PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
A Acylhydrazone Compound A5 Promotes Neurogenesis by Up-regulating Neurogenesis-related Genes and Inhibiting Cell-cycle Progression in Neural Stem/Progenitor Cells
Version 1
: Received: 24 April 2024 / Approved: 24 April 2024 / Online: 24 April 2024 (10:07:46 CEST)
How to cite:
Xiang, X.; Jiang, X.; Lin, H.; Yu, M.; Wu, L.; Zhou, R. A Acylhydrazone Compound A5 Promotes Neurogenesis by Up-regulating Neurogenesis-related Genes and Inhibiting Cell-cycle Progression in Neural Stem/Progenitor Cells. Preprints2024, 2024041604. https://doi.org/10.20944/preprints202404.1604.v1
Xiang, X.; Jiang, X.; Lin, H.; Yu, M.; Wu, L.; Zhou, R. A Acylhydrazone Compound A5 Promotes Neurogenesis by Up-regulating Neurogenesis-related Genes and Inhibiting Cell-cycle Progression in Neural Stem/Progenitor Cells. Preprints 2024, 2024041604. https://doi.org/10.20944/preprints202404.1604.v1
Xiang, X.; Jiang, X.; Lin, H.; Yu, M.; Wu, L.; Zhou, R. A Acylhydrazone Compound A5 Promotes Neurogenesis by Up-regulating Neurogenesis-related Genes and Inhibiting Cell-cycle Progression in Neural Stem/Progenitor Cells. Preprints2024, 2024041604. https://doi.org/10.20944/preprints202404.1604.v1
APA Style
Xiang, X., Jiang, X., Lin, H., Yu, M., Wu, L., & Zhou, R. (2024). A Acylhydrazone Compound A5 Promotes Neurogenesis by Up-regulating Neurogenesis-related Genes and Inhibiting Cell-cycle Progression in Neural Stem/Progenitor Cells. Preprints. https://doi.org/10.20944/preprints202404.1604.v1
Chicago/Turabian Style
Xiang, X., Liming Wu and Rong Zhou. 2024 "A Acylhydrazone Compound A5 Promotes Neurogenesis by Up-regulating Neurogenesis-related Genes and Inhibiting Cell-cycle Progression in Neural Stem/Progenitor Cells" Preprints. https://doi.org/10.20944/preprints202404.1604.v1
Abstract
Adult neurogenesis involves generation of functional neurons from neural progenitor cells, which have the potential to complement and restore damaged neurons and neural circuits. Therefore, the development of drugs that stimulate neurogenesis represents a promising strategy in stem cell therapy and neural regeneration, greatly facilitating the reconstruction of neural circuits in cases of neurodegeneration and brain injury. Our study reveals that compound A5, previously designed and synthesized by our team, exhibits remarkable neuritogenic activities, effectively inducing neurogenesis in neural stem/progenitor cells (NSPCs). Subsequently, transcriptome analysis using high-throughput Illumina RNA-seq technology was performed to further elucidate the underlying molecular mechanisms by which compound A5 promotes neurogenesis. Notably, comparative transcriptome analysis showed that the up-regulated genes were mainly associated with neurogenesis and the down-regulated genes were mainly concerned with cell cycle progression. Furthermore, we confirmed that compound A5 significantly affected the expression of transcription factors related to neurogenesis and cell cycle regulatory proteins. Collectively, these findings identify a new compound with neurogenic activity and may provide insights into drug discovery for neural repair and regeneration.
Medicine and Pharmacology, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
