High Sensitivity Detection of Oral Leukoplakia Comprehensive Analysis of P90 Biomarker Expression in Human Saliva and Tissue Samples

Hsiao-Hsuan Wan; Haochen Zhu; Chao-Ching Chiang; Jian-Sian Li; Fan Ren; Cheng-Tse Tsai; Yu-Te Liao; Dan Neal; Joseph Katz; Josephine F. Esquivel-Upshaw

doi:10.20944/preprints202404.1831.v1

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preprints.org > public health and healthcare > public health and health services > doi: 10.20944/preprints202404.1831.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

High Sensitivity Detection of Oral Leukoplakia Comprehensive Analysis of P90 Biomarker Expression in Human Saliva and Tissue Samples

Josephine F. Esquivel-Upshaw

Version 1 : Received: 26 April 2024 / Approved: 28 April 2024 / Online: 28 April 2024 (08:08:43 CEST)

How to cite: Wan, H.; Zhu, H.; Chiang, C.; Li, J.; Ren, F.; Tsai, C.; Liao, Y.; Neal, D.; Katz, J.; Esquivel-Upshaw, J.F. High Sensitivity Detection of Oral Leukoplakia Comprehensive Analysis of P90 Biomarker Expression in Human Saliva and Tissue Samples. Preprints 2024, 2024041831. https://doi.org/10.20944/preprints202404.1831.v1 Wan, H.; Zhu, H.; Chiang, C.; Li, J.; Ren, F.; Tsai, C.; Liao, Y.; Neal, D.; Katz, J.; Esquivel-Upshaw, J.F. High Sensitivity Detection of Oral Leukoplakia Comprehensive Analysis of P90 Biomarker Expression in Human Saliva and Tissue Samples. Preprints 2024, 2024041831. https://doi.org/10.20944/preprints202404.1831.v1

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MDPI and ACS Style

Wan, H.; Zhu, H.; Chiang, C.; Li, J.; Ren, F.; Tsai, C.; Liao, Y.; Neal, D.; Katz, J.; Esquivel-Upshaw, J.F. High Sensitivity Detection of Oral Leukoplakia Comprehensive Analysis of P90 Biomarker Expression in Human Saliva and Tissue Samples. Preprints 2024, 2024041831. https://doi.org/10.20944/preprints202404.1831.v1

APA Style

Wan, H., Zhu, H., Chiang, C., Li, J., Ren, F., Tsai, C., Liao, Y., Neal, D., Katz, J., & Esquivel-Upshaw, J.F. (2024). High Sensitivity Detection of Oral Leukoplakia Comprehensive Analysis of P90 Biomarker Expression in Human Saliva and Tissue Samples. Preprints. https://doi.org/10.20944/preprints202404.1831.v1

Chicago/Turabian Style

Wan, H., Joseph Katz and Josephine F. Esquivel-Upshaw. 2024 "High Sensitivity Detection of Oral Leukoplakia Comprehensive Analysis of P90 Biomarker Expression in Human Saliva and Tissue Samples" Preprints. https://doi.org/10.20944/preprints202404.1831.v1

Abstract

Oral cancer represents a significant global public health challenge, contributing substantially to cancer incidence and mortality. Despite established risk factors such as tobacco use and alcohol consumption, early detection remains crucial for effective treatment. This study introduces a novel approach using a transistor-based biosensor system for detecting the P90 (CIP2A) protein. We have tested the presence of CIP2A in human leukoplakia samples that can rise into oral squamous cell carcinoma. The method employs commercially available glucose test strips functionalized with P90 antibodies, providing high sensitivity and a low limit of detection, which is five orders lower than commercial ELISA kits. A specially designed printed circuit board (PCB) facilitates accurate measurements, and the device's performance is optimized through characteristic tests. Human sample testing validates the biosensor's effectiveness in distinguishing samples after cell lysis. This study contributes to advancing accurate and cost-effective diagnostic approaches for oral pre-cancer and cancer tissues.

Keywords

oral cancer detection; oral leukoplakia; P90; high sensitivity; salivary biomarkers; MOSFET; tissue

Subject

Public Health and Healthcare, Public Health and Health Services

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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