Wójciuk, K.E.; Sadło, J.; Lewandowska, H.; Brzóska, K.; Kruszewski, M. A Crucial Role of Proteolysis in the Formation of Intracellular Dinitrosyl Iron Complexes. Molecules2024, 29, 1630.
Wójciuk, K.E.; Sadło, J.; Lewandowska, H.; Brzóska, K.; Kruszewski, M. A Crucial Role of Proteolysis in the Formation of Intracellular Dinitrosyl Iron Complexes. Molecules 2024, 29, 1630.
Wójciuk, K.E.; Sadło, J.; Lewandowska, H.; Brzóska, K.; Kruszewski, M. A Crucial Role of Proteolysis in the Formation of Intracellular Dinitrosyl Iron Complexes. Molecules2024, 29, 1630.
Wójciuk, K.E.; Sadło, J.; Lewandowska, H.; Brzóska, K.; Kruszewski, M. A Crucial Role of Proteolysis in the Formation of Intracellular Dinitrosyl Iron Complexes. Molecules 2024, 29, 1630.
Abstract
Pretreatment of cells with proteolysis inhibitors diminished intensity and changed shape of DNICs specific EPR signal in a treatment time-dependent manner. The level of DNIC formation was significantly influenced by the presence of protein degradation products. Interestingly, formation of HMWDNICs depended on the availability of LMWDNICs. The extent of glutathione involvement in the in vivo formation of DNICs is minor yet noticeable, aligning with our prior research findings.
Keywords
K562 cells; electron paramagnetic resonance; nitric oxide; reactive nitrogen species; dinitrosyl iron complexes; chelatable iron; glutathione
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
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