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The Potential Use of Arsenic Trioxide in the Treatment of Systemic Lupus Erythematosus

A peer-reviewed version of this preprint was published in:
International Journal of Molecular Sciences 2024, 25(17), 9577. https://doi.org/10.3390/ijms25179577

Submitted:

11 August 2024

Posted:

13 August 2024

You are already at the latest version

Abstract
Arsenic trioxide (ATO) is now part of the standard regimen for the treatment of newly diagnosed and relapsed acute promyelocytic leukaemia. The availability of an oral form of ATO has greatly reduced the incidence of cardiotoxicity as compared to intravenous administration. Increasing evidence suggests that ATO has anti-inflammatory properties that may be useful for the treatment of autoimmune diseases. These include the modulation of Treg cell activation, Th1/Th2 and Th17/Treg balance, depletion of activated T cells and plasmacytoid dendritic cells, and influence of B cell differentiation, leading to reduced autoantibody and cytokine production. ATO has also been shown to induce apoptosis of activated fibroblast-like synoviocytes through generation of reactive oxygen species and alter the gut microbiota in collagen-induced arthritis. Despite the emergence of newer treatment modalities, the treatment of systemic lupus erythematosus (SLE), especially refractory manifestations, remains a challenge, owing to the paucity of effective biological and targeted therapies that are devoid of adverse effects. Oral ATO is an attractive option for the treatment of SLE because of the lower cost of production, convenience of administration and reduced cardiotoxicity. This article summarizes the anti-inflammatory mechanisms of ATO and its potential application in the treatment of SLE.
Keywords: 
arsenic trioxide
;  
non-leukemic
;  
autoimmune
;  
immune-mediated
;  
rheumatic
;  
lupus
Subject: 
Public Health and Healthcare  -   Public Health and Health Services

1. Introduction

Arsenic is an element commonly found in nature and exists with organic and inorganic forms. While arsenic and its compounds are well known to be a means of poisoning, their medicinal use dates back to ancient Greece and Rome when arsenic was viewed as both a therapeutic agent and a poison [1]. The arsenicals were probably introduced into western medicine at around the eighteenth century [2] for the treatment of chronic leukemia. The utility of arsenic in the treatment of hematological malignancies is limited by its toxicities, in particular cardiological. Interest in the therapeutic use of intravenous (IV) arsenic trioxide (ATO) has recently been rekindled due to promising reports from mainland China highlighting its efficacy and cost-effectiveness in acute promyelocytic leukemia (APL) [3].
The introduction of a combination regimen of all-trans retinoic acid (ATRA) and ATO in the 1990s has turned APL, a highly fatal condition, to a highly curable condition. ATO as a single agent can induce remission of ≥70% of APL patients whereas ATO combined with ATRA can induce remission of at least 90% of APL patients [4]. Trisenox, an injectable form of ATO, used in conjunction with ATRA, was approved by the United States (US) Food and Drug administration (FDA) in 2018 for the treatment of adults with newly diagnosed low-risk APL characterized by the presence of the t(15;17) translocation or promyelocytic leukemia/retinoic acid receptor alpha (PML/RARα) gene expression [5]. A phase III clinical trial from mainland China demonstrated that the overall survival rate for APL patients who received a combination of Trisenox and ATRA reached 96.6% at 3 years, with disease relapse occurring in less than 1% of the patients [6].
Although ATO is now part of the standard regimen for the treatment of newly diagnosed and relapsed APL [7,8], concerns about the IV preparation of ATO include inconvenience, need for hospitalization, vascular access, cost, as well as dose-related cardiotoxicity such as ventricular arrythmias and sudden cardiac death [9]. An oral solution of ATO is currently available in Hong Kong, China, with a bioavailability similar to that of the IV preparation [10,11]. The cardiac safety of the oral preparation is much more favorable than the IV preparation. Adverse effects of oral ATO in the treatment of acute leukemia are usually minor and respond to symptomatic treatment or temporary drug cessation [12]. These include skin reactions, gastrointestinal upset and hepatitis. In a long-term observational study of oral ATO (10mg/day) for the treatment of relapsed APL, the commonest reported non-hematological AEs were elevation of liver parenchymal enzymes, headache, infections, skin rash and gastrointestinal upset (nausea, vomiting) [13]. Three patients developed asymptomatic QTc prolongation. A more detailed study on cardiac rhythm in APL patients treated with oral ATO (10mg/day) showed that transient prolongation of QTc to more than 500ms occurred in 18% of patients 4 hours after ingestion [14]. Premature ventricular beats were no more common in ATO users and none of the patients developed ventricular tachyarrhythmia.

2. Pharmacokinetics of Arsenic Trioxide

After IV administration, ATO is distributed throughout the body before it is metabolized in the liver. The drug undergoes hepatic methylation to become water-soluble methylated (MAs) and dimethylated (DMAs) metabolites, which are generally less toxic and excreted predominantly by the kidneys [15]. In a Japanese study [16], the plasma concentrations of inorganic arsenics (iAs) reached Cmax rapidly after IV administration of ATO on the first day. During repeated administration, the plasma concentrations of iAs reached a steady state but the mean total arsenic excretion rate (including iAs and methylated arsenic) was about 20% of the daily dose on the first day and remained at about 60% of the daily dose during the first to fourth week. Recent studies also demonstrated that several factors might affect the effective plasma concentration of ATO after IV administration [17,18,19]. These included polymorphisms of the gene encoding AS3MT that is important for methylation of the iAs [17,18], smoking and prior chemotherapy exposure [19].
Arsenol is an oral ATO preparation available in Hong Kong and is primarily adopted for the treatment of APL. Compared to the IV preparation, oral ATO is absorbed into the bloodstream via the gastrointestinal tract, resulting in potentially variable bioavailability attributable to the first-pass metabolism in the liver. However, a previous study has revealed oral ATO can achieve systemic bioavailability comparable to that of IV ATO [11]. Similar to IV administration, orally absorbed ATO is methylated in the liver.
In addition to Arsenol, Realgar-Indigo naturalis formula (RIF) is another oral ATO formulation approved in mainland China for the treatment of APL [20]. RIF is a combination of four natural products: realgar, indigo naturalis, radix salviae miltiorrhizae, and radix pseudostellariae [21]. Realgar contains approximately 90% of tetra-arsenic tetra-sulfide (As4S4), an oral form of arsenic compound that was originally used as traditional Chinese medicine. RIF has demonstrated efficacy comparable to IV ATO in APL [6,22].

3. Mechanisms of Action of Arsenic Trioxide in Leukemia

APL is caused by a reciprocal chromosome translocation, t(15;17), that leads to the formation of the PML/RARα fusion oncoprotein. This fusion protein impedes the differentiation of hematopoietic progenitor cells by disrupting transcriptional control and interfering with PML nuclear bodies (NBs), which are the key regulators of senescence [23], resulting in blunting of p53 signaling and increase in self-renewal of myeloid progenitors.
ATO induces partial differentiation and apoptosis in the APL cells through various molecular mechanisms [24]. It has a strong affinity for PML-RARα [25] and after binding, it interacts with the small ubiquitin-like protein modifier (SUMO)-conjugating enzyme UBC9, resulting in enhancement of SUMOylation and proteasomal degradation of the fusion protein that drives the growth of APL cells [26]. Degradation of PML-RARα restores biogenesis of PML NBs, leading to induction of senescence of the APL cells [27] (Figure 1).
On the other hand, ATO can induce either apoptotic cell death or partial differentiation, depending on the concentration used. At low concentrations (0.1-0.5µmol/L), ATO fosters differentiation of APL cells. However, at higher concentrations (0.5-2µmol/L), it activates the intrinsic apoptotic cell death pathway [28].
In addition, the in vitro anti-cancer and anti-angiogenic activities of ATO have been demonstrated in cell lines of liver, pancreatic and prostatic cancers [29,30,31]. It is hypothesized that ATO reduces production of angiogenic cytokines and induces apoptosis of tissue and endothelial cells [32].

4. Anti-Inflammatory Effects of Arsenic Trioxide

The multiple effects of ATO on cellular apoptosis, autophagy, differentiation, growth, angiogenesis, cytokine production and functions of the immune cells have extended its potential clinical use in other conditions beyond hematological malignancies. There is increasing evidence of the anti-inflammatory effects of ATO in murine models of systemic lupus erythematosus, systemic sclerosis and rheumatoid arthritis [33,34,35,36,37,38,39,40,41,42,43,44]. These are summarized in Table 1.

4.1. Systemic Lupus Erythematosus

The MRL/lpr lupus mice spontaneously develop a lupus-like syndrome that includes skin lesions, glomerulonephritis and vasculitis due to inactivation of Fas-mediated apoptosis. Peritoneal injection of these mice with ATO caused a rapid and significant reduction in the size of the spleen and lymph nodes, and suppressed skin lesions as compared to the control mice [33]. Autoantibody production, cytokines and immune complex deposition in the kidney was also reduced. Furthermore, ATO administration delayed the onset of nephritis and significantly improved the survival of these mice through activation of caspases and elimination of activated T cells responsible for lymphoproliferation and the lesions in skin, lung and the kidneys [33].
The lupus prone BXSB mice develop symptoms mimicking human SLE that is associated with autoantibody production, lymphoid activation and organ hyperplasia. A study evaluating the effects of tetra-arsenic tetra-sulfide (As4S4), another arsenic preparation, on the BXSB mice was performed. Treatment of these mice with As4S4 for 8 weeks resulted in reduction of splenomegaly and alleviation of skin, liver and kidney lesions with mild side effects. Histological analysis showed decreased immune complex deposition, mesangial proliferation, and inflammatory cell infiltration in the kidney and liver tissues. Moreover, As4S4 treatment also led to inhibition of monocytosis in the spleen and decreased serum interleukin-6 (IL-6) level [34].
In another in vitro experiment, the effect of different concentrations of ATO on IFN-γ expression in the splenocytes of MRL/lpr mice and peripheral blood mononuclear cells (PBMCs) of human SLE patients was investigated [35]. ATO treatment was effective in reducing the mRNA and protein expression levels of IFN-γ in the mice splenocytes and human SLE PBMCs, which was accompanied by a reduction in histone H4 and H3 acetylation in the IFN-γ promoter and decreased combination of RNA polymerase II to the IFN-γ promoter.

4.2. Systemic Sclerosis

In a murine model of hypochlorite-induced systemic sclerosis (SSc), daily intraperitoneal injections of ATO for 6 weeks limited dermal thickness and inhibited collage deposition as assessed by histological examination [36]. ATO treatment reduced vascular cell adhesion molecule 1 level and inhibited the autoantibody, IL-4 and IL-13 production by activated T cells. These beneficial effects of ATO were mediated through reactive oxygen species (ROS) generation that selectively killed activated fibroblasts. In an experimental model of chronic graft-versus-host disease (GVHD) induced by body radiation, followed by bone marrow and spleen cell transplantation, mice developed severe clinical symptoms that included diarrhea, alopecia, vasculitis, and fibrosis of the skin and internal organs [37]. Daily intraperitoneal injection of ATO to the mice abrogated these symptoms. The positive effects of ATO in this GVHD model were mediated by elimination of activated CD4+ T cells and plasmacytoid dendritic cells (pDCs) through depletion of glutathione [37].
The pDCs are a unique subset of dendritic cells that secret high levels of type I IFNs that are important in the pathophysiology of SSc and SLE. High concentrations of ATO were shown to induce apoptosis of pDCs derived from untreated patients with SSc via the mitochondrial pathway [38]. At clinically relevant concentrations, ATO preferentially inhibited IFN-α secretion and phosphorylation of the interferon regulatory factor 7. In addition, the capacity of pDCs to induce CD4+ T cell proliferation, Th1/Th2 polarization and B cell differentiation to plasmablasts was also downregulated by ATO administration.
In the hypochlorite-induced SSc murine model, copper ions combined with ATO significantly reduced skin thickening and cutaneous fibrosis in a manner equivalent to monotherapy with a double dose of ATO to induce the same immunological effects, which included a decrease in the number of B cells and reduced activation of CD4+ T cells [40]. Moreover, co-treatment of copper and ATO increased ROS production and enhanced apoptosis of murine fibroblasts compared to ATO alone.
The Fra2 transgenic mice (Fra2TG) mimics human SSc because they develop severe vascular remodeling of the pulmonary arterioles (pulmonary hypertension) and interstitial pneumonitis-like lung disease. A study showed that ATO treatment of these mice, as opposed to control mice, resulted in improvement of the lung histology and reduction of CD4+ T cell infiltration, a trend of reduction in fibrotic markers and a strong reduction in vascular remodelling [39]. RNA-sequencing analysis of lung tissues from ATO-treated mice revealed a downregulation of biological pathways associated with activity of the immune pathways, such as T-cell activation, regulation of leucocyte activation, leucocyte cell-cell adhesion, and regulation of lymphocyte activation.

4.3. Inflammatory Arthritis

In addition, ATO has been tested in the murine models of rheumatoid arthritis (RA). Fibroblast-like synoviocytes (FLS) are important cells that perpetuate inflammation in the joints and lead to structural damage. In a murine model of collagen-induced arthritis (CIA), ATO significantly enhanced the apoptosis of the (FLS) and contributed to histological recovery in the synovial membrane, along with inhibition of synovial hyperplasia and inflammation in the joints [41]. ATO may also alleviate inflammatory arthritis through the modulation of the balance in the Th17/Treg and Th1/Th2 pathways [42,43]. In an experiment, peripheral blood and synovial mononuclear cells were isolated from treatment naïve RA patients. In vitro administration of ATO resulted in the inhibition of Th17 differentiation through a reduction of STAT3 mRNA expression and enhancement of Treg cell generation through an augmentation of Foxp3 expression in these cells [34]. ATO was also shown to downregulate the Th1/Th2 ratio [34] and improve the Th17/Treg balance in the CIA mice [45]. The same group of investigators also demonstrated by single cell RNA-sequencing that ATO modulated several genes associated with inflammation, activation and differentiation of the peripheral blood Treg cells of treatment naïve RA patients [42,43].
More recently, there is evidence that ATO ameliorates CIA by altering the gut microbiota. Fecal samples from CIA and control mice were collected for 16S rDNA gene sequencing and metabolomic analysis [44]. Compared with control mice, CIA mice showed differences in the composition of gut microbiota in both the phylum and genus level, as well as in many metabolites, including benzoic acid and (s)-2-acetolactate. These alterations were partially reversed in by ATO treatment in the CIA mice, indicating that modulation of the gut microbiota and improvement in fecal metabolite abnormalities may be one mechanism of ATO in inflammatory arthritis.

5. Prospect of Arsenic Trioxide Treatment for SLE

5.1. Unmet Needs in the Treatment of SLE

Despite the improved life expectancy of patients with SLE in the few decades, further improvement in survival is hindered by the lack of blockbuster therapies in SLE [46]. Meta-analyses have shown an increase of the survival rates of SLE and lupus nephritis from the 1950s’ to 1990s’ but these plateaued off after the mid-1990s’ [47,48]. In low/middle-income countries, the 5-year and 10-year survival is still suboptimal. Refractory manifestations and treatment-related complications, especially those related to glucocorticoids (GCs), are the major causes of organ damage, mortality and impaired quality of life in SLE patients [49].
Two novel biological agents have recently been introduced for the treatment of SLE. They are belimumab, a monoclonal antibody against B cell activation factor (BAFF) [50,51] and anifrolumab, a monoclonal antibody against the type I interferon receptors [52,53]. Data beyond 4 years showed efficacy of both agents in reducing disease flares and organ damage, as well as achieving a GC sparing effect [54,55]. However, only belimumab is indicated for lupus nephritis and the effect size of having an increased renal response in addition to the standard of care (SOC) is not impressively high. Thus, the early use of biologics as upfront combination therapy in SLE is still controversial [56]. Moreover, the availability and reimbursement of these biological agents in less affluent countries remains problematic. As a result, there is an unmet need to develop new treatment strategies with an enhanced efficacy-to-toxicity ratio and cost-effectiveness in patients with SLE.

5.2. Disease Modifying Effects of SLE Therapies

Although disease modification has long been the concept of therapies of rheumatic diseases, its definition in SLE has not been well established. An international taskforce has suggested a conceptual framework to define the disease modifying effect of a treatment modality in SLE. These include: [1] improvement in disease activity of SLE with minimal treatment-associated toxicity; the absence of major flares; and a reduction in dosages of GC and immunosuppressive drugs during the first year of treatment; [2] continued improvement in disease activity of SLE, immunosuppression-sparing effect and the absence of flares during year 2-5 after therapy; and [3] the absence of new/worsening organ damage, including eGFR decline by 30% or more, throughout treatment to beyond 5 years [57].
A recent review of 32 selected clinical trials and 54 observational studies showed that 8 out of 14 SLE medications across different therapeutic classes met at least one of the criteria for disease modification up to 5 years [58]. While hydroxychloroquine improved overall survival beyond 5 years, no data on specific organ systems were reported. Only hydroxychloroquine and belimumab met disease modification definitions across three time points and beyond 5 years.

5.3. Novel Small Molecules for SLE

Novel small molecules targeting specific intracellular mechanisms of immune cells are being developed for the treatment of SLE [59]. The Janus kinases (JAKs), Bruton’s tyrosine kinases (BTKs) and spleen tyrosine kinases (SYKs) are important enzymes for activating receptor-mediated downstream signals from cytokines, growth factors, hormones and Fc/CD40/B-cell receptors [60]. Inhibition of these kinases impairs cellular activation and differentiation, leading to diminished actions of the inflammatory cytokines. Modulation of intracellular protein degradation in immunoproteasomes leads to depletion of long-lived plasma cells and reduced production of interferon (IFN)-α and autoantibodies. Interference of the sphingosine 1-phosphate (S1P)/S1P receptor-1 (S1PR1) pathway limits trafficking of autoreactive lymphocytes, enhances Treg functions and leads to decreased production of autoantibodies and the type I IFNs.
Although these targeted molecules are orally active and have the advantages of administration convenience and higher acceptance, lower production cost and the absence of immunogenicity, they are still in the experimental stages, and none has been approved for the treatment of SLE yet. Moreover, phase II trials of some of the JAK, BTK and SYK inhibitors in SLE were either negative or prematurely terminated [59]. Recent large phase III trials of baricitinib in non-renal SLE also failed to show clinical benefits [61,62]. While we are waiting for the results of ongoing phase III trials of other small molecules with promising phase II data (eg. deucravacitinib, zetomipzomib and cenerimod [59,63] exploration of alternative novel oral drugs for SLE is of paramount importance. The availability of oral forms of ATO and their preliminary benefits in the murine models of SLE warrants further attention in human SLE disease.

5.4. Preliminary Clinical Data of ATO in SLE

Clinical studies of ATO in rheumatic diseases are generally lacking despite the promising mechanisms of the drug in preclinical and murine studies. Recently, a pilot 24-week phase IIa trial from France has shown an acceptable safety profile of IV ATO (0.1-0.2mg/kg for 10 doses) in 11 patients with refractory SLE [64]. Four serious AEs occurred (grade 3 neutropenia, osteitis, neuropathy), 2 of which were related to ATO (neutropenia in 2 patients treated with higher doses of ATO and concomitant mycophenolate mofetil), leading to discontinuation of ATO. Neutrophil count recovered in these two patients without infective complications. Eighteen mild to moderate AEs were reported, which included hypomagnesemia, hypokalemia, diarrhea and asymptomatic moderate QT prolongation (<470 msec). Regarding efficacy, half of the patients achieved the SLE response index-4 (SRI-4) and the mean corticosteroid dosage was decreased from 11.3mg/day at baseline to 6mg/day at week 24. Six patients achieved SLE low disease activity state at week 52, which persisted at last follow-up (median 3 years, range 2-4 years). Titers of anti-dsDNA showed a trend of reduction after ATO therapy but levels of C3/4 and immunoglobulins were not altered. Pharmacokinetic study did not show correlation between plasma ATO levels and safety or efficacy.
As oral ATO has a better safety profile than the IV preparation and there are still no published studies of oral ATO in SLE, further clinical studies in SLE are necessary to establish safety and efficacy of this drug. SLE patients with non-organ threatening disease that is refractory to standard conventional therapies are ideal candidates for a new placebo-controlled randomized controlled trial.

5.5. Clinical Experience of ATO in Other Rheumatic Diseases

To our knowledge, ATO has not been clinically tested in other human rheumatic diseases other than SLE [64] as described above. In a report of 17 patients with APL and concomitant skin psoriasis, ATO-based induction regimen resulted in improvement of skin lesions in more than 80% of patients over a median follow-up period of 27 months [65]. The efficacy of oral ATO on skin psoriasis and psoriatic arthritis has to be further explored.

6. Conclusions

Arsenic and its compounds are historically known to be both a poison and a medicine. IV ATO has been used for the treatment of leukemia since the eighteenth century, but further development is hindered by its cardiac toxicities. With the introduction of an oral form of ATO, which is associated with a more favorable toxicity profile, it has become part of the standard therapies for APL. The anti-inflammatory properties of ATO in various murine models of rheumatic diseases also suggest its potential use in the treatment of human autoimmune diseases. In particular, the ex-vivo and in-vitro effects of ATO on modulation of Treg cell activation, depletion of activated T cells and dendritic cells, regulation of Th1/Th2 and Th17/Treg balance, reduction in autoantibody and cytokine production and alteration of the gut microbiota, as well as the benefits observed from murine studies indicate that ATO worth further exploration in human SLE. Preliminary experience in refractory SLE revealed efficacy of IV ATO with acceptable safety profile. As the oral preparation of ATO is associated with even fewer toxicities, further clinical trials of this drug in non-organ threatening SLE are eagerly awaited.

Conflicts of Interest

The authors declare no conflicts of interest.

Abbreviations

PML=promyelocytic leukemia;
RARα=retinoic acid receptor alpha;
NB=nuclear bodies;
ATO=arsenic trioxide;
SUMO= small ubiquitin-like protein modifier;
ATRA=all-trans retinoic acid

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Preprints 114931 g001
Figure 1. Mechanism of action of arsenic trioxide in promyelocytic leukemia.1.Translocation (t15:17) leads to formation of the PML/RARα fusion oncoprotein; 2. This protein impedes the differentiation of myeloid progenitors by interfering with PML NBs; 3. ATO binds to PML-RARα and enhances SUMOylation and proteasomal degradation of PML/RARα; 4. Degradation of PML-RARα restores biogenesis of PML NBs, induces senescence of the APL cells and restores terminal differentiation of the myeloid progenitors.
Figure 1. Mechanism of action of arsenic trioxide in promyelocytic leukemia.1.Translocation (t15:17) leads to formation of the PML/RARα fusion oncoprotein; 2. This protein impedes the differentiation of myeloid progenitors by interfering with PML NBs; 3. ATO binds to PML-RARα and enhances SUMOylation and proteasomal degradation of PML/RARα; 4. Degradation of PML-RARα restores biogenesis of PML NBs, induces senescence of the APL cells and restores terminal differentiation of the myeloid progenitors.
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Table 1. Immune mechanisms of arsenic trioxide in autoimmune rheumatic conditions.
Table 1. Immune mechanisms of arsenic trioxide in autoimmune rheumatic conditions.
Authors, years Model Clinical effects Immunological effects
Bobe et al., 2006 [33] Lupus mice ↓ lymphoproliferation, skin, lung and kidney inflammation; significantly prolonged survival Induced apoptosis and depletion of auto-reactive T cells, ↓ production of autoantibodies and cytokines
Zhao et al., 2013 [34] Lupus mice ↓splenomegaly, amelioration of skin, liver and renal lesions ↓ immune complex deposition, mesangial proliferation, and inflammatory cell infiltration in kidney and liver tissues, ↓monocytosis in spleen and serum interleukin-6 level
Hu et al., 2018 [35] Lupus mice and blood cells from SLE patients In vitro experiments ↓mRNA and protein expression of IFN-γ in mice splenocytes and human SLE PBMCs
Kavian et al., 2012 [36] Hypochlorite induced SSc mice ↓dermal thickness and collagen deposition in skin and lung tissues ↓vascular cell adhesion molecule 1 level, autoantibody, IL-4 and IL-13 production by activated T cells; selectively killed activated fibroblasts through ROS generation
Kavian et al., 2012 [37] Induced chronic GVHD in mice ↓GVHD symptoms, fibrosis of skin and internal organs ↓activated CD4+ T cells and plasmacytoid dendritic cells (pDCs) through depletion of glutathione
Ye et al., 2020 [38] Blood cells from SSc patients In vitro experiments Induced apoptosis of pDCs, preferentially inhibited IFN-α secretion and phosphorylation of the interferon regulatory factor 7, ↓capacity of pDCs to induce CD4+ T cell proliferation, Th1/Th22 polarization and B cell differentiation to plasmablasts
Cauvet et al., 2023 [39] Pre-clinical SSc mice Improvement in lung histology, trend of reduction in fibrosis markers and strong reduction in vascular remodeling ↓memory T cells, ↑% of naive T cells in the lungs; downregulated biological pathways associated with activity of the immune pathways, such as T-cell activation, regulation of leucocyte activation, leucocyte cell-cell adhesion, and regulation of lymphocyte activation.
Chene et al., 2023 [40] Hypochlorite induced SSc mice ↓skin thickening and fibrosis ↓number of B cells and activation of CD4+ T cells, ↑ROS production and apoptosis of murine fibroblasts
Mei et al., 2011 [41] Collagen induced arthritis in mice ↓synovial hyperplasia and inflammation in the joints Enhanced apoptosis of fibroblast-like synoviocytes
Li et al., 2019 [42] Blood and synovial cells from RA patients In vitro experiments ↓Th17 differentiation through a reduction of STAT3 mRNA expression, ↑Treg cell generation through an augmentation of Foxp3 expression, downregulated the Th1/Th2 ratio
Li et al., 2021 [43] Treg cells from early RA patients In vitro experiments Modulated expression of several genes associated with inflammation, Treg-cell activation and differentiation
Niu et al., 2022 [44] Collagen-induced arthritis in mice ↓arthritis Modulated gut microbiota and improved fecal metabolite abnormalities
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