Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Novel Dairy Fermentates Have Differential Effects on Key Immune Responses Associated with Viral Immunity and Inflammation

Dearbhla Finnegan
,
Monica A Mechoud
,
Claire Connolly
ORCID logo ,
Jamie A FitzGerald
ORCID logo ,
Tom Beresford
ORCID logo ,
Harsh Mathur
,
Lorraine Brennan
ORCID logo ,
Paul D. Cotter
ORCID logo ,
Christine Loscher
*
Version 1 : Received: 5 April 2024 / Approved: 5 April 2024 / Online: 7 April 2024 (22:43:55 CEST)

How to cite: Finnegan, D.; Mechoud, M.A.; Connolly, C.; FitzGerald, J.A.; Beresford, T.; Mathur, H.; Brennan, L.; Cotter, P.D.; Loscher, C. Novel Dairy Fermentates Have Differential Effects on Key Immune Responses Associated with Viral Immunity and Inflammation. Preprints 2024, 2024040455. https://doi.org/10.20944/preprints202404.0455.v1 Finnegan, D.; Mechoud, M.A.; Connolly, C.; FitzGerald, J.A.; Beresford, T.; Mathur, H.; Brennan, L.; Cotter, P.D.; Loscher, C. Novel Dairy Fermentates Have Differential Effects on Key Immune Responses Associated with Viral Immunity and Inflammation. Preprints 2024, 2024040455. https://doi.org/10.20944/preprints202404.0455.v1

Abstract

Fermented foods and ingredients, including fermentates derived from lactic acid bacteria (LAB) in dairy products, can modulate the immune system. Here we describe the use of reconstituted skimmed milk powder to generate novel fermentates from Lactobacillus helveticus strains SC232, SC234, SC212, and SC210, and from Lacticaseibacillus casei strains SC209 and SC229, and demonstrate using in vitro assays that these fermentates can differentially modulate cytokine secretion by bone marrow derived dendritic cells (BMDCs) when activated with either the viral ligand, loxoribine, or an inflammatory stimulus, lipopolysaccharide. Specifically, we demonstrate that SC232 and SC234 increase cytokines IL-6, TNF-α, IL-12p40, IL-23, IL-27 and IL-10, and decreased IL-1β in primary bone marrow derived dendritic cell (BMDCs) stimulated with a viral ligand. In contrast exposure of these cells to SC212 and SC210 resulted in increased IL-10, IL-1β, IL-23, and decreased IL-12p40 following activation of the cells with the inflammatory stimulus LPS. Interestingly SC209 and SC229 had little or no effect on cytokine secretion by BMDCs. Overall, our data demonstrates that these novel fermentates have specific effects and can differentially enhance key immune mechanisms that are critical to viral immune responses, or can suppress responses involved in chronic inflammatory conditions, such as Ulcerative Colitis (UC), and Crohn’s disease (CD).

Keywords

Fermentates; functional food; immune boosting; immunomodulation; anti-inflammatory; viral immunity; chronic inflammation

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.