Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Genomic Characterization of Carbapenemase-Producing Enterobacter hormaechei, Serratia marcescens, Citrobacter freundii, Providencia stuartii and Morganella morganii Clinical Isolates from Bulgaria

Stefana Sabtcheva
* ORCID logo ,
Ivan Stoikov
ORCID logo ,
Ivan N. Ivanov
ORCID logo ,
Deyan Donchev
,
Magdalena Lesseva
,
Sylvia Georgieva
,
Deana Teneva
,
Elina Dobreva
,
Iva Christova
Version 1 : Received: 26 April 2024 / Approved: 26 April 2024 / Online: 28 April 2024 (08:17:31 CEST)

How to cite: Sabtcheva, S.; Stoikov, I.; Ivanov, I.N.; Donchev, D.; Lesseva, M.; Georgieva, S.; Teneva, D.; Dobreva, E.; Christova, I. Genomic Characterization of Carbapenemase-Producing Enterobacter hormaechei, Serratia marcescens, Citrobacter freundii, Providencia stuartii and Morganella morganii Clinical Isolates from Bulgaria. Preprints 2024, 2024041794. https://doi.org/10.20944/preprints202404.1794.v1 Sabtcheva, S.; Stoikov, I.; Ivanov, I.N.; Donchev, D.; Lesseva, M.; Georgieva, S.; Teneva, D.; Dobreva, E.; Christova, I. Genomic Characterization of Carbapenemase-Producing Enterobacter hormaechei, Serratia marcescens, Citrobacter freundii, Providencia stuartii and Morganella morganii Clinical Isolates from Bulgaria. Preprints 2024, 2024041794. https://doi.org/10.20944/preprints202404.1794.v1

Abstract

Carbapenemase-producing Enterobacter spp. Serratia marcescens, Citrobacter freundii, Providencia spp. and Morganella morganii (CP-ESCPM) are increasingly identified as causative agents of nosocomial infections but are still not under systematic genomic surveillance. In this study, using a combination of whole genome sequencing and conjugation experiments, we sought to elucidate the genomic characteristics and transferability of resistance genes in clinical CP-ESCPM isolates from Bulgaria. Among the 36 sequenced isolates, NDM-1 (12/36), VIM-4 (11/36), VIM-86 (8/36) and OXA-48 (7/36) carbapenemases were identified; two isolates carried both NDM-1 and VIM-86. The majority of carbapenemase genes were found on self-conjugative plasmids. IncL plasmids were responsible for the spread of OXA-48 among E. hormaechei, C. freundii and S. marcescens. IncM2 plasmids were generally associated with the spread of NDM-1 in C. freundii and S. marcescens, and also of VIM-4 in C. freundii. IncC plasmids were involved in the spread of the recently described VIM-86 in P. stuartii isolates. IncC plasmids carrying blaNDM-1 and blaVIM-86 have been observed too. blaNDM-1 was also detected on IncX3 in S. marcescens and on IncT plasmid in M. morganii. The significant resistance transfer rates highlight the role of the ESCPM group as a reservoir of resistance determinants and stress the need for strengthening the infection control measures.

Keywords

carbapenemases; NDM-1; VIM-4; VIM-86; OXA-48; horizontal gene transfer; Enterobacter; Serratia; Citrobacter; Providencia; Morganella

Subject

Medicine and Pharmacology, Medicine and Pharmacology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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