupted by the international petroleum corporations. Therefore any law is not used in the State of Texas to hurt said corporations. Therefore the Author has to look for the law enforcement outside of the State of Texas, at the National level and he will do that to affect the Texas Governor Abbott for his actions of 2013 and have him resign from his position. Therefore the Author has conflict of interests with the State of Texas, TEXAS Governor Gregg Abbott, Texas FBI and Texas Police. Since that time more scientific publications of the Author on the Gasoline and Diesel fuel production from the air CO₂ came out [15–49]. The US patents are extremely expensive, therefore the Author uses instead of patents scientific publications. Publications are as good as the US patents, each the US patent has to claim something better than the Author already did in his scientific publications. The Author working on the Acetogens-biocatalysts has no any competition in the world because of his prior invention, the electroporation / electrofusion Generator, already sold as a sample (with no right for reproduction) to the US corporation BTX, Inc. / Genetronics, Inc. (San-Diego, CA). Said Generator and the invented by the Author genome tailoring technology make him proud to be with no any competition in the whole World. Mentioning of names Dr. Gak, Dr. Kiriukhin, Dr. Padda in certain publications herein and elsewhere (Tyurin MV, Padda RS. (2019) Nitrogen gas reducing commercial acetogen biocatalyst suitable for direct and selective reduction of CO2 inorganic carbon to organic carbon and atmospheric nitrogen to fuel isobutanol during continuous fermentation of CO₂ + H₂ + N₂ gas blend. International Research Journal of Applied Sciences, Engineering and Technology. V.3, 4, 1 - 10; Tyurin MV, Padda R, Huang K-X, Wardwell S, Caprette D, Bennett GN (2000) Electrotransformation of Clostridium acetobutylicum ATCC 824 using high-voltage radio frequency modulated square pulses // J Appl Microbiol. 88(2):220-227; Gak E, Tyurin MV, Kiriukhin M (2014) Genome tailoring powered production of isobutanol in continuous CO₂ / H₂ blend fermentation using engineered acetogen biocatalyst. J Ind Microbiol Biotechnol. 41(5):763-781 http://www.ncbi.nlm.nih.gov/pubmed/24659176; Kiriukhin M, Tyurin MV, Gak E (2014) UV-induced mutagenesis in acetogens: resistance to methanol, ethanol, acetone, or n-butanol in recombinants with reduced genomes during continuous CO₂ / H₂ gas blend fermentation. World Journal of Microbiology and Biotechnology. 30(5):1559-1574. https://link.springer.com/article/10.1007/s11274-013-1579-7; Berzin V, Kiriukhin M., Tyurin M. (2012) Elimination of acetate production to improve ethanol yield during continuous synthesis gas fermentation by engineered biocatalyst Clostridium sp. MTEtOH550. Appl Biochem Biotechnol. 167 (2):338-347. https://link.springer.com/article/10.1007/s12010-012-9697-5; Berzin V, Tyurin M. (2012). Acetogen biocatalyst Clostridium sp. MTEtOH871 engineered with our proprietary electrotransformation technology and equipment: continuous synthesis gas fermentation for selective ethanol production. Journal of Biotech Research. 4:54-64; Berzin V, Kiriukhin M, Tyurin M. (2013) “Curing” of plasmid DNA in acetogen using microwave or applying an electric pulse improves cell growth and metabolite production as compared to the plasmid-harboring strain. Arch. Microbiol. 195(3), 181-188; Berzin V, Kiriukhin M, Tyurin M. (2013) Selective n-butanol production by Clostridium sp. MTButOH1365 during continuous synthesis gas fermentation due to expression of synthetic thiolase, 3-hydroxy butyryl-CoA dehydrogenase, crotonase, butyryl-CoA dehydrogenase, butyraldehyde dehydrogenase and NAD-dependent butanol dehydrogenase. Appl Biochem Biotechnol. 169(3), 950-959. https://link.springer.com/article/10.1007/s12010-012-0060-7; Berzin V, Kiriukhin M, Tyurin M (2013) Cre-lox66/lox71-based elimination of phosphotransacetylase or acetaldehyde dehydrogenase shifted carbon flux in acetogen rendering selective overproduction of ethanol or acetate. Appl Biochem Biotechnol. 195(3):181-8. https://link.springer.com/article/10.1007/s12010-012-9864-8; Tyurin M, Kiryukhin M, Berzin V. (2012) Electrofusion of untreated cells of the newly isolated acetogen Clostridium sp. MT351 with integrated in the chromosome erm(B) or cat leading to the combined presence of these antibiotic resistance genes in the chromosome of the electrofusion products. Journal of Biotech Research. 4:1-12; Tyurin M, Kiriukhin M. (2013). Expression of amplified synthetic ethanol pathway integrated using Tn7-tool and powered at the expense of eliminated pta, ack, spo0A and spo0J during continuous syngas or CO₂ /H₂ blend fermentation. J Appl Microbiol. 114(4):1033-45. https://ami-journals.onlinelibrary.wiley.com/doi/abs/10.1111/jam.12123; Berzin V, Kiriukhin M, Tyurin MV (2012) Selective production of Acetone during continuous synthesis gas fermentation by engineered biocatalyst Clostridium sp. MAceT113. Letters of Appl Microbiol. 55(2):149-54. . https://doi.org/10.1111/j.1472-765X.2012.03272.x. https://ami-journals.onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2012.03272.x; Tyurin MV, Kiriukhin M. (2013). Selective methanol or formate production during continuous CO₂ fermentation by the acetogen biocatalysts engineered via integration of synthetic pathways using Tn7-tool. World Journal of Microbiology and Biotechnology. 29 (9)1611-1623. https://www.semanticscholar.org/paper/Selective-methanol-or-formate-production-during-CO2-Tyurin-Kiriukhin/84f55f6505b1757dcab27f93a532001f035eb53b; Tyurin MV, Kiriukhin M (2013). 2,3-Butanediol production by engineered acetogen biocatalyst during continuous fermentation of syngas or CO₂/H₂ blend. Appl Biochem Biotechnol. 170 (6): 1503-1524. doi: 10.1007/s12010-013-0285-0; Kiriukhin M, Tyurin MV, Gak E (2014) UV-induced mutagenesis in acetogens: resistance to methanol, ethanol, acetone, or n-butanol in recombinants with reduced genomes during continuous CO₂ / H₂ gas blend fermentation. World Journal of Microbiology and Biotechnology. 30(5):1559-1574. https://link.springer.com/article/10.1007/s11274-013-1579-7; Kiriukhin M, Tyurin MV (2013) Mevalonate production by engineered acetogen biocatalyst during continuous fermentation of syngas or CO₂/H₂ blend. Bioprocess Biosyst Eng. 2014 Feb;37(2):245-260. doi: https://pubmed.ncbi.nlm.nih.gov/24415498/; Kiriukhin M, Tyurin MV (2013) Mevalonate production by engineered acetogen biocatalyst during continuous fermentation of syngas or CO₂/H₂ blend. Bioprocess Biosyst Eng. 2014 Feb;37(2):245-260. doi: https://pubmed.ncbi.nlm.nih.gov/24415498/; Gak E, Tyurin MV, Kiriukhin M (2014) Genome tailoring powered production of isobutanol in continuous CO₂ / H₂ blend fermentation using engineered acetogen biocatalyst. J Ind Microbiol Biotechnol. 41(5):763-781 http://www.ncbi.nlm.nih.gov/pubmed/24659176) has no practical authorship of said publications meaning since said individuals got paid by the Author significant amounts of cash for doing research for the Author per the topics he ordered them to investigate and provide the detailed reports to him. However their names are included in said specific publications since they have worked on said topics. Name of Vel G. Berzin is also included and has no scientific meaning since Mr. Berzin was a friend of the Author some time back, Mr. Berzin paid his own $300 for starting the corporation Syngas Biofuels Energy, Inc. for which he did not provide any scientific input including the corporate website http://syngasbiofuelsenergy.com. Mr. Berzin has retired from the public life when he became 65 and he keeps his current location at the nursing home very confidential for the Author.

The Author has received no funding. The investors of the Author has read this article draft to his investors and they have decided not to include no funding information as they have stated to the Author. They stated the Author should write “No Funding” per this original article. No funding. No funding was used to prepare and publish this original article. The available investors have declined to provide their information for this publication. They have suggested to note that the article was prepared and published with no any funding source.

The Author has conducted all the experiments himself. The Author has planned, wrote this original article and edited the written text, including proper placement of the illustrations mentioned which the Author owns. The Author read, edited and approved the final manuscript. The Author is the only owner of all materials disclosed in this original article. The Author has plans to distribute his proprietary products after their approval as needed. The Author might be contacted for the data and materials at PO Box 300230, Houston, TX, 77230, drmtyurin76@outlook.com. The Author contributed to the study conception and design. Material preparation, data collection and analysis were performed by the Author. The first draft of the manuscript was written by the Author. The Author read and approved the final manuscript. The Author has designed the ideology of this article by himself. The Author intends to develop the detailed structure and location of his Carbon Negative corporations to be in charge for the establishing and running testing trials of his inventions he plans to commercialize additionally to his major business of manufacture of Carbon Negative fuels and Carbon Negative genetically engineered foods for the Nationwide distribution. The Author has conducted all the experiments himself. The Author has planned, wrote this original article and edited the written text, including proper placement of the illustrations mentioned which the Author owns. The Author read, edited and approved the final manuscript. The Author is the only owner of all materials disclosed in this original article. The Author has plans to distribute his proprietary products after their approval as needed. The Author might be contacted for the data and materials at PO Box 300230, Houston, TX, 77230, drmtyurin76@outlook.com. The Author contributed to the study conception and design. Material preparation, data collection and analysis were performed by the Author. The first draft of the manuscript was written by the Author. The Author read and approved the final manuscript. The Author has designed the ideology of this article by himself. The Author intends to develop the special structure at his Carbon Negative corporations to be in charge for the establishing and running clinical trials of his inventions he plans to commercialize additionally to his major business of manufacture of Carbon Negative fuels and Carbon Negative genetically engineered foods for the Nationwide distribution. The Author conceived of the presented idea. The Author developed the theory and performed the computations. The Author verified the analytical methods. The Author investigated therapeutic effects of “Mixture” and supervised the findings of this work. The Author discussed the results and contributed to the final manuscript. The Author carried out the experiment. The Author wrote the manuscript. The Author supervised the project. The Author conceived the original idea. The Author supervised the project. The Author developed the theoretical formalism, performed the analytic calculations and performed the numerical simulations. The Author contributed to the final version of the manuscript. The Author supervised the project. The Author conceived and planned the experiments. The Author carried out the experiments. The Author planned and carried out the simulations. The Author contributed to sample preparation. The Author contributed to the interpretation of the results. The Author took the lead in writing the manuscript. The Author provided critical feedback and helped shape the research, analysis and manuscript. The Author designed the model and the computational framework and analyzed the data. The Author carried out the implementation. The Author performed the calculations. The Author wrote the manuscript. The Author conceived the study and were in charge of overall direction and planning. The Author designed and performed the experiments, derived the models and analyzed the data. The Author wrote the manuscript in consultation with other corporate employees, devised the project, the main conceptual ideas and proof outline. The Author worked out almost all of the technical details, and performed the numerical calculations for the suggested experiment. The Author worked out the bound for quantum mechanics. The Author analyzed the data. The Author wrote the paper. The Author designed and directed the project; the Author performed the experiments; the Author analyzed spectra; the Author. made the simulations; the Author developed the theoretical framework; the Author wrote the article. The Author performed the measurements. The Author. was involved in planning and supervised the work, the Author processed the experimental data, performed the analysis, drafted the manuscript and designed and made the figures.

The Author has received no funding. The investors of the Author has read this article draft to his investors and they have decided not to include no funding information as they have stated to the Author. They stated the Author should write “No Funding” per this original article. No funding. No funding was used to prepare and publish this original article. The available investors have declined to provide their information for this publication. They have suggested to note that the article was prepared and published with no any funding source.

The Author has wrote this original article based on his originality of the business approach and the existing resistance of the International Petroleum corporation to the technology of manufacturing carbon negative fuels to replace their production of fuels originating from petroleum. The reduction of the air CO₂ levels towards the pre-petroleum era of the year 1900 is paramount,since our planet looses fresh water to the outer Space vacuum. NASA has confirmed that in 2010 sating that the Earth has reached the “Point of No Return” to the healthy environmental conditions suitable for life on our planet. The new family of the gas stations selling not only Carbon Negative fuels but also foods for cooking at home and commodity chemicals for the households will save a lot of time for the customers of said new gas stations. The Author Dr. Michael V. Tyurin is the Author of this original article. In 2017 after tragic death of his wife Prof. Dr. Tyurina Aleksandra Nicolaevna his father Prof. Dr. Tyurin Vladimir Inljich decided to change his first name from “Vladimir” to “Vladislav”. Therefore the Author Dr. MV Tyurin had to change his second name from “Vladimirovich” to “Vladislavovich”. ORCID # is 0000-0001-6943-6556. Contact address: Dr. Michael Vladislavovich Tyurin, CEO, Executive Chairman, President Microbial Biocatalyst International, Inc. and Inorgcarbdiesel, Inc. PO Box 300230, Houston, TX 77230. Contact Email drmtyurin76@outlook.com.