Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Surface Topography, Microbial Adhesion, and Immune Responses in Silicone Mammary Implant-Associated Capsular Fibrosis

Ines Schoberleitner
ORCID logo ,
Leoni Baier
,
Michaela Lackner
ORCID logo ,
Lisa-Maria Zenz
,
Debora C. Coraca-Huber
ORCID logo ,
Wendy Ullmer
,
Annabelle Damerun
,
Klaus Faserl
ORCID logo ,
Stephan Sigl
,
Theresia Steinkellner
,
Selina Winkelmann
,
Bettina Sarg
,
Daniel Egle
,
Christine Brunner
,
Dolores Wolfram
* ORCID logo
Version 1 : Received: 30 January 2024 / Approved: 31 January 2024 / Online: 31 January 2024 (05:46:56 CET)

A peer-reviewed article of this Preprint also exists.

Schoberleitner, I.; Baier, L.; Lackner, M.; Zenz, L.-M.; Coraça-Huber, D.C.; Ullmer, W.; Damerum, A.; Faserl, K.; Sigl, S.; Steinkellner, T.; Winkelmann, S.; Sarg, B.; Egle, D.; Brunner, C.; Wolfram, D. Surface Topography, Microbial Adhesion, and Immune Responses in Silicone Mammary Implant-Associated Capsular Fibrosis. Int. J. Mol. Sci. 2024, 25, 3163. Schoberleitner, I.; Baier, L.; Lackner, M.; Zenz, L.-M.; Coraça-Huber, D.C.; Ullmer, W.; Damerum, A.; Faserl, K.; Sigl, S.; Steinkellner, T.; Winkelmann, S.; Sarg, B.; Egle, D.; Brunner, C.; Wolfram, D. Surface Topography, Microbial Adhesion, and Immune Responses in Silicone Mammary Implant-Associated Capsular Fibrosis. Int. J. Mol. Sci. 2024, 25, 3163.

Abstract

Breast cancer is the most common cancer in women globally, often necessitating mastectomy and subsequent breast reconstruction. Silicone mammary implants (SMIs) play a pivotal role in breast reconstruction, yet their interaction with the host immune system and microbiome remains poorly understood. This study investigates the impact of SMI surface topography on host antimicrobial responses, wound proteome dynamics, and microbial colonization. Biological samples were col-lected from ten human patients undergoing breast reconstruction with SMIs. Mass spectrometry profiles were analyzed for acute and chronic wound proteomes, revealing a nuanced interplay between topography and antimicrobial respondseproteins. 16S rRNA sequencing assessed mi-crobiome dynamics, unveiling topography-specific variations in microbial composition. Surface topography alterations influenced wound proteome composition. Microbiome analysis revealed heightened diversity around rougher SMIs, emphasizing topography-dependent microbial inva-sion. In vitro experiments confirmed staphylococcal adhesion, growth, and biofilm formation on SMI surfaces, with increased texture correlating positively with bacterial colonization. This comprehensive investigation highlights the intricate interplay between SMI topography, wound proteome dynamics, and microbial transmission. The findings contribute to understanding host-microbe interactions on SMI surfaces, essential for optimizing clinical applications and minimizing complications in breast reconstruction.

Keywords

implant-based breast reconstruction; SMI surface roughness; foreign body response (FBR); acute wound proteome; chronic wound proteome; antimicrobial humoral response; capsular fibrosis; surgical site microbiome; SMI-adhesive microbiome; implant-associated biofilm formation; SMI topography specific antimicrobial response; Staphylococcus transmission at surgical site; immunomics

Subject

Medicine and Pharmacology, Immunology and Allergy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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