3.1. Regional Characterization
The study area encompasses lands of four municipalities around the estuary of the Sado river, within the boundaries of the hydrographic basin - Setúbal, Palmela, Alcácer do Sal, and Grândola (
Figure 1). The choice of this area is justified by its highly dynamic environment, where natural factors and phenomena interact, alongside with growing urban expansion and economic activities.
The downstream limit of the study area corresponds to the river mouth of the Sado, a line defined by the Portuguese Official Administrative Chart (CAOP), between the Tróia peninsula and the city of Setúbal. The upstream limit has been considered as the upper tip of the higher dynamic tides, situated about 15 km SE of the city of Alcácer do Sal [
14].
The Sado hydrographic basin drains an area of 7,692 km², making it the largest entirely Portuguese basin. It is delimited to the north by the Tagus basin, to the east by the Guadiana basin, to the south by the Mira basin, and to the west by a coastal strip that drains directly into the sea [
15]. The Sado river originates in Ourique, in the Serra da Vigia (230 high), and flows into the Atlantic Ocean, with a total length of 180 km. The estuary has a submerged area of approximately 200 km², corresponding to the estuarine basin and the submarine ebb delta [
16].
The area falls within the Mediterranean temperate climate zone, with hot, dry summers and an average annual precipitation of around 600 mm [
16]. Rainfall is concentrated in the autumn-winter period, with 60% of the annual volume, exceeding 70 mm/month from October to January.
The geological basement includes different lithologies and ages, from the Paleozoic to the Quaternary period predominate. The main river and its tributaries drain mostly Cenozoic terrigenous deposits of the Sado Basin [
17]. This basin is bounded: I) to the north, by the Arrábida Mountain Range consisting of Mesozoic carbonate rocks; ii) to the east, by a morphological rise, marking the contact between the Cenozooic sedimentary rocks and the Paleozoic mostly metamorphic rocks of the South Portuguese Zone (SPZ) and the Ossa-Morena Zone (OMZ); iii) to the west, by the Atlantic Ocean, and iv) to the southwest, by the Grândola fault [
15].
The geomorphology is characterized by a flat and gently undulating landscape, with a low gradient slope and very small elevations, except for the mountain ranges of Arrábida and Grândola. The predominant soils have a sandy texture due to the rocks and deposits that originated them.
Currently, according to the 2021 demographic census, the resident population in the municipalities corresponds to about 217 thousand inhabitants, engaged in rural and urban activities, port operations, industrial activities, agroforestry production, and tourism. Setúbal has the highest population density an both Setúbal and Palmela showed growth rates, while Alcácer do Sal and Grândola experienced a population decline in 2021, compared to the previous census.
3.2. Historical Land Use and Occupation
Land use and occupation patterns reveal how societies manage landscape and use its natural resources. Human interventions tend to produce imbalances or environmental impacts, proportional to their technological development.
Human presence around the Sado estuary dates back to the Mesolithic period [
18]. Approximately 20 thousand years ago, global warming led to the melting of ice in the Northern Hemisphere, causing sea levels to rise and marine transgressions resulted in the flooding of lower river sectors, forming estuaries [
19].
The mild Holocene temperatures favoured the migration of Mesolithic peoples to coastal regions. Due to ecological-geographical characteristics, the Sado estuary was occupied by these peoples, who lived in the area exploiting food resources [
20]. Holocene climatic fluctuations in Portuguese territory modified the original land cover, particularly in the coastal region, where the sub-humid climate favoured the expansion of maritime pine forests (Pinus pinaster) and oak forests (Quercus) [
21].
Chalcolithic shell middens have been found in the Sado valley and its tributaries on river terraces and at the base of slopes, indicating the occurrence of sea-level rise and occupation of the area between approximately 8400-7000 cal BP [
18]. These peoples mainly lived of bivalve mollusk gathering, fishing, and hunting, and remained in this territory for about a thousand years.
Over time, other peoples inhabited the lands of the Sado river and estuary, including Romans, Moors, French, and others, interested in natural resources and/or their strategic geographical position. Archaeological studies have revealed the existence of Roman kilns dating from the 1st century BC to the 5th century AD, which produced amphorae in the locations of Abul and Herdade Pinheiro - Alcácer do Sal. The amphorae were intended for the industrial complex where fish salting was processed, in the Tróia peninsula [
21]. Despite ancient occupation and use of natural resources in this area, these peoples did not cause significant environmental changes.
Salt production was developed in Setúbal and Alcácer do Sal since the Roman period. In the 16th century, salt pans were built in marsh areas along the banks of the Sado, mainly in Abul, Batalha, Faralhões, and Gâmbia. In the following century, the activity expanded to the Mitrena peninsula. Salt production played a prominent role in the Portuguese economy until the end of the 19th century, when it began to decline [
22].
In the 19th-century map (
Figure 2), the main types of land use and cover are represented. At that time, most of the territory was occupied by heaths and scrublands - typical xerophytic vegetation covering extensive plains. These protected the low-fertility sandy soils from erosive processes by adding organic matter.
Rice cultivation has been established in the district of Setúbal, particularly in Alcácer do Sal, since the 18th century [
22]. By the end of the 19th century, rice and wheat, the predominant arable crops, thrived on the riverbanks. On the hill tops, historical documents also record the replacement of extensive areas of pine forests and heaths by extensive vineyards. Agroforestry use corresponded mainly to extensive "montados" of cork oak (Quercus suber) and holm oak (Quercus ilex), and to a lesser extent stone pine (Pinus pinea). This occupation occurred at the beginning of the Liberal Regime, the end of the 18th century, and the beginning of the 19th century [
23]. At the time, favourable factors for their establishment were large private landholdings, cheap wage labor, and state support for the removal of native vegetation.
In the 19th century, cork oak “montados” dominated the territory of Grândola, Águas de Moura-Palmela, and Alcácer do Sal. Pine trees covered the central sector of the study area, and holm oaks predominated in areas with dryer climatic conditions (
Figure 2). Saltpans dominated the plains affected by ocean tides, while rice cultivation developed in large plains flooded by river waters. In the northwest sector, in Palmela and Setúbal, horticultural crops and vineyards stood out, while in Alcácer do Sal, vineyards were intercropped with olive groves.
3.2. 21st Century Land Use and Occupation
Between the 19th century and the present day, the landscape of the Sado territory has undergone significant changes, with the emergence of new activities and the retraction of others. In this analysis, seven main classes of land use and cover were identified: 1) Artificial Surfaces - urban areas, industry, and commerce; 2) Agriculture and cultivable lands - viticulture, olive cultivation, rice cultivation, and mixed farming; 3) Pasture - native and planted forage; 4) Agroforestry and forest area - cork oak “montados”, holm oak, and pine trees; 5) Mixed Vegetation - agroforestry lands with different oak species and shrubs; 6) Coastal Environments - beaches and dune fields; 79 Coastal Wetlands - salt marshes, seagrass meadows, and intertidal zones (
Figure 3). The expansion of the urbanization process in Setúbal is striking in this century, clearly influenced by its proximity to Lisbon, the country's capital. Setúbal and Palmela are part of the Lisbon Metropolitan Area (LMA). Setúbal experiences solid economic development provided by port activity on the Mitrena peninsula, which has also contributed to urban growth (
Figure 4g).
In all municipalities, the areas of alluvial plains and part of the tidal plains are cultivated with different kinds of rice. Portugal is among the five largest producers (and also consumers per capita) of rice in Europe, and the municipalities of Alcácer do Sal and Grândola stand out at the national scale (
Figure 4b). Salt production has experienced a sharp decline, occurring in small areas of the tidal plain in the parishes of Faralhão and Gâmbia (
Figure 4a). Vineyards are increasing in the northwest region, in the municipalities of Setúbal and Palmela, part of the Arrábida Chain, providing renowned wine production in the national and international markets.
Pine forests have spread widely across the territory on the left bank of the Sado to meet the demands of the timber, furniture, pulp, and paper industries. Both maritime pine and stone pine have adapted to sandy and infertile soils. The increase in areas with maritime pine forests is justified for reducing wind erosion of the sandy soils and also of coastal dunes. Additionally, these pines produce pine nuts, an edible seed highly used in local gastronomy, especially in Alcácer do Sal.
The landscape is characterized by agro-silvo-pastoral systems represented by "montados," which combine cork oak or holm oak forests, agricultural crops, forage plants, and livestock farming – cattle or sheep (
Figure 4f). The cork oak provides raw material for the cork industry, ensuring Portugal's position as the world's largest cork producer (
Figures 4c and 4d). These systems have a complex management, being known by favouring landscape conservation/maintenance, soil nutrients, biodiversity, and water resources, as well as being a cultural trait of the large Alentejo region, southern Portugal’s extensive dry plains.
Among the activities identified in the area, limestone extraction for cement production is the most visually impacting on the landscape. This activity is concentrated in the Arrábida mountain range, which presents highly degraded areas, although it corresponds to a protected environmental unit - the Arrábida Natural Park.
Visual and noise impacts are high, affecting the resident population in the surrounding areas, and plans to enlarge the extraction area have recently been refused by local authorities (CCDR-LVT). The environmental impacts of this activity include the degradation of biotic and abiotic elements of the landscape – relief, soils, vegetation, river channels, habitat destruction, and loss of biodiversity- affecting the interrelationships between components of the environmental system. Although the companies have approved and ongoing plans for environmental restoration, mainly through reforestation, the imbalances disrupt the dynamics of the natural system, which once disrupted does not return to its original characteristics.
The Grândola mountain range also shows scars resulting from historical underground mining. For many years, the Caveira and Lousal mines were in operation, extracting ores from the Iberian Pyrite Belt rocks. Studies indicate that besides inheriting landscape degradation, there was also soil and water contamination by heavy metal residues [
24]. In Alcácer do Sal, the exploitation of geological resources (kaolin and quartz) occurs in the Casal Ventoso sandpit formed by dunes and deposits of Holocene aeolian sands [
25] and in Lagoa Salgada (
Figure 4e).
In the river channels of the Sado basin hydrographic network, the most frequent anthropogenic interventions are dams, affecting the flow dynamics of rivers and the volume of transported sediments. Thus, sedimentary processes in the estuary are also altered, with erosion points in the tidal plains, colonized by salt marshes.
In the current spatial organization of the area, the Sado Estuary Natural Reserve (RNES), an area created by law in 1980, stands out to ensure the conservation of the estuary and the development of activities compatible with this ecosystem [
26]. To achieve this, in 2008, the Reserve's Management Plan (PORNES) established the types of use for the National Ecological Reserve (REN) – areas not heavily urbanized or with biological interest, and for the National Agricultural Reserve (RAN) – lands with fertile soils or suitable for agricultural use [
23,
26]. The Directorate-General for Agriculture and Rural Development considers RAN a land management tool, recognizing its suitability for agriculture due to agroclimatic, geomorphological, and pedological characteristics. However, there is an overlap of RAN in some REN areas, as well as the use of parts of the tidal plain for rice and salt production (
Figure 3).
Tourism is developing on the coastal beaches of Grândola and in the Sado Estuary itself, where the only community of bottlenose dolphins lives. Historical Roman ruins in Tróia, the palafitic pier of Carvalhal, and the religious and military monuments of the Arrábida mountain range are also important local attractions.
Port and industrial activities in the Mitrena peninsula, require frequent dredging in the estuary to allow for the passage of deeper-draft ships, resulting in a permanent threat to estuarine biotopes. Increased water turbidity and contamination affect the seagrass meadows that sustain the estuarine food-chain, including the bottlenose dolphin community, while salt marshes and mudflat areas are also modified. The Institute for Nature Conservation and Forests recognizes that dredging can cause environmental problems and reduce salt marshes but understands that they are carried out downstream of RNES, i.e., outside the area included in PORNES.