Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Downregulation of TASK-3 Channel Induces Senescence of Granulosa Cells in the Bovine Follicular Cystic Ovary

Chang-Woon Kim
,
Eun-Jin Kim
,
Min Seok Woo
ORCID logo ,
Dang Long Cao
,
Asifiwe Clarisse Cirunduzi
,
Ji Hyeon Ryu
,
Il-Keun Kong
,
Dong Kun Lee
ORCID logo ,
Seong-Geun Hong
,
Jaehee Han
,
Dawon Kang
* ORCID logo
Version 1 : Received: 20 January 2024 / Approved: 22 January 2024 / Online: 22 January 2024 (05:53:13 CET)

How to cite: Kim, C.; Kim, E.; Woo, M.S.; Cao, D.L.; Cirunduzi, A.C.; Ryu, J.H.; Kong, I.; Lee, D.K.; Hong, S.; Han, J.; Kang, D. Downregulation of TASK-3 Channel Induces Senescence of Granulosa Cells in the Bovine Follicular Cystic Ovary. Preprints 2024, 2024011560. https://doi.org/10.20944/preprints202401.1560.v1 Kim, C.; Kim, E.; Woo, M.S.; Cao, D.L.; Cirunduzi, A.C.; Ryu, J.H.; Kong, I.; Lee, D.K.; Hong, S.; Han, J.; Kang, D. Downregulation of TASK-3 Channel Induces Senescence of Granulosa Cells in the Bovine Follicular Cystic Ovary. Preprints 2024, 2024011560. https://doi.org/10.20944/preprints202401.1560.v1

Abstract

Ovarian cysts are linked to hormone imbalances and altered gene expressions, but the connection between cysts and ion channel expression is understudied. This study explored the role of TWIK-related acid-sensitive K+ (TASK) channels in bovine ovarian cyst formation. The ovarian follicles were split into small (5 to 10 mm in diameter) and large (> 25 mm in diameter) groups. Among the measured K+, Na+, and Cl- concentrations in follicular fluid (FF) obtained from small-sized follicles (SF) and large-sized follicles (LF), the K+ concentration was significantly lower in LFFF. Quantitative PCR, western blot, and immunocytochemistry data revealed that TASK-3 expression levels significantly decreased by approximately 50% in both LF and granulosa cells obtained from LF (LFGCs) compared to the corresponding controls. The TASK-3 protein was localized to the plasma membrane of GCs. The diameter of LFGCs was larger than that of SFGC. The cell swelling response to exposure to a hypotonic solution (200 mOsm/L) was highly reduced in TASK-3-overexpressing cells compared to vector-transfected cells. TASK-3-knockdown cells showed arrested growth. Senescence markers were detected in LFGCs and TASK-3-knockdown cells. These findings suggest that reduced TASK-3 expression in LF is associated with the inhibition of GC growth, leading to senescence and cyst formation.

Keywords

granulosa cell; ovarian cyst; potassium ion; TASK-3 channel

Subject

Medicine and Pharmacology, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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