Version 1
: Received: 14 March 2024 / Approved: 15 March 2024 / Online: 15 March 2024 (08:54:24 CET)
How to cite:
Bošnir, J.; Bevardi, M.; Hećimović, I.; Budeč, M.; Juranović Cindrić, I.; Kober, R.; Jurak, G.; Lasić, D.; Racz, A. Optimization of Sample Preparation Procedure for Determina-Tion of Fat-Soluble Vitamins in Milk and Infant Food by HPLC Technique. Preprints2024, 2024030889. https://doi.org/10.20944/preprints202403.0889.v1
Bošnir, J.; Bevardi, M.; Hećimović, I.; Budeč, M.; Juranović Cindrić, I.; Kober, R.; Jurak, G.; Lasić, D.; Racz, A. Optimization of Sample Preparation Procedure for Determina-Tion of Fat-Soluble Vitamins in Milk and Infant Food by HPLC Technique. Preprints 2024, 2024030889. https://doi.org/10.20944/preprints202403.0889.v1
Bošnir, J.; Bevardi, M.; Hećimović, I.; Budeč, M.; Juranović Cindrić, I.; Kober, R.; Jurak, G.; Lasić, D.; Racz, A. Optimization of Sample Preparation Procedure for Determina-Tion of Fat-Soluble Vitamins in Milk and Infant Food by HPLC Technique. Preprints2024, 2024030889. https://doi.org/10.20944/preprints202403.0889.v1
APA Style
Bošnir, J., Bevardi, M., Hećimović, I., Budeč, M., Juranović Cindrić, I., Kober, R., Jurak, G., Lasić, D., & Racz, A. (2024). Optimization of Sample Preparation Procedure for Determina-Tion of Fat-Soluble Vitamins in Milk and Infant Food by HPLC Technique. Preprints. https://doi.org/10.20944/preprints202403.0889.v1
Chicago/Turabian Style
Bošnir, J., Dario Lasić and Aleksandar Racz. 2024 "Optimization of Sample Preparation Procedure for Determina-Tion of Fat-Soluble Vitamins in Milk and Infant Food by HPLC Technique" Preprints. https://doi.org/10.20944/preprints202403.0889.v1
Abstract
Abstract: Background: The analysis of vitamins in baby food is a challenging task given the com-plexity of the food matrix, vitamin stability, and strict regulations of the European Union regarding permissible deviations from declared values. Vitamins in food exist in different concentrations and forms and have different stabilities, and it is not straightforward to prepare samples for reliable analysis using the same procedure. Therefore, significant attention has been devoted to optimizing sample preparation in the analysis of vitamins. Methods: This study aims to determine which of the seven applied extraction methods is the most efficient for the simultaneous extraction and deter-mination of A, D, E, and K vitamins in milk and baby food using high-performance liquid chro-matography (HPLC). Different samples of baby food were prepared in seven different ways based on four methods (saponification, enzymatic hydrolysis, solvent extraction, and solid-phase extrac-tion). Results and Conclusion: The best preparation method proved to be solid-phase extraction with a C18 stationary phase, and HPLC with a UV-VIS detector was identified as a sufficiently sensitive technique for the identification and quantification of fat-soluble vitamins in milk and baby food.
Keywords
Vitamins; baby food; extraction; HPLC
Subject
Chemistry and Materials Science, Food Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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