Barnea, I.; Luria, L.; Girsault, A.; Dabah, O.; Dudaie, M.; Mirsky, S.K.; Merkel, D.; Shaked, N.T. Analyzing Blood Cells of High-Risk Myelodysplastic Syndrome Patients Using Interferometric Phase Microscopy and Fluorescent Flow Cytometry. Bioengineering2024, 11, 256.
Barnea, I.; Luria, L.; Girsault, A.; Dabah, O.; Dudaie, M.; Mirsky, S.K.; Merkel, D.; Shaked, N.T. Analyzing Blood Cells of High-Risk Myelodysplastic Syndrome Patients Using Interferometric Phase Microscopy and Fluorescent Flow Cytometry. Bioengineering 2024, 11, 256.
Barnea, I.; Luria, L.; Girsault, A.; Dabah, O.; Dudaie, M.; Mirsky, S.K.; Merkel, D.; Shaked, N.T. Analyzing Blood Cells of High-Risk Myelodysplastic Syndrome Patients Using Interferometric Phase Microscopy and Fluorescent Flow Cytometry. Bioengineering2024, 11, 256.
Barnea, I.; Luria, L.; Girsault, A.; Dabah, O.; Dudaie, M.; Mirsky, S.K.; Merkel, D.; Shaked, N.T. Analyzing Blood Cells of High-Risk Myelodysplastic Syndrome Patients Using Interferometric Phase Microscopy and Fluorescent Flow Cytometry. Bioengineering 2024, 11, 256.
Abstract
(1) Background: Myelodysplastic syndromes (MDS) are a group of diseases that affect the morphology and function of neutrophils. Rapid diagnosis of MDS is crucial for the initiation of treatment that can vastly improve disease outcome. (2) methods: In this work, we present a new approach for detecting morphological differences between neutrophils isolated from blood samples of high risk MDS patients and that for patients and blood bank donors (BBDs). First, using fluorescent flow cytometry CD66b positive cells were stained with 2′,7′-dichlorofluorescin diacetate (DCF) and Hoechst. Secondly, we imaged the cells using interferometric phase microscopy (IPM) without utilizing cell staining. (3) Results: flow cytometry results show MDS patients present different dispersion patterns compared to BBDs. BBDs present two distinct subpopulations (one of these are the H cells constituting 16% for neutrophils) whereas MDS patients present only 4% of this subpopulation. IPM results are consistent with this pattern: ~20% of BBDs neutrophils were characterized as H cells and whereas MDS patients present only ~4% of these cells. (5) Conclusion- IPM can be utilized for diagnosis of complex hematological pathologies such as MDS.
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