Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Dual PPAR-α/γ Activation by Algal Fiber-Rich NBF2 Formula Reverses Dyslipidemia and Hyperglycemia In Vivo

Nlandu Roger Ngatu
* ORCID logo ,
Akram Hossain
,
Nao Mruo
,
Steeve Akumwami
,
Asadur Md Rahman
,
Masamitsu Eitoku
,
Kanae Kanda
ORCID logo ,
Luzitu Severin Nangana
,
Kuezina Daniel-Tonduangu
,
Akira Nishiyama
,
Narufumi Suganuma
,
Tomohiro Hirao
Version 1 : Received: 8 April 2024 / Approved: 9 April 2024 / Online: 9 April 2024 (11:40:48 CEST)

How to cite: Ngatu, N.R.; Hossain, A.; Mruo, N.; Akumwami, S.; Rahman, A.M.; Eitoku, M.; Kanda, K.; Nangana, L.S.; Daniel-Tonduangu, K.; Nishiyama, A.; Suganuma, N.; Hirao, T. Dual PPAR-α/γ Activation by Algal Fiber-Rich NBF2 Formula Reverses Dyslipidemia and Hyperglycemia In Vivo. Preprints 2024, 2024040632. https://doi.org/10.20944/preprints202404.0632.v1 Ngatu, N.R.; Hossain, A.; Mruo, N.; Akumwami, S.; Rahman, A.M.; Eitoku, M.; Kanda, K.; Nangana, L.S.; Daniel-Tonduangu, K.; Nishiyama, A.; Suganuma, N.; Hirao, T. Dual PPAR-α/γ Activation by Algal Fiber-Rich NBF2 Formula Reverses Dyslipidemia and Hyperglycemia In Vivo. Preprints 2024, 2024040632. https://doi.org/10.20944/preprints202404.0632.v1

Abstract

Fiber-rich green river Aonori alga is mass-cultivated in Japan; it has been reported to reduce inflammation and improve metabolic health in humans. Peroxisome proliferation-activated receptor (PPARs) are associated with metabolic disorders such as obesity and type 2 diabetes (T2DM). We evaluated the effects of supplementation with NBF2, a formula made of algal and junos citrus biomaterials, on metabolic markers in obese and diabetic rats. An experimental study was performed using 18 obese and hyperglycemic Otsuka Long-Evans Tokushima Fatty (OLETF) rats randomly assigned to high dose NBF2 (20mg/kg; n=6), low dose (10mg/kg; n=6) NBF2 drink for 6 weeks, whereas OLETF control rats received similar amount of tap water (vehicle; n=6). LETO rats were used as normal controls (n=8). Biospecimens were taken for ELISA and real-time PCR. Both high dose and low dose NBF2 increased brown adipose tissue (BAT) mass and adiponectin (APN), improved insulin sensitivity, and significantly improved glycemic and lipid (TG, LDL-c) profiles in NBF2-supplemented OLETF rats after a 6-week supplementation with NBF2 (vs. OLETF controls), as well as LDL-c (p<0.05). During the glucose tolerance test (OGTT), both NBF2 groups and LETO rats had normal glycemia by 90-minute time-point (vs. OLETF controls; p<0.01). Furthermore, intake of NBF2 up-regulated PPARa/g-mRNA and Sirt2-mRNA gene expressions in BAT, and improved blood pressure profile in OLETF rats. Findings from this research suggest that intake of NBF2 formula induces dual PPAR-a/g and Sirt2 activation, providing insights on beneficial prophylactic and therapeutic effects of this NB2 formula on dyslipidemia and hyperglycemia in T2DM.

Keywords

Algal formula; Dyslipidemia; Obese rat; Type 2 diabetes; Peroxisome proliferation-activated receptor; Type 2 diabetes

Subject

Medicine and Pharmacology, Complementary and Alternative Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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