Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Negevirus Piura Suppresses Zika Virus Replication in Mosquito Cells

Valéria Lima Carvalho
* ORCID logo ,
Dhani Prakoso
ORCID logo ,
Erika R. Schwarz
,
Tracey D. Logan
ORCID logo ,
Bruno Tardelli Diniz Nunes
ORCID logo ,
Sarah E. Beachboard
,
Maureen T. Long
* ORCID logo
Version 1 : Received: 30 January 2024 / Approved: 31 January 2024 / Online: 31 January 2024 (06:00:26 CET)

A peer-reviewed article of this Preprint also exists.

Carvalho, V.L.; Prakoso, D.; Schwarz, E.R.; Logan, T.D.; Nunes, B.T.D.; Beachboard, S.E.; Long, M.T. Negevirus Piura Suppresses Zika Virus Replication in Mosquito Cells. Viruses 2024, 16, 350. Carvalho, V.L.; Prakoso, D.; Schwarz, E.R.; Logan, T.D.; Nunes, B.T.D.; Beachboard, S.E.; Long, M.T. Negevirus Piura Suppresses Zika Virus Replication in Mosquito Cells. Viruses 2024, 16, 350.

Abstract

We investigated the interaction between the insect-specific virus, Piura virus (PIUV) and the arbovirus Zika virus (ZIKV) in Aedes albopictus cells. We performed coinfection experiments in C6/36 cells. Piura virus (Cor 33 strain, Colombia) and ZIKV (PRVABC58 strain, Puerto Rico) were co-inoculated into C6/36 cells using two MOI combinations: 0.1 for both viruses and 1.0 for ZIKV, 0.1 for PIUV. Wells were infected in triplicate with either PIUV and ZIKV coinfection, ZIKV-only, or PIUV-only. Mock infected cells served as control wells. The cell suspension was collected daily through 7 days post-infection. Zika virus load was titrated by TCID50 on Vero 76 cells. The ZIKV-only infection and PIUV and ZIKV coinfection experiments were also quantified by RT-qPCR. We also investigated whether ZIKV interfered in the PIUV replication. PIUV suppressed the replication of ZIKV resulting in a 10,000-fold reduction in ZIKV titers within 3 days post-infection. PIUV viral loads were not reduced in the presence of ZIKV. We conclude that, when concurrently infected, PIUV suppresses ZIKV in C6/36 cells while ZIKV does not interfere in PIUV replication.

Keywords

Zika virus; Piura virus; coinfection

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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