Cho, H.; Hong, N.-K.; Chang, Y.-T. Novel Fluorescent Strategy for Discriminating T and B Lymphocytes Using Transport System. Pharmaceutics2024, 16, 424.
Cho, H.; Hong, N.-K.; Chang, Y.-T. Novel Fluorescent Strategy for Discriminating T and B Lymphocytes Using Transport System. Pharmaceutics 2024, 16, 424.
Cho, H.; Hong, N.-K.; Chang, Y.-T. Novel Fluorescent Strategy for Discriminating T and B Lymphocytes Using Transport System. Pharmaceutics2024, 16, 424.
Cho, H.; Hong, N.-K.; Chang, Y.-T. Novel Fluorescent Strategy for Discriminating T and B Lymphocytes Using Transport System. Pharmaceutics 2024, 16, 424.
Abstract
Fluorescent bioprobes are invaluable tools for visualizing live cells and deciphering complex biological processes by targeting intracellular biomarkers without disrupting cellular functions. In addition to protein-binding concepts, fluorescent probes utilize various mechanisms, including membrane, metabolism, and gating-oriented strategies. This study introduces a novel fluorescent mechanism distinct from existing ways. Here, we developed a B-cell selective probe, CDrB, with unique transport mechanisms. Through SLC-CRISPRa screening, we identified two transporters, SLCO1B3 and SLC25A41, by sorting out populations exhibiting higher and lower fluorescence intensities, respectively, demonstrating contrasting activities. We confirmed that SLCO1B3, with comparable expression levels in T and B cells, facilitates the transport of CDrB into cells, while SLC25A41, overexpressed in T lymphocytes, actively exports CDrB. The observation suggests that SLC25A41 plays a crucial role in discriminating between T and B lymphocytes. Furthermore, it reveals the potential for reversible localization of SLC25A41 to demonstrate its distinct activity. This study is the first report to unveil a novel strategy of SLC by exporting the probe. We anticipate that this research will open up new avenues for developing fluorescent probes.
Keywords
Fluorescent carbohydrate library; High-throughput phenotypic screening; Gating-Oriented Live-cell Distinction; Solute carrier; B cell specific probe
Subject
Chemistry and Materials Science, Other
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
