Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Potential of a New Bacteriophage to Control Klebsiella pneumoniae: Preliminary Studies to Control Urinary Tract Infections

João Duarte
,
Carolina Máximo
,
Pedro Costa
,
Vanessa Oliveira
ORCID logo ,
Newton CM Gomes
ORCID logo ,
Jesús López Romalde
ORCID logo ,
Carla Pereira
* ORCID logo ,
Adelaide Almeida
* ORCID logo
Version 1 : Received: 4 January 2024 / Approved: 5 January 2024 / Online: 8 January 2024 (04:20:06 CET)

A peer-reviewed article of this Preprint also exists.

Duarte, J.; Máximo, C.; Costa, P.; Oliveira, V.; Gomes, N.C.M.; Romalde, J.L.; Pereira, C.; Almeida, A. Potential of an Isolated Bacteriophage to Inactivate Klebsiella pneumoniae: Preliminary Studies to Control Urinary Tract Infections. Antibiotics 2024, 13, 195. Duarte, J.; Máximo, C.; Costa, P.; Oliveira, V.; Gomes, N.C.M.; Romalde, J.L.; Pereira, C.; Almeida, A. Potential of an Isolated Bacteriophage to Inactivate Klebsiella pneumoniae: Preliminary Studies to Control Urinary Tract Infections. Antibiotics 2024, 13, 195.

Abstract

Urinary tract infections (UTIs) caused by Klebsiella pneumoniae can lead to severe clinical complications and even death. An alternative treatment option for infected patients is using bacteriophages (phages). In the present study, we isolated phage KP-1 from sewage water using K. pneumoniae as a host. Whole genome analysis indicated that the genome was a double-stranded linear 176,096-bp long DNA molecule with 41.8% GC content and did not contain virulence or antibiotic resistance genes. The inactivation potential of phage KP-1 was assessed in broth at an MOI of 1 and 10, and a maximum inactivation of 4.9 and 5.4 log CFU/mL was observed after 9 h, respectively. The MOI of 10 efficacy was also assessed in urine to evaluate the phage’s performance in an acidic environment. A maximum inactivation of 3.8 log CFU/mL was observed after 9 h. The results indicate that phage KP-1 could be used to control UTIs caused by K. pneumoniae; however, further studies using phage cocktails to broader the phage’s spectrum of activity and phages in micro- or nanocarriers to avoid the negative effect of the low pH of the urine on phage viability, are necessary.

Keywords

Bacteriophage; Phage therapy; Klebsiella pneumoniae; Urinary tract infections

Subject

Public Health and Healthcare, Public Health and Health Services

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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