Yin, H.; Xu, M.; Huang, Q.; Cao, H.; Xie, L.; Yang, F.; Zhang, C.; Sha, G.; Liu, Y. Soil Extracellular Enzyme Activities and Microbial Nutrient Limitation during the Primary Succession of Lava Platform in Wudalianchi, China. Preprints2024, 2024040447. https://doi.org/10.20944/preprints202404.0447.v1
APA Style
Yin, H., Xu, M., Huang, Q., Cao, H., Xie, L., Yang, F., Zhang, C., Sha, G., & Liu, Y. (2024). Soil Extracellular Enzyme Activities and Microbial Nutrient Limitation during the Primary Succession of Lava Platform in Wudalianchi, China. Preprints. https://doi.org/10.20944/preprints202404.0447.v1
Chicago/Turabian Style
Yin, H., Gang Sha and Yingnan Liu. 2024 "Soil Extracellular Enzyme Activities and Microbial Nutrient Limitation during the Primary Succession of Lava Platform in Wudalianchi, China" Preprints. https://doi.org/10.20944/preprints202404.0447.v1
Abstract
Soil extracellular enzyme activity (EEAs) and enzymatic stoichiometry (ES) can provide a crucial indication of changes in soil ecosystem's nutrient availability and the microbial resource limitations. However, the changing characteristics of soil EEAs and ES at different stages of the native succession process and their key drivers are unclear. In order to investigate the soil EEAs, ES and driving factors of soil under vegetation at different succession stages, we adopted the "spatio-temporal substitution" method to collect the surface soil of bryophyte community, herbaceous community, shrub community and tree community in the new volcanic lava platform of Wudalianchi Volcanic Nature Reserve. We measured seven soil EEA, including carbon(C)-acquiring enzyme (β-1,4-glucosidase (BG)), N-acquiring enzymes (β-N-acetyl-glucosaminidase (NAG) and leucine aminopeptidase (LAP)) and phosphorus (P)-acquiring enzyme (acid phosphatase (AP)) activities. The length and angle of vectors defined by ratios of enzyme activities (BG/(NAG + LAP) vs. BG/AP) were used to indicate relative microbial investments in C- (length), and N- and P- (angle) acquiring enzymes. Our results showed that the contents of TC, TN, TP, MBC, DOC and NO3-N in shrub community soil were significantly higher than those in bryophyte, herb and tree communities, and increased by 441%, 246%, 137%, 5570%, 12% and 484%, respectively. The highest soil EEA of C-, N- and P-acquiring were found in shrub community, and the soil EEAs/MBC of C-, N- and P-acquiring were the highest in bryophyte community. Enzyme C:N, C:P and N:P ratios increased progressively in the order of bryophyte, herb and shrub community, but the enzyme C:N, C:P and N:P ratios of tree community were both far less than shrub community. Vector lengths increased progressively in the order of bryophyte (1.16), herb (1.27), tree (1.29) and shrub (1.40), and Vector angles decreased progressively in the order of bryophyte (49.15°), herb (45.65°), Tree (45.31°) and shurb (44.54°), suggested that as succession progresses, soil microbial nutrients transforms from P limitation (angle＞45°) to N limitation (angle＜45°). Redundancy analysis showed that TC, TN, EC and C:N were important drivers of variation in soil EEAs and ES in vegetation at different succession stages. Our findings highlight that the primary succession process cause nutrient limitation transformation. Soil ES might be a sensitive indicator mediated by soil microorganisms to the relative resource limitation at different stages of the primary succession process.
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