Dysregulation of Glypicans and Notum in Osteoarthritis: Plasma Levels, Bone Marrow Mesenchymal Stromal Cells and Osteoblasts.

IRENE GONZALEZ-GUEDE; Maria Lopez-Ramos; Luis Rodríguez-Rodriguez; Lydia Abasolo; Arkaitz Mucientes; Benjamín Fernández-Gutiérrez

doi:10.20944/preprints202404.0001.v1

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preprints.org > medicine and pharmacology > clinical medicine > doi: 10.20944/preprints202404.0001.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Dysregulation of Glypicans and Notum in Osteoarthritis: Plasma Levels, Bone Marrow Mesenchymal Stromal Cells and Osteoblasts.

IRENE GONZALEZ-GUEDE

Maria Lopez-Ramos

Luis Rodríguez-Rodriguez

Lydia Abasolo

Arkaitz Mucientes

Benjamín Fernández-Gutiérrez

Version 1 : Received: 26 March 2024 / Approved: 29 March 2024 / Online: 1 April 2024 (09:47:08 CEST)

How to cite: GONZALEZ-GUEDE, I.; Lopez-Ramos, M.; Rodríguez-Rodriguez, L.; Abasolo, L.; Mucientes, A.; Fernández-Gutiérrez, B. Dysregulation of Glypicans and Notum in Osteoarthritis: Plasma Levels, Bone Marrow Mesenchymal Stromal Cells and Osteoblasts.. Preprints 2024, 2024040001. https://doi.org/10.20944/preprints202404.0001.v1 GONZALEZ-GUEDE, I.; Lopez-Ramos, M.; Rodríguez-Rodriguez, L.; Abasolo, L.; Mucientes, A.; Fernández-Gutiérrez, B. Dysregulation of Glypicans and Notum in Osteoarthritis: Plasma Levels, Bone Marrow Mesenchymal Stromal Cells and Osteoblasts.. Preprints 2024, 2024040001. https://doi.org/10.20944/preprints202404.0001.v1

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MDPI and ACS Style

GONZALEZ-GUEDE, I.; Lopez-Ramos, M.; Rodríguez-Rodriguez, L.; Abasolo, L.; Mucientes, A.; Fernández-Gutiérrez, B. Dysregulation of Glypicans and Notum in Osteoarthritis: Plasma Levels, Bone Marrow Mesenchymal Stromal Cells and Osteoblasts.. Preprints 2024, 2024040001. https://doi.org/10.20944/preprints202404.0001.v1

APA Style

GONZALEZ-GUEDE, I., Lopez-Ramos, M., Rodríguez-Rodriguez, L., Abasolo, L., Mucientes, A., & Fernández-Gutiérrez, B. (2024). Dysregulation of Glypicans and Notum in Osteoarthritis: Plasma Levels, Bone Marrow Mesenchymal Stromal Cells and Osteoblasts.. Preprints. https://doi.org/10.20944/preprints202404.0001.v1

Chicago/Turabian Style

GONZALEZ-GUEDE, I., Arkaitz Mucientes and Benjamín Fernández-Gutiérrez. 2024 "Dysregulation of Glypicans and Notum in Osteoarthritis: Plasma Levels, Bone Marrow Mesenchymal Stromal Cells and Osteoblasts." Preprints. https://doi.org/10.20944/preprints202404.0001.v1

Abstract

Study of alterations in Osteoarthritis (OA) of Glypicans 1 to 6 (GPCs) and Notum in plasma, bone marrow mesenchymal stromal cells (BM-MSCs) and osteoblasts. Plasma levels of GPCs and Notum were measured of 25 patients and 24 healthy subjects. In addition, BM-MSCs from eight OA patients and eight healthy donors were cultured during 21 days with culture me-dium and osteogenic medium. Protein and gene expression levels of GPCs and Notum were de-termined by ELISA and qPCR at 0, 7 ,14 and 21 days. GPC5 and Notum have been found de-creased in OA patients’ plasma. OA BM-MSCs showed downexpression of GPC6 and upregula-tion of Notum. A decrease in GPC5 and Notum proteins and an increase in GPC3 were found. During osteogenic differentiation, elevated GPC2, 4, 5, 6 and Notum mRNA levels and decreased GPC3 were observed in OA. Furthermore, the protein levels of GPC2, GPC5 and Notum de-creased and GPC3 increased. Glypicans and Notum are altered in BM-MSCs and during osteo-genic differentiation from patients with OA. Furthermore, GPC5 and Notum can be proposed as new biomarkers of the pathology. The alterations found indicate that Notum could be a prom-ising therapeutic strategy for Osteoarthritis.

Keywords

Osteoarthritis; Bone Marrow Mesenchymal Stem Cells; Osteoblast; Glypican; NOTUM

Subject

Medicine and Pharmacology, Clinical Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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