This version is not peer-reviewed.
Submitted:
09 March 2024
Posted:
12 March 2024
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S.NO. | Groups | Protein Concentration in a Sample (mg/mL) |
---|---|---|
1- | Total Pooled Venom | 110 |
2- | Crude Venom (Diluted 10x) | 9.8 |
3- | Fraction-1 | 90.3 |
4- | Fraction-2 | 0.81 |
5- | Standard (BSA) | 2 |
Groups | Dose (µg/Kg) | Dose difference (a) | No. of animals (n=8) | No. of Death | Mean Mortality (b) | Product (a X b) |
---|---|---|---|---|---|---|
1. | 27 | - | 8 | 1 | - | - |
2. | 30 | 3 | 8 | 2 | 1.5 | 4.5 |
3. | 33 | 3 | 8 | 3 | 2.5 | 7.5 |
4. | 40 | 7 | 8 | 8 | 5.5 | 38.5 |
5. | 47 | 7 | 8 | 8 | 8 | 56 |
6. | 50 | 3 | 8 | 8 | 8 | 24 |
7. | 53 | 3 | 8 | 8 | 8 | 24 |
∑= 154.5 |
Groups | Dose (µg/Kg) | Dose difference (a) | No. of animals (n=8) | No. of Death | Mean Mortality (b) | Product (a X b) |
---|---|---|---|---|---|---|
1. | 5 | - | 8 | 2 | - | - |
2. | 6 | 1 | 8 | 3 | 2.5 | 2.5 |
3. | 7.3 | 1.3 | 8 | 4 | 3.5 | 4.6 |
4. | 8.3 | 1 | 8 | 6 | 5 | 5 |
5. | 13.2 | 4.9 | 8 | 8 | 7 | 34.3 |
6. | 15 | 1.8 | 8 | 8 | 8 | 14.4 |
7. | 17 | 2 | 8 | 8 | 8 | 16 |
8. | 21 | 4 | 8 | 8 | 8 | 32 |
9. | 30 | 9 | 8 | 8 | 8 | 72 |
∑= 180.8 |
Groups | Dose (µg/Kg) | Dose difference (a) | No. of animals (n=8) | No. of Death | Mean Mortality (b) | Product (a X b) |
---|---|---|---|---|---|---|
1. | 333 | - | 8 | 4 | - | - |
2. | 500 | 167 | 8 | 6 | 5 | 835 |
3. | 667 | 167 | 8 | 8 | 7 | 1169 |
4. | 1000 | 333 | 8 | 8 | 8 | 2664 |
5. | 1333 | 333 | 8 | 8 | 8 | 2664 |
6. | 1667 | 334 | 8 | 8 | 8 | 2672 |
7. | 2000 | 333 | 8 | 8 | 8 | 2664 |
8. | 2666 | 666 | 8 | 8 | 8 | 5328 |
∑= 17996 |
S.No# | Groups | Body weight (gm.) (n=5) | Dose (ug/kg) | Mortality (n = 5) |
---|---|---|---|---|
1. | Crude Venom | 23.5 ± 0.5 | 27 | 1/5 |
24.3 ± 0.5 | 25.5 | 0/5 | ||
25.5 ± 0.4 | 19 | 0/5 | ||
2. | Fraction-1 | 22 ± 0.6 | 9.5 | 0/5 |
25.7 ± 0.6 | 8.3 | 3/5 | ||
26.6 ± 0.7 | 6 | 2/5 | ||
28.4 ± 0.3 | 5 | 1/5 | ||
27.6 ± 0.2 | 3.7 | 0/5 | ||
3. | Fraction-2 | 24.5 ± 0.6 | 500 | 3/5 |
22.5 ± 0.5 | 333 | 1/5 | ||
24.5 ± 0.5 | 267 | 0/5 | ||
25.2 ± 0.5 | 200 | 0/5 | ||
4. | Control | 25 ± 0.6 | 0.1mL N/S | 0/5 |
CBC Profile | |||||
---|---|---|---|---|---|
S.NO | Groups | Hb (g/dl) | RBC (10e12/L) | WBC (10e9/L) | PLT (10e9L) |
1. | Crude (27 µg/kg) | 13.2 ± 0.38*** | 8.2 ± 0.35** | 3.2 ± 0.38ns | 486.3 ± 3.17ns |
2. | Fraction-1 (5 µg/kg) | 21.1 ± 0.43*** | 13.3 ± 0.32*** | 15.1 ± 0.17*** | 600 ± 3.46** |
3. | Fraction-2 (333 µg/kg) | 13.1 ± 0.31*** | 8.7 ± 0.17** | 11.6 ± 0.34*** | 654.7 ± 22.6*** |
4. | Control | 7.8 ± 0.37 | 5.2 ± 0.63 | 3.7 ± 0.32 | 507 ± 6.35 |
Biochemical Parameters | |||||
---|---|---|---|---|---|
S.NO | Groups | AST (U/L) | ALT (U/L) | Cr. (mg/dl/l) | BUN (mg/dl) |
1. | Crude (27ug/kg) | 640.7 ± 2.6*** | 46 ± 1.7ns | 0.45 ± 0.03ns | 9.4 ± 0.17ns |
2. | Fraction-1 (5ug/kg) | 762.7 ± 2.3*** | 83 ± 1.1*** | 0.51 ± 0.03* | 15.4 ± 0.32*** |
3. | Fraction-2 (333ug/kg) | 462 ± 3.9*** | 68.7 ± 1.4*** | 0.47 ± 0.02ns | 14.4 ± 0.12*** |
4. | Control | 387.6 ± 7.5 | 52.7 ± 2.6 | 0.38 ± 0.01 | 9.1 ± 0.11 |
S.No# | Groups | Body weight (gm.) | Dose (ug/kg) | Mortality (n = 5) |
---|---|---|---|---|
1. | Crude Venom | 24.3 ± 0.5 | 25.5 | 3/5 |
25.5 ± 0.6 | 19 | 0/5 | ||
22 ± 0.5 | 9.5 | 0/5 | ||
2. | Fraction-1 | 28.4 ± 0.4 | 5 | 4/5 |
27.6 ± 0.3 | 3.7 | 3/5 | ||
26.9 ± 0.2 | 1.8 | 0/5 | ||
3. | Fraction-2 | 22.5 ± 0.9 | 333 | 3/5 |
24.5 ± 0.5 | 267 | 0/5 | ||
25.2 ± 0.6 | 200 | 0/5 | ||
4. | Control | 25 ± 0.7 | 0.1 mL N/S | 0/5 |
CBC Profile | |||||
---|---|---|---|---|---|
S.NO | Groups | Hb (g/dl) | RBC (10e12/L) | WBC (10e9/L) | PLT (10e9/L) |
1. | Crude (19 µg/kg) | 12.8 ± 0.20** | 8.6 ± 0.51* | 13.7 ± 0.26*** | 654.7 ±33* |
2. | Fraction-1 (1.8 µg/kg) | 22.8 ± 0.14*** | 17 ± 0.17*** | 16.3 ± 0.26*** | 715 ± 39 ** |
3. | Fraction-2 (267 µg/kg) | 8.8 ± 2.0ns | 6.6 ± 0.76ns | 11.9 ± 0.29*** | 793 ± 9.41*** |
4. | Control | 8.5 ± 0.17 | 5.87 ± 0.52 | 4.6 ± 0.56 | 487.7 ± 41 |
Biochemical Parameters | |||||
---|---|---|---|---|---|
S.NO | Groups | AST (U/L) | ALT (U/L) | Creatinine (mg/dl/l) | BUN (mg/dl) |
1. | Crude (19ug/kg) | 749 ± 10.6*** | 84 ± 2.11*** | 0.38 ± 0.01ns | 8.3 ± 0.11*** |
2. | Fraction-1 (1.8ug/kg) | 277.3 ± 5.4*** | 56.3 ± 0.90ns | 0.44 ± 0.01ns | 10.5 ± 0.11** |
3. | Fraction-2 (267ug/kg) | 642.6 ± 4.6*** | 92 ± 1.73*** | 0.41 ± 0.01ns | 8.43 ± 0.09*** |
4. | Control | 394.3 ± 4.8 | 55.7 ± 2.40 | 0.41 ± 0.01 | 9.7 ± 0.18 |
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