Equine Metacarpophalangeal Joint Partial and Full Thickness Defects Treated with Allogenic Equine Synovial Membrane Mesenchymal Stem/Stromal Cell and Umbilical Cord Mesenchymal Stem/Stromal Cell Conditioned Medium: The Proof-of-Concept

I.L. Reis; B. Lopes; P. Sousa; A.C. Sousa; Ana Rita Caseiro; I. Briote; Ana Matilde Rocha; J.P. Pereira; C.M. Mendonça; J.M. Santos; Luís Lamas; L.M. Atayde; R.D. Alvites; Ana Colette Maurício

doi:10.20944/preprints202401.1915.v1

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preprints.org > medicine and pharmacology > veterinary medicine > doi: 10.20944/preprints202401.1915.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Equine Metacarpophalangeal Joint Partial and Full Thickness Defects Treated with Allogenic Equine Synovial Membrane Mesenchymal Stem/Stromal Cell and Umbilical Cord Mesenchymal Stem/Stromal Cell Conditioned Medium: The Proof-of-Concept

Version 1 : Received: 25 January 2024 / Approved: 26 January 2024 / Online: 26 January 2024 (13:56:06 CET)

How to cite: Reis, I.; Lopes, B.; Sousa, P.; Sousa, A.; Caseiro, A.R.; Briote, I.; Rocha, A.M.; Pereira, J.; Mendonça, C.; Santos, J.; Lamas, L.; Atayde, L.; Alvites, R.; Maurício, A.C. Equine Metacarpophalangeal Joint Partial and Full Thickness Defects Treated with Allogenic Equine Synovial Membrane Mesenchymal Stem/Stromal Cell and Umbilical Cord Mesenchymal Stem/Stromal Cell Conditioned Medium: The Proof-of-Concept. Preprints 2024, 2024011915. https://doi.org/10.20944/preprints202401.1915.v1 Reis, I.; Lopes, B.; Sousa, P.; Sousa, A.; Caseiro, A.R.; Briote, I.; Rocha, A.M.; Pereira, J.; Mendonça, C.; Santos, J.; Lamas, L.; Atayde, L.; Alvites, R.; Maurício, A.C. Equine Metacarpophalangeal Joint Partial and Full Thickness Defects Treated with Allogenic Equine Synovial Membrane Mesenchymal Stem/Stromal Cell and Umbilical Cord Mesenchymal Stem/Stromal Cell Conditioned Medium: The Proof-of-Concept. Preprints 2024, 2024011915. https://doi.org/10.20944/preprints202401.1915.v1

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MDPI and ACS Style

Reis, I.; Lopes, B.; Sousa, P.; Sousa, A.; Caseiro, A.R.; Briote, I.; Rocha, A.M.; Pereira, J.; Mendonça, C.; Santos, J.; Lamas, L.; Atayde, L.; Alvites, R.; Maurício, A.C. Equine Metacarpophalangeal Joint Partial and Full Thickness Defects Treated with Allogenic Equine Synovial Membrane Mesenchymal Stem/Stromal Cell and Umbilical Cord Mesenchymal Stem/Stromal Cell Conditioned Medium: The Proof-of-Concept. Preprints 2024, 2024011915. https://doi.org/10.20944/preprints202401.1915.v1

APA Style

Reis, I., Lopes, B., Sousa, P., Sousa, A., Caseiro, A.R., Briote, I., Rocha, A.M., Pereira, J., Mendonça, C., Santos, J., Lamas, L., Atayde, L., Alvites, R., & Maurício, A.C. (2024). Equine Metacarpophalangeal Joint Partial and Full Thickness Defects Treated with Allogenic Equine Synovial Membrane Mesenchymal Stem/Stromal Cell and Umbilical Cord Mesenchymal Stem/Stromal Cell Conditioned Medium: The Proof-of-Concept. Preprints. https://doi.org/10.20944/preprints202401.1915.v1

Chicago/Turabian Style

Reis, I., R.D. Alvites and Ana Colette Maurício. 2024 "Equine Metacarpophalangeal Joint Partial and Full Thickness Defects Treated with Allogenic Equine Synovial Membrane Mesenchymal Stem/Stromal Cell and Umbilical Cord Mesenchymal Stem/Stromal Cell Conditioned Medium: The Proof-of-Concept" Preprints. https://doi.org/10.20944/preprints202401.1915.v1

Abstract

Articular cartilage healing and repair is one of the biggest clinical challenges in trauma and orthopaedic medicine, leading to high economic loss due to athletic impairment in sport horses. Multipotent Mesenchymal stem/stromal cells are part of novel therapeutic arsenal aimed at tissue regeneration and restoring organ function. These cells release biomolecules and factors in the culture media, producing the so called conditioned medium, which have a pro-regenerative effect through paracrine mechanisms. Considering this, a therapeutic product combining equine synovial membrane derived Mesenchymal stem/stromal cells and equine umbilical cord stroma-derived Mesenchymal stem/stromal cells conditioned medium was employed. In this work the treatment of two articular defects, in the first phalanx and third metacarpus of the left front limb of a five-year-old horse, is described. Following surgical debridement of the lesions, the treatment comprised two intra-articular administrations of the above-mentioned therapeutic combination, 15 days apart. This was followed by a thorough rehabilitation program. A second-round treatment was performed 6 months later. Diagnostic imaging demonstrated significant improvement of the articular defects. The clinical outcome allowed a full return to the athletic performance demonstrating the safety and potential advantages of this approach when compared with other therapeutic options.

Keywords

Equine; mesenchymal stem/stromal cells; synovial membrane mesenchymal stem cell; umbilical cord conditioned medium; clinical case; cartilage; subchondral bone; osteoarthritis; allogeneic cell-based therapies; horses.

Subject

Medicine and Pharmacology, Veterinary Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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