Version 1
: Received: 23 April 2024 / Approved: 23 April 2024 / Online: 24 April 2024 (09:57:15 CEST)
How to cite:
Steinmetz, E.L.L.; Scherer, A.; Calvet, C.; Müller, U. Orthologs of NOX5 and EC-SOD/SOD3: dNox and dSod3 Impact Egg Hardening Process and Egg Laying in Reproductive Function of Drosophila melanogaster. Preprints2024, 2024041588. https://doi.org/10.20944/preprints202404.1588.v1
Steinmetz, E.L.L.; Scherer, A.; Calvet, C.; Müller, U. Orthologs of NOX5 and EC-SOD/SOD3: dNox and dSod3 Impact Egg Hardening Process and Egg Laying in Reproductive Function of Drosophila melanogaster. Preprints 2024, 2024041588. https://doi.org/10.20944/preprints202404.1588.v1
Steinmetz, E.L.L.; Scherer, A.; Calvet, C.; Müller, U. Orthologs of NOX5 and EC-SOD/SOD3: dNox and dSod3 Impact Egg Hardening Process and Egg Laying in Reproductive Function of Drosophila melanogaster. Preprints2024, 2024041588. https://doi.org/10.20944/preprints202404.1588.v1
APA Style
Steinmetz, E.L.L., Scherer, A., Calvet, C., & Müller, U. (2024). Orthologs of NOX5 and EC-SOD/SOD3: dNox and dSod3 Impact Egg Hardening Process and Egg Laying in Reproductive Function of Drosophila melanogaster. Preprints. https://doi.org/10.20944/preprints202404.1588.v1
Chicago/Turabian Style
Steinmetz, E.L.L., Célestine Calvet and Uli Müller. 2024 "Orthologs of NOX5 and EC-SOD/SOD3: dNox and dSod3 Impact Egg Hardening Process and Egg Laying in Reproductive Function of Drosophila melanogaster" Preprints. https://doi.org/10.20944/preprints202404.1588.v1
Abstract
The occurrence of ovarian dysfunction is often due to the imbalance between the formation of reactive oxygen species (ROS) and the (in)effectiveness of the antioxidative defense mechanisms. Primary sources of ROS are the respiratory electron transfer and the activity of NADPH oxidases (NOX), while Superoxide dismutases (SOD) are the main key reg-ulators that control the levels of ROS and reactive nitrogen species intra- and extracel-lularly. Because of their central role, SODs are the subject of research on human ovarian dysfunction, but the sample acquisition is low. The high degree of cellular and molecular similarity between Drosophila melanogaster ovaries and human ovaries provides this model organism with the best conditions for analyzing the role of ROS during ovarian function. In this study, we clarify the localization of the ROS producing enzyme dNox within ovaries of Drosophila melanogaster and by a tissue specific knockdown, we show that dNox derived ROS are involved in the chorion hardening process. Furthermore, we analyze the dSod3 localization and show that reduced activity of dSod3 impacts egg laying behavior, but not the chorion hardening process.
Keywords
NADPH oxidase 5 (NOX5); (extracellular) superoxide dismutase (EC-SOD or SOD3)
Subject
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
