Article
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Mutations in ERG 11 Gene Among Fluconazole
Version 1
: Received: 3 January 2024 / Approved: 4 January 2024 / Online: 4 January 2024 (07:42:07 CET)
How to cite: M S, S.; Singh, R. Mutations in ERG 11 Gene Among Fluconazole. Preprints 2024, 2024010367. https://doi.org/10.20944/preprints202401.0367.v1 M S, S.; Singh, R. Mutations in ERG 11 Gene Among Fluconazole. Preprints 2024, 2024010367. https://doi.org/10.20944/preprints202401.0367.v1
Abstract
Candidiasis is one of the global public health problems. In fluconazole resistant Candida spp. (FRCS), mutations in ergosterol biosynthesis gene 11 (ERG 11) have been commonly found. This study aimed to determine the profile of FRCS isolates and to detect mutations in part of ERG 11 gene in fluconazole resistant Candida albicans (FRCA) isolates. Study was conducted on 150 isolates of FRCS obtained from patients admitted in a tertiary care hospital, South India from September 2019 to December 2021. Candida spp. was identified by MALDI-TOF MS and fluconazole resistance was detected by VITEK 2 system. Following which a part of the ERG 11 gene of the FRCA isolates were amplified, purified, sequenced and a phylogenetic tree was constructed. Out of the 150 FRCS, the most common FRCS was C. auris followed by C. glabrata, C. krusei, C. parapsilosis, C. albicans, C. tropicalis and C. guilliermondii. Sequencing of amplicons detected a single missense mutation and 44 silent mutations. We opined that fluconazole resistance in FRCA isolates may be due to mecha-nisms other than the studied one in our region. Phylogenetic analysis of amplified sequences of 12 FRCA isolates observed three clusters and all 12 sequences showed relatedness to Malaysian 2019 isolates.
Keywords
Candida albicans; ERG 11; fluconazole resistant; matrix-assisted laser desorption/ionization-time of flight mass spectrometry; MALDI TOF; VITEK 2
Subject
Medicine and Pharmacology, Epidemiology and Infectious Diseases
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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