Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Comparative Targeted Metabolomics in Ischemic Stroke Patients: Profiling Thrombi and Serums Samples for Identification of Stroke-related Metabolites

Ivana Karmelić
* ORCID logo ,
Ivana Rubić
,
Katarina Starčević
,
David Ozretić
ORCID logo ,
Zdravka Poljaković
,
Mia Jurilj Sajko
,
Vladimir Kalousek
ORCID logo ,
Rafaela Kalanj
,
Dina Rešetar-Maslov
,
Josipa Kuleš
,
Marina Roje Bedeković
,
Tomislav Sajko
,
Krešimir Rotim
,
Vladimir Mrljak
,
Dragana Fabris
* ORCID logo
Version 1 : Received: 21 February 2024 / Approved: 21 February 2024 / Online: 21 February 2024 (11:16:54 CET)

How to cite: Karmelić, I.; Rubić, I.; Starčević, K.; Ozretić, D.; Poljaković, Z.; Sajko, M.J.; Kalousek, V.; Kalanj, R.; Rešetar-Maslov, D.; Kuleš, J.; Roje Bedeković, M.; Sajko, T.; Rotim, K.; Mrljak, V.; Fabris, D. Comparative Targeted Metabolomics in Ischemic Stroke Patients: Profiling Thrombi and Serums Samples for Identification of Stroke-related Metabolites. Preprints 2024, 2024021223. https://doi.org/10.20944/preprints202402.1223.v1 Karmelić, I.; Rubić, I.; Starčević, K.; Ozretić, D.; Poljaković, Z.; Sajko, M.J.; Kalousek, V.; Kalanj, R.; Rešetar-Maslov, D.; Kuleš, J.; Roje Bedeković, M.; Sajko, T.; Rotim, K.; Mrljak, V.; Fabris, D. Comparative Targeted Metabolomics in Ischemic Stroke Patients: Profiling Thrombi and Serums Samples for Identification of Stroke-related Metabolites. Preprints 2024, 2024021223. https://doi.org/10.20944/preprints202402.1223.v1

Abstract

Ischemic stroke is one of the leading causes of death and permanent disability in the world. Rapid diagnosis and intervention are crucial for reducing its consequences on individuals and societies. Identifying reliable biomarkers for early detection, prognostics and therapy is a criti-cal imperative for improving the stroke patient treatment. Metabolomics has been shown as a promising tool for biomarker discovery since many of the post-ischemic metabolites can found in the plasma or serum of the patient. In this research, we have performed comparative targeted metabolomic analysis of stroke thrombi, patient serums and healthy control serums in order to determine the alteration in the patients’ metabolome. The most statistically altered metabolites characterized in patient compared to control serums were glutamate and serotonin, followed by several lipid metabolites (phospholipids and triacylglycerols) and glucose. In stroke thrombi compared to patient’s serums, the most significantly altered metabolites were classified as li-pids, with choline-containing phospholipids and sphingomyelins having the highest discrimi-natory score. The results of this study could help in understanding the roles of different meta-bolic changes occurring during thrombosis and cerebral ischemia and possibly suggest new metabolic biomarkers of the ischemic stroke.

Keywords

ischemic stroke; stroke thrombi; targeted metabolomics; metabolic biomarkers

Subject

Medicine and Pharmacology, Clinical Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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